These authors contributed equally to this work.
Version of Record online: 24 JUN 2011
Copyright © 2011 American Association for the Study of Liver Diseases
Volume 54, Issue 1, pages 164–172, July 2011
How to Cite
Schneller, D., Machat, G., Sousek, A., Proell, V., van Zijl, F., Zulehner, G., Huber, H., Mair, M., Muellner, M. K., Nijman, S. M.B., Eferl, R., Moriggl, R. and Mikulits, W. (2011), p19ARF/p14ARF controls oncogenic functions of signal transducer and activator of transcription 3 in hepatocellular carcinoma. Hepatology, 54: 164–172. doi: 10.1002/hep.24329
Potential conflict of interest: Nothing to report.
This work was supported by the Austrian Science Fund, FWF, grant numbers SFB F28 (to R.E., R.M., and W.M.), P19598 (to W.M.), and P20905 (to W.M.), and the European Union, FP7 Health Research, project number HEALTH-F4-2008-202047 (to W.M.).
See Editorial on Page 9
- Issue online: 24 JUN 2011
- Version of Record online: 24 JUN 2011
- Accepted manuscript online: 30 MAR 2011 11:31AM EST
- Manuscript Accepted: 22 MAR 2011
- Manuscript Received: 2 FEB 2011
Additional Supporting Information may be found in the online version of this article.
|HEP_24329_sm_suppinfofig1.tif||1997K||Supporting Fig. 1. Expression of constitutively active (ca) Stat3 versions and U-Stat3 in Ras-transformed hepatocytes. (A) Schematic representation of wtStat3 and Stat3 mutants. Stat3 is composed of the N-terminal domain, the coiled-coil, DNA-binding, linker, Src homology 2 (SH2) and transcriptional activation domain (TAD). (B) Overexpression of Stat3 as shown by immunoblotting using anti-Stat3 and anti-Actin antibodies. Actin was used as loading control.|
|HEP_24329_sm_suppinfofig2.tif||1767K||Supporting Fig. 2. Characteristics of Stat3Δhc/p19ARF-/- hepatocytes. (A) The loss of Stat3 and p19ARF in single and double knocked-out hepatocytes was analyzed by linear semi-quantitative RT-PCR (wt, wild type; ko, knock-out). (B) pY-Stat3 levels were analyzed by immunoblotting after stimulation with IL-6 (20 ng/ml). Actin was used as loading control. (C) Hepatocellular marker expression analyzed by linear semi-quantitative RT-PCR in primary hepatocytes (prim. hep.), parental MIM-1-4 hepatocytes (MIM-1-4) and the two single cell clones MIM-Stat3Δhc-1 and MIM-Stat3Δhc-2. The constitutive expression of RhoA is shown as loading control. One representative out of three experiments is shown. HNF, hepatocyte nuclear factor.|
|HEP_24329_sm_suppinfofig3.tif||9550K||Supporting Fig. 3. Knock-down of p14ARF in human Hep3B cells. (A) Real-time PCR analysis of p14ARF in p14ARF-deficient MCM-1, p14ARF-positive SW480 and Hep3B cells. (B) Levels of p14ARF and pY-Stat3 in Hep3B cells after expression of either scrambled control shRNA (sh-control) or shRNAs against p14ARF (sh-p14-1, sh-p14-2, sh-p14-3) as determined by immunoblot analysis. Actin was used as loading control. (C) Phase contrast and confocal immunofluorescence images after staining of Hep3B cells and those expressing sh-control or sh-p14 with anti-p14ARF antibody. Red, phalloidin; green, p14ARF; blue, DNA. Arrows indicate p14ARF localization in cell nucleoli. Error bars depict SD from at least three individual experiments.|
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