These authors contributed equally to this work.
Article first published online: 30 NOV 2011
Copyright © 2011 American Association for the Study of Liver Diseases
Volume 54, Issue 6, pages 2025–2035, December 2011
How to Cite
Yuan, J.-h., Yang, F., Chen, B.-f., Lu, Z., Huo, X.-s., Zhou, W.-p., Wang, F. and Sun, S.-h. (2011), The histone deacetylase 4/SP1/microrna-200a regulatory network contributes to aberrant histone acetylation in hepatocellular carcinoma. Hepatology, 54: 2025–2035. doi: 10.1002/hep.24606
Potential conflict of interest: Nothing to report.
This study was supported by grant 81071680 from the National Natural Science Foundation of China and by grant 2011ZXJ09103-07C from the Key New Drug Creation Program.
- Issue published online: 30 NOV 2011
- Article first published online: 30 NOV 2011
- Accepted manuscript online: 11 AUG 2011 09:28AM EST
- Manuscript Accepted: 30 JUL 2011
- Manuscript Received: 9 MAY 2011
Additional Supporting Information may be found in the online version of this article.
|HEP_24606_sm_SuppFig1.tif||846K||Supporting Fig. 1. CpG islands of miR-200a locus in HCC and matched controls are both hypermethylated. Methylation pattern of miR-200a locus was analyzed by bisulfite sequencing. Shown is an example of the five HCC samples undergone epigenetic research (p = 0.28, Wilcoxon rank-sum test). The other samples showed a similar manner.|
|HEP_24606_sm_SuppFig2.tif||4635K||Supporting Fig. 2. Effects of HDAC4 on the expression of the miR-200 family. (A) The expression of the miR-200 family in SMMC-7721 and HepG2 cells. (B) Expression level of the miR-200 family after the transfection of pcDNA3.1-HDAC4 or pcDNA3.1 into SMMC-7721 and HepG2 cells. (C) Expression level of the miR-200 family after the transfection of HDAC4 siRNA or the control siRNA into SMMC-7721 and HepG2 cells. Data are presented as the mean ± standard error, based on at least three independent experiments. *P < 0.05.|
|HEP_24606_sm_SuppFig3.tif||1205K||Supporting Fig. 3. Effects of the miR-200 family on the expression of HDAC4. The amounts of protein were quantified and indicated relative to the signals from miRNAs negative control-transfected cells (set to 1). Data are presented as the mean of three independent experiments.|
|HEP_24606_sm_SuppTable1.doc||83K||Supporting Table 1. Oligonucleotide Sequences used in this study|
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