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Additional Supporting Information may be found in the online version of this article.

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HEP_24465_sm_suppinfo.pdf88KSupporting Information
HEP_24465_sm_suppinfoFigs.pdf107KSupporting Information Fig. 1 Levels of NOX2 and NOX4 mRNAs in the liver at day 10 after BDL. N = 5 - 6 per group. **p < 0.01 vs. corresponding control groups.; Supporting Fig. 2 Levels of PDGF, TGF-β1, MCP1 and TNFα mRNAs in the liver at day 10 after BDL. Transcript levels were determined by real-time PCR. N = 5 - 7 per group. *p < 0.05, **p < 0.01 vs. corresponding control groups.; Supporting Fig. 3 Levels of NOX1 (A) and col-1α mRNAs (B) in the liver after CCL4 treatment for 8 weeks. N = 4 - 6 per group. **p < 0.01 vs. corresponding control groups. (C) Representative immunoblots and quantitative densitometric analysis of α-SMA in the liver of CCL4 model. N = 4 - 6 per group. **p < 0.01 vs. corresponding control groups.; Supporting Fig. 4 Effects of bile acid on the level of NOX1 mRNA and cleaved caspase-3 in isolated hepatocytes. Forty-eight hours after preparation, primary cultured hepatocytes were stimulated with 200μM chenodeoxycholic acid for 4 hours. (A) Level of NOX mRNA was measured by real-time PCR. N = 3 per group. (B) Representative immunoblots and quantitative densitometric analysis of cleaved caspase-3. N = 4 - 6. **p < 0.01 vs. corresponding control groups, ##p < 0.01 vs. corresponding WT group.; Supporting Fig. 5 Levels of col-1α, α-SMA, RANTES and MCP1 mRNAs in primary cultured HSCs at day 14. N = 5.; Supporting Fig. 6 (A) Increased expression of NOX1 mRNA under serum starvation. HSCs at day 14 in culture were incubated in serum-free medium for 8 hours (SS 8hr). NOX1 mRNA level was determined by real-time PCR. N = 3. *p < 0.05 vs. control. (B) Immunoblot analysis of EGFR in HSCs isolated from WT and Nox1KO at day 14 in culture. N = 4.

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