Steatohepatitis/Metabolic Liver Disease
Article first published online: 23 JUN 2011
Copyright © 2011 American Association for the Study of Liver Diseases
Volume 54, Issue 2, pages 495–508, August 2011
How to Cite
Cinaroglu, A., Gao, C., Imrie, D. and Sadler, K. C. (2011), Activating transcription factor 6 plays protective and pathological roles in steatosis due to endoplasmic reticulum stress in zebrafish. Hepatology, 54: 495–508. doi: 10.1002/hep.24396
Potential conflict of interest: Nothing to report.
Kirsten C. Sadler was supported by the American Gastrological Association (through the Research Scholar Award to Kirsten C. Sadler), the March of Dimes (through the Basil O'Connor Award), and the National Institute on Alcohol Abuse and Alcoholism (through grants p20AA017067-01 to S. L. Friedman and 1RO1AA18886-01), and Dru Imrie was partially supported by the Medical Scientist Training Program (through grant T32GM007280) and the Training Program in Cellular and Molecular Biology of the National Institute of General Medical Sciences (through grant T32GM08633).
- Issue published online: 25 JUL 2011
- Article first published online: 23 JUN 2011
- Accepted manuscript online: 29 APR 2011 11:10AM EST
- Manuscript Accepted: 19 APR 2011
- Manuscript Received: 3 JAN 2011
Additional Supporting Information may be found in the online version of this article.
|HEP_24396_sm_suppinfofig1.tif||2827K||Supporting Figure 1. There are no measureable differences between control morpholino injection and uninjected larva. A. Embryo and larval morphology and survival (not shown) are the same in uninjected (top) and control morpholino injected (bottom). Live images are shown. Scale bar = 1 mm. B. Liver size, shape and steatosis is the same in 5 dpf uninjected Tg(fabp10:dsRed) larvae and those injected with the control morpholino. Steatosis was scored based on oil red O staining in 3 clutches. C. UPR and Srebp target gene expression is not affected by control morpholino injection. qPCR analysis of 5 dpf control morphants compared to uninjected controls is plotted as the average fold change from 3 experiments with bars showing the standard deviation.|
|HEP_24396_sm_suppinfofig2.tif||5568K||Supporting Figure 2. Srebp2 target genes are upregulated in atf6 morphants. atf6 morphants were collected as whole larvae or the liver was dissected on 5 dpf and analyzed using qPCR. The ΔCt (normalized to rpp0) calculated for each clutch was plotted as an individual dot, with the mean indicated by the horizontal bar. * indicates p<0.05; ** indicates p< 0.005 by students t-test.|
|HEP_24396_sm_suppinfotab1.doc||96K||Supporting Information Table 1.|
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