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Liver Biology/Pathobiology

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Hepatic differentiation of amniotic epithelial cells†‡§

  1. Fabio Marongiu1,4,
  2. Roberto Gramignoli1,
  3. Kenneth Dorko1,
  4. Toshio Miki1,2,
  5. Aarati R. Ranade1,
  6. Maria Paola Serra4,
  7. Silvia Doratiotto4,
  8. Marcella Sini4,
  9. Shringi Sharma3,
  10. Keitaro Mitamura1,
  11. Tiffany L. Sellaro2,
  12. Veysel Tahan1,
  13. Kristen J. Skvorak1,
  14. Ewa C. S. Ellis1,5,
  15. Stephen F. Badylak2,
  16. Julio C. Davila6,
  17. Ronald Hines7,
  18. Ezio Laconi4,
  19. Stephen C. Strom1,*

Article first published online: 22 APR 2011

DOI: 10.1002/hep.24255

Issue

Hepatology

Hepatology

Volume 53, Issue 5, pages 1719–1729, May 2011

Additional Information

How to Cite

Marongiu, F., Gramignoli, R., Dorko, K., Miki, T., Ranade, A. R., Paola Serra, M., Doratiotto, S., Sini, M., Sharma, S., Mitamura, K., Sellaro, T. L., Tahan, V., Skvorak, K. J., Ellis, E. C. S., Badylak, S. F., Davila, J. C., Hines, R., Laconi, E. and Strom, S. C. (2011), Hepatic differentiation of amniotic epithelial cells. Hepatology, 53: 1719–1729. doi: 10.1002/hep.24255

Author Information

  1. 1

    Department of Pathology, the, University of Pittsburgh, Pittsburgh, PA

  2. 2

    McGowan Institute for Regenerative Medicine, and the, University of Pittsburgh, Pittsburgh, PA

  3. 3

    Department of Pharmaceutical Sciences, University of Pittsburgh, Pittsburgh, PA; the

  4. 4

    Department of Biomedical Sciences and Technologies, University of Cagliari, Italy; the

  5. 5

    Department of Clinical Science, Intervention and Technology, Karolinska Institute, Stockholm, Sweden

  6. 6

    Pfizer Inc., Pfizer Global Research and Development, St. Louis, MO

  7. 7

    Children's Research Institute, Medical College of Wisconsin, Milwaukee, WI

*Department of Pathology, University of Pittsburgh, 200 Lothrop St., 450 BST South, Pittsburgh, PA 15261

  1. †

    Potential conflict of interest: Stephen C. Strom owns stock in Stemnion, LLC.

  2. ‡

    17-OHPC, 17-hydroxyprogesterone caproate; A1AT, α-1 anti-trypsin; BNF, β-naphtoflavone; CYP, cytochrome P450; Dex, dexamethasone; DMEM, Dulbecco's modified Eagle's medium; DPPIV, dipeptidyl peptidase type IV; ECM, extracellular matrix; EGF, epidermal growth factor; ESC, embryonic stem cell; FBS, fetal bovine serum; hAEC, human amniotic epithelial cell; FGF2, fibroblast growth factor 2; IMDM, Iscove's modified Dulbecco's medium; L-ECM, liver-derived extracellular matrix; mHep, mouse hepatocyte; mRNA, messenger RNA; PB, phenobarbital; qRT-PCR, quantitative reverse-transcription polymerase chain reaction; rAEC, rat amniotic epithelial cell; RS, retrorsine; Rif, rifampicin; SCID, severe combined immunodeficient; Std, standard supplement; UGT1A, 5'-diphospho-glucuronosyltransferase 1 family, type A.

  3. §

    Supported in part by a grant from Pfizer, Inc. Studies with adult human liver and hepatocytes were supported in part by National Institutes of Health (NIH) Grants N01-DK-7-0004/HHSN26700700004C and RC1DK086135 (to S. C. S.). Fetal human liver tissue was provided as a service from the Laboratory of Developmental Biology at the University of Washington, which was supported by NIH Award Number 5R24HD0008836 from the Eunice Kennedy Shriver National Institute of Child Health & Human Development. Studies with fetal human liver were supported in part by NIH Grant R01-GM081344.

Publication History

  1. Issue published online: 22 APR 2011
  2. Article first published online: 22 APR 2011
  3. Accepted manuscript online: 3 MAR 2011 08:58AM EST
  4. Manuscript Accepted: 11 FEB 2011
  5. Manuscript Received: 15 NOV 2010

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