These authors contributed equally to this work.
Loss of transforming growth factor β adaptor protein β-2 spectrin leads to delayed liver regeneration in mice †
Article first published online: 22 APR 2011
Copyright © 2011 American Association for the Study of Liver Diseases
Volume 53, Issue 5, pages 1641–1650, May 2011
How to Cite
Thenappan, A., Shukla, V., Khalek, F. J. A., Li, Y., Shetty, K., Liu, P., Li, L., Johnson, R. L., Johnson, L. and Mishra, L. (2011), Loss of transforming growth factor β adaptor protein β-2 spectrin leads to delayed liver regeneration in mice . Hepatology, 53: 1641–1650. doi: 10.1002/hep.24111
Potential conflict of interest: Dr. Shetty is on the speakers' bureau of Merck and Salix. She is also on the speakers' bureau of, and received grants from Gilead.
- Issue published online: 22 APR 2011
- Article first published online: 22 APR 2011
- Accepted manuscript online: 14 DEC 2010 03:29PM EST
- Manuscript Accepted: 18 NOV 2010
- Manuscript Received: 3 JUN 2010
- NIH Grants RO1CA042857 (to L.M.), RO1CA106614 (to L.M.), RC2-AA019392 (to L.M.), PO1CA130821 (to L.M.), Ben Orr Award (to L.M.), VA Merit grant, and Georgetown Department of Surgery Huffnagel Resident Research fund (to A.T.)
Additional Supporting Information may be found in the online version of this article.
|HEP_24111_sm_suppinfofig1-2.tif||9088K||Supporting Figure 1. Regulation of p53 expression. Western blot analysis of the p53 regulatory proteins at 24 and 48 hours post-PHx demonstrates significant depression of MDM2 in β2SP+/- mice at 48 hours and no change in PTEN expression. Supporting Figure 2. TUNEL assay for regenerating liver tissue. No evidence of TUNEL-positive apoptotic cells was noted. Representative samples from wild-type and β2SP+/- mouse livers are demonstrated from 48 hours post-PHx.|
|HEP_24111_sm_suppinfofig3-4.tif||9088K||Supporting Figure 3. Immunostaining of pRb (Ser249/Thr252), PCNA, and pH3 (Ser10) of untreated wild-type and β2SP+/- mice. Supporting Figure 4. FACS assay showed normal cell cycle profile in wild type (WT) hepatocytes, while β2SPshRNA (Mut)-transfected hepatocytes showed G2/M arrest at 0 hour and 24 hours after extraction.|
|HEP_24111_sm_suppinfotab1.doc||50K||Supporting table 1. List of Antibodies (Abs), shRNA and siRNA.|
|HEP_24111_sm_suppinfotab2.doc||48K||Supporting table 2. Expression of cell cycle proteins at different time points after PHx in wild type and β2SP+/- mouse livers.|
|HEP_24111_sm_suppinfotab3.doc||1754K||Supporting table 3. Microarray data at 48 hrs after PHx in wild type and β2SP+/- mouse livers.|
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