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Additional Supporting Information may be found in the online version of this article.

FilenameFormatSizeDescription
HEP_24269_sm_suppinfofig1.tif2424KSupporting Figure 1. Colocalization of apoB with LC3 in McA-RH7777 cells. (A and B) Confocal microscopy photographs showing co- localization of apoB (b, e, h, red) with LC3 (a, d, g, green), and apoB-LC3 puncta (c, f, i, yellow), in McA-RH7777 cells following treatment with tunicamycin (TM) or glucosamine (GLS). Scale bar: 17 μM. (C) Data analysis is shown percentage of apoB-LC3 positive cells, and the number of apoB-LC3 puncta in positive cells; n= 3, *p<0.05 vs corresponding control.
HEP_24269_sm_suppinfofig2.tif1743KSupporting Figure 2. Colocalization of apoB with LC3 in primary rat hepatocytes. (A and B) Confocal microscopy photographs showing co- localization of apoB (b, e, h, red) with LC3 (a, d, g, green), and apoB-LC3 puncta (c, f, i, yellow), in primary rat hepatocytes following treatment with tunicamycin (TM) or glucosamine (GLS). Scale bar: 11 μM. (C) Data analysis is shown percentage of apoB-LC3 positive cells, and the number of apoB-LC3 puncta in positive cells; n= 3, *p<0.05 vs corresponding control.
HEP_24269_sm_suppinfofig3.tif16854KSupporting Figure 3. ER stress-induced colocalization of apoB with GFP-LC3 is cell type dependent. (A) Confocal microscopy photographs showing co- localization of apoB (b, e, h, red) with GFP-LC3 (a, d, g, green), and apoB-GFP-LC3 puncta (c, f, i, yellow), in primary hamster hepatocytes following treatment with tunicamycin (TM) or glucosamine (GLS). Scale bar: 14 μM. (B) Data analysis from (A), showing percentage of apoB-GFP-LC3 positive cells, and the number of apoB-GFP-LC3 puncta in positive cells; 3 independent experiments, *p<0.05 vs corresponding control. (C and D) The colocalization of apoB (red) with GFP-LC3 (green) in HepG2 cells in the presence of ALLN (20 μg/ml) plus oleic acid (0.4 mM). Scale bar: 7μM. (E) Data analysis from (D); 3 independent experiments, *p<0.05 vs oleate alone.
HEP_24269_sm_suppinfofig4.tif1955KSupporting Figure 4. PBA does not prevent DHA or ALLN induced apoB autophagy in primary rat hepatocytes. Confocal microscopy photographs showing colocalization of apoB (b, e, h, red) with GFP-LC3 (a, d, g, green), and apoB-GFP-LC3 puncta (c, f, i, yellow), in primary rat hepatocytes following treatment with DHA or ALLN. (A) no PBA treatment and (B) treated with PBA. Scale bar: 11 μM. Data analysis is shown in (C) showing percentage of apoB-GFP-LC3 positive cells, and (D) shows the number of apoB-GFP-LC3 puncta in the positive cells; 3 independent experiments, *p<0.05.
HEP_24269_sm_suppinfotab1.doc341KSupporting Table 1. cDNA sequence of primers used in RT-PCR for detection of apoB, apoE, apoA1,GRP78, Xbp1, PERK and ATF6 mRNA levels.

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