SEARCH

SEARCH BY CITATION

Additional Supporting Information may be found in the online version of this article.

FilenameFormatSizeDescription
HEP_24329_sm_suppinfo.doc33KSupporting Information
HEP_24329_sm_suppinfofig1.tif1997KSupporting Fig. 1. Expression of constitutively active (ca) Stat3 versions and U-Stat3 in Ras-transformed hepatocytes. (A) Schematic representation of wtStat3 and Stat3 mutants. Stat3 is composed of the N-terminal domain, the coiled-coil, DNA-binding, linker, Src homology 2 (SH2) and transcriptional activation domain (TAD). (B) Overexpression of Stat3 as shown by immunoblotting using anti-Stat3 and anti-Actin antibodies. Actin was used as loading control.
HEP_24329_sm_suppinfofig2.tif1767KSupporting Fig. 2. Characteristics of Stat3Δhc/p19ARF-/- hepatocytes. (A) The loss of Stat3 and p19ARF in single and double knocked-out hepatocytes was analyzed by linear semi-quantitative RT-PCR (wt, wild type; ko, knock-out). (B) pY-Stat3 levels were analyzed by immunoblotting after stimulation with IL-6 (20 ng/ml). Actin was used as loading control. (C) Hepatocellular marker expression analyzed by linear semi-quantitative RT-PCR in primary hepatocytes (prim. hep.), parental MIM-1-4 hepatocytes (MIM-1-4) and the two single cell clones MIM-Stat3Δhc-1 and MIM-Stat3Δhc-2. The constitutive expression of RhoA is shown as loading control. One representative out of three experiments is shown. HNF, hepatocyte nuclear factor.
HEP_24329_sm_suppinfofig3.tif9550KSupporting Fig. 3. Knock-down of p14ARF in human Hep3B cells. (A) Real-time PCR analysis of p14ARF in p14ARF-deficient MCM-1, p14ARF-positive SW480 and Hep3B cells. (B) Levels of p14ARF and pY-Stat3 in Hep3B cells after expression of either scrambled control shRNA (sh-control) or shRNAs against p14ARF (sh-p14-1, sh-p14-2, sh-p14-3) as determined by immunoblot analysis. Actin was used as loading control. (C) Phase contrast and confocal immunofluorescence images after staining of Hep3B cells and those expressing sh-control or sh-p14 with anti-p14ARF antibody. Red, phalloidin; green, p14ARF; blue, DNA. Arrows indicate p14ARF localization in cell nucleoli. Error bars depict SD from at least three individual experiments.

Please note: Wiley Blackwell is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.