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Additional Supporting Information may be found in the online version of this article.

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HEP_24200_sm_suppinfofig1.tif4711KSupplemental Figure 1 Nogo-B gene deletion significantly decreases cholangiocyte in cholestatic livers. Livers were isolated from wildtype (WT) and Nogo-B KO (NGB KO) mice 4 weeks after sham or bile duct ligation (BDL) surgery. N-5-6 per group. The CK19 positive area was quantified using ImageJ. Representative images are shown.
HEP_24200_sm_suppinfofig2.tif7790KSupplemental Figure 2 Nogo-B gene deletion does not affect Kupffer cell infiltration in the liver after bile duct ligation (BDL). No differences were seen in F4/80 immunostaining, a Kupffer cell marker, four weeks after BDL. Representative images of F4/80 staining are shown. Arrows; F4/80 positive Kupffer cells. Scale bar; 200 μm.
HEP_24200_sm_suppinfofig3.tif6366KSupplemental Figure 3 Nogo-B is localized to non-parenchymal cells in regions of fibrotic areas of cholestatic liver. Nogo-B in red and β-smooth muscle actin (?-SMA) in green. Nogo-B staining is prominent in fibrotic areas (arrow), endothelial cells (asterisks; vessel lumen) and bile ducts (arrow head). Corresponding magnified images of the upper panel are shown below. Scale bar; 200 μm.

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