fax: (203) 785-7273
Version of Record online: 25 AUG 2011
Copyright © 2011 American Association for the Study of Liver Diseases
Volume 54, Issue 3, pages 890–899, 2 September 2011
How to Cite
Fabris, L., Cadamuro, M., Moserle, L., Dziura, J., Cong, X., Sambado, L., Nardo, G., Sonzogni, A., Colledan, M., Furlanetto, A., Bassi, N., Massani, M., Cillo, U., Mescoli, C., Indraccolo, S., Rugge, M., Okolicsanyi, L. and Strazzabosco, M. (2011), Nuclear expression of S100A4 calcium-binding protein increases cholangiocarcinoma invasiveness and metastasization. Hepatology, 54: 890–899. doi: 10.1002/hep.24466
Potential conflict of interest: Nothing to report.
Supported by Telethon (grant GGP09189) and Federazione Amici Di Epatologia (FADE) to L.F. Supported by Progetto di Ricerca Ateneo 2008 (grant CPDA083217 to L.F., M.C., and L.O.). Associazione Scientifica Gastroenterologica di Treviso (ASGET, “associazione di promozione sociale senza scopo di lucro” to L.F. and L.O.). Yale University Liver Center (NIH DK34989 to M.S.). Fondazione S. Martino, Bergamo.
- Issue online: 25 AUG 2011
- Version of Record online: 25 AUG 2011
- Accepted manuscript online: 26 MAY 2011 09:41AM EST
- Manuscript Accepted: 16 MAY 2011
- Manuscript Received: 25 NOV 2010
- Telethon. Grant Number: GGP09189
- Federazione Amici Di Epatologia (FADE)
- Progetto di Ricerca Ateneo 2008. Grant Number: CPDA083217
- Associazione Scientifica Gastroenterologica di Treviso (ASGET, “associazione di promozione sociale senza scopo di lucro”
- Yale University Liver Center
- NIH. Grant Number: DK34989
- Fondazione S. Martino, Bergamo
Additional Supporting Information may be found in the online version of this article.
|HEP_24466_sm_SuppInfoFigS1.tif||1271K||Figure S1. Hazard functions for death and metastasis with Weibull model. The estimated hazard function for death (A) and metastasis (B) are shown. For death, the hazard function was increased with a rate decreasing over time (A), whereas for metastasis, the hazard is very high at the beginning but it rapidly decreases over time (B).|
|HEP_24466_sm_SuppInfoFigS2.tif||4355K||Figure S2. Immunocytochemical expression of K19 in human CCA cell lines is not altered by lentiviral silencing of S100A4. K19 expression (green) was similar in EGI-1 (A), sh8-EGI-1 (B) and sh9-EGI-1 (C) cells as well as in TFK-1 (D) cell line. Nuclei were counterstained with DAPI (blue). Original magnification: 200×.|
|HEP_24466_sm_SuppInfoFigS3.tif||625K||Figure S3. Functional effects of S100A4 silencing in EGI-1 cells as compared to TFK-1 cells on cell proliferation. Using MTS assay after a 24h incubation with growth medium, no difference in the proliferative activity was found among silenced, scramble, EGI-1 and TFK-1 cells.|
|HEP_24466_sm_SuppInfoFigS4.tif||2193K||Figure S4. MMP-2 and MMP-9 expression in EGI-1 cells and functional effects of S100A4 silencing in EGI-1 cells on MMP-9 secretion. Tissue sections of liver metastases derived from xenotransplantation of EGI-1 cells in SCID mice immunostained for MMP-2 (A) and MMP-9 (B) showed that MMP-9, but MMP-2, was strongly expressed by metastasizing CCA cells. Immunoperoxidase technique, original magnification: 200×. In the supernatant of cultured cells (n×4), MMP-9 secretion by EGI-1 cells assessed by ELISA was significantly reduced after lenitiviral silencing of S100A4, as observed in sh8 and sh9 clones (p<0.001); in TFK-1 cells, MMP-9 secretion is negligible (C).|
|HEP_24466_sm_SuppInfoTableS1.doc||39K||Table S1. Summary of survival for each group in Kaplan-Meier survival curves.|
|HEP_24466_sm_SuppInfoTableS2.doc||72K||Table S2. Clinical features of the CCA series (n=67) according to S100A4 grouping considered for NPMLE analysis and Weibull model a.|
|HEP_24466_sm_SuppInfoTableS3.doc||51K||Table S3. Results of Weibull Model with outcome as death, in 67 resected human CCA.|
Please note: Wiley Blackwell is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.