Additional Supporting Information may be found in the online version of this article.

HEP_24175_sm_suppinfofigure1.tif715KSupporting Figure 1. Chemokine and chemokine receptor expression profile of liver tissue Changes of chemokines and their receptors were determined in livers of 3 fra-1tg vs. wildtype animals at the age of 6 weeks using RT2 Profiler PCR Array. Results are presented as x-fold increase vs. wildtype mice.
HEP_24175_sm_suppinfofigure2.tif11210KSupporting Figure 2. Inflammatory infiltration in the liver of fra-1tg mice The composition of the portal inflammatory infiltrates of fra-1tg mice was investigated by immunohistochemistry. Neutrophils, T cells (CD3+ cells) and B cells (CD45R+ cells) were predominantly found in the portal fields. Staining for macrophages (F4/80+ cells) showed presence of Kupffer cells in the perisinusidal spaces. Representative images are shown (magnification 100times;).
HEP_24175_sm_suppinfofigure3.tif953KSupporting Figure 3: Increased profibrogenic gene expression in fra-1tg mice Relative hepatic mRNA transcript levels of the profibrotic genes PDGF-A and PDGF-C and the profibrolitic genes MMP-9, MMP-2 and TIMP-1 in fra-1tg and wildtype mice were measured by quantitative real-time PCR. Results are expressed as mean ± SEM (n-4-5/group) and in arbitrary units relative to β-actin mRNA. Data are presented as an x-fold increase vs. the corresponding wildtype controls. *P<0.05 compared to wildtype controls of the corresponding age.
HEP_24175_sm_suppinfofigure4.tif2610KSupporting Figure 4: Binding of Fra-1 to the promoters of tgfβ1, pdgf-b and pdgf-d genes Binding activity of the transcription factor Fra-1 to the promoters of tgfβ1, pdgf-b and pdgf-d genes was determined by ChIP assay. We analysed 2000 bp sequences (-2000/+1) of tgfβ1, pdgf-b and –d promoters and found 2, 3 and 1 possible binding sites for the transcription factor AP-1, respectively. Binding activity of Fra-1 in wildtype and transgenic cholangiocytes was demonstrated by PCR. As negative control an IgG antibody was used.
HEP_24175_sm_suppinfofigure5.tif1799KSupporting Figure 5: Matrix accumulation in fra-1tg x rag2-/- mice Accumulation of matrix proteins rag2-/-, fra-1tg x rag2+/- and fra-1tg x rag2-/- mice was determined by measurement of fibrosis area in 5 high power fields. Further, expression of collagen genes was determined by quantitative real time PCR. Gene expression and fibrosis area increase are presented as x-fold vs. rag2 -/-control mice.
HEP_24175_sm_suppinfo.doc48KSupporting Information

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