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Additional Supporting Information may be found in the online version of this article.

FilenameFormatSizeDescription
HEP_24352_sm_suppinfofig1.tif1256KSupporting Figure 1: HSC veto function does not impair APC function of DCs. CFSE dilution profiles of naïve OT-1 T cells at d3 after incubation with H-2Kb DCs pulsed with SIINFEKL (S8L, 1 μM) ± H-2bm1 HSCs (a) or Des-TCR T cells incubated with H-2Kb DCs expressing endogenous peptides ± H-2d HSCs. (b) Histograms reflect one out of two experiments and numbers denote division indices (DI).
HEP_24352_sm_suppinfofig2.tif1293KSupporting Figure 2: HSC veto function is independent from genetic background and species. CFSE dilution profiles of (a) αCD3/28 bead-stimulated naïve CD8+ T cells from Balb/c mice (H-2d) with H-2d HSCs and (b) αCD3/28 bead-stimulated naïve CD8+ T cells ± LX-2 at d3 after incubation. One representative of two independent experiments is shown; numbers denote division indices (DI).
HEP_24352_sm_suppinfofig3.tif533KSupporting Figure 3: Kidney fibroblasts show a similar veto effect to HSCs. CFSE dilution profiles of αCD3/28 bead-stimulated naïve CD8+ T cells in presence of primary kidney fibroblasts (FB). One representative of three independent experiments is shown; numbers denote division indices (DI).
HEP_24352_sm_suppinfofig4.tif983KSupporting Figure 4: No contribution of soluble regulatory molecules. CFSE dilution profiles of T cells after incubation ± αCD3/28 beads with wild-type HSCs in presence of neutralizing antibodies to IL-6 (30 μg/ml), IL-10 (30 μg/ml), TGF-β (30 μg/ml). One representative of two independent experiments is shown; numbers denote division indices (DI).
HEP_24352_sm_suppinfofig5.tif643KSupporting Figure 5: Contribution of CD106 to HSC veto effect. CFSE dilution profiles of T cells after incubation ± αCD3/28 beads with wild-type HSCs in presence of neutralizing antibodies to CD106 (30 μg/ml). One representative of two independent experiments is shown; numbers denote division indices (DI).
HEP_24352_sm_suppinfofig6.tif445KSupporting Figure 6: Transfection efficiency in αML. CD54 surface expression levels by flow cytometry on αML after lipofection (dashed lines). Untreated cells (solid lines) and isotype antibody (shaded) were used as controls. Histograms reflect one out of three experiments.

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