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Article first published online: 29 JUL 2011
Copyright © 2011 American Association for the Study of Liver Diseases
Volume 54, Issue 4, pages 1237–1248, October 2011
How to Cite
Kogure, T., Lin, W.-L., Yan, I. K., Braconi, C. and Patel, T. (2011), Intercellular nanovesicle-mediated microRNA transfer: A mechanism of environmental modulation of hepatocellular cancer cell growth. Hepatology, 54: 1237–1248. doi: 10.1002/hep.24504
Potential conflict of interest: Nothing to report.
This work was supported, in part, by Grant DK069370 from the National Institutes of Health (Bethesda, MD).
- Issue published online: 27 SEP 2011
- Article first published online: 29 JUL 2011
- Accepted manuscript online: 30 JUN 2011 07:11PM EST
- Manuscript Accepted: 7 JUN 2011
- Manuscript Received: 16 FEB 2011
Additional Supporting Information may be found in the online version of this article.
|HEP_24504_sm_suppinfofig1.tif||1333K||Supplementary Figure 1. Protein expression in isolated vesicles. PLC/PRF/5 derived particles were conjugated with 4 μm-aldehyde/sulfate latex beads (Invitrogen) washed with PBS containing 1% bovine serum albumin and stained with primary antibodies against CD63, COX-IV, Calnexin, PMP70 (red) or iso-type controls (black) followed by FITC- or PE-labeled secondary antibodies and analysis using an Accuri C6 flow cytometer (Accuri Cytometers).|
|HEP_24504_sm_suppinfofig2.tif||817K||Supplementary Figure 2. RNA within CNP is resistant to RNase degradation. CNPs were isolated from Hep3B cells, resupended in PBS, and separated into three aliquots. For the first two aliquots, RNA was isolated after incubation of CNP with either 100 μg/ml of RNase A or diluent control. For the third aliquot, total RNA was first isolated, then incubated with 100 μg/ml of RNase A. The RNA was then analyzed using a NanoDrop ND-1000 spectrophotometer. A. The bars indicate the mean ± SEM for the yield of RNA / μg of CNPs from three independent experiments*, p < 0.05. (B) Representative absorbance curves of RNA obtained from each of the three aliquots.|
|HEP_24504_sm_suppinfotable1.xls||42K||Supporting Information Table 1.|
|HEP_24504_sm_suppinfotable2.xls||41K||Supporting Information Table 2.|
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