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Additional Supporting Information may be found in the online version of this article.

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HEP_24488_sm_suppinfoFigS1.tif192KSupporting Information Figure S1. AOAH-deficient mice recover from hepatomegaly induced by an agonistic antibody to MD-2—TLR4. A. UT12-induced hepatomegaly is dose-dependent. Aoah-/- (open squares) and Aoah+/+ (closed squares) mice were injected i.v. with different amounts of UT12 antibody. Livers were excised and weighed 7 days later. B. UT12 induces transient hepatomegaly in both Aoah-/- and Aoah+/+ mice. Aoah-/- and Aoah+/+ mice were injected i.v. with UT12 (0.1 μg/g body weight) and groups of 3 mice were euthanized on days 7, 14 and 21. The baseline values (day 0) were derived from mice that received PBS instead of UT12 or LPS. C. Dose response for LPS-induced hepatomegaly in Aoah+/+ and Aoah-/- mice. D. Time course of LPS-induced, hepatomegaly in Aoah+/+ and Aoah-/- mice. Mice (three per group) were injected with 10 ug LPS/mouse i.v., and the liver to body weight ratio determined at 7, 14 and 21 days. Note that this dose is not sufficient to cause significant hepatomegaly in Aoah+/+ mice.
HEP_24488_sm_suppinfoFigS2.tif607KSupporting Information Figure S2. Hepatocyte proliferation does not contribute to prolonged hepatomegaly. Aoah-/- and Aoah+/+ mice were injected i.v. with 0.5 μg of LPS per g body weight, PBS or 0.4 mg/kg of TCPOBOP. Two hours later, Brdu (1 mg / mouse) was given i.p. and once daily to the end of experiment at day 7. Liver sections were stained with anti-Brdu antibody and propidium iodide (PI). Images were taken using a Zeiss Axioplan 2 fluorescence microscope. Brdu (green) and PI (red) positive cells were quantitated from 6 images of each group. The labeling index is expressed as number of Brdu-positive cells/100 nuclei. The labeling index indicates the level of liver cell proliferative response.
HEP_24488_sm_suppinfoFigTables.doc83KSupporting Information Tables

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