SEARCH

SEARCH BY CITATION

Additional Supporting Information may be found in the online version of this article.

FilenameFormatSizeDescription
HEP_24763_sm_SuppFig1.tif1967KSupporting Information Figure 1. HCV-induced NF-κB complexes in Huh7.5, 7.5-TLR3 and 7.5-N541A cells. Nuclear extracts were isolated from cells mock-infected or infected with JFH1 virus for 48 h and subjected to EMSA analysis of NF-κB complex formation. The positions of the p65/p50 heterodimer and p50/p50 homodimer are marked by arrow head and empty circle, respectively.
HEP_24763_sm_SuppFig2.tif8851KSupporting Information Figure 2. Co-localization of TLR3 and HCV dsRNAs in HCV infected cells. Huh7-TLR3 cells infected with JFH1 virus were fixed and immunostained for TLR3 (green fluorescence, using anti-Flag pAb) and dsRNA (red fluorescence, using anti-dsRNA J2 mAb), respectively. Nuclei (blue fluorescence) were counterstained with DAPI. Representative confocal images are shown.
HEP_24763_sm_SuppFig3.tif2392KSupporting Information Figure 3. TLR3 signaling in cultured hepatocytes is abrogated by inhibition of autophagy. 7.5-TLR3 (left) and PH5CH8 (right) cells were mock-stimulated or stimulated by 50 μg/ml poly-I:C in the presence or absence of 5 mM 3-MA. 6 h later, cells were harvested for qPCR analysis of RANTES mRNA induction (upper panels). The lower panel shows immunoblotting of ISG56 expression in mock-stimulated or poly-I:C-stimulated 7.5-TLR3 cells in the presence or absence of 3-MA. Asterisk denotes a nonspecific band that served as a loading control.
HEP_24763_sm_SuppFig4.tif2701KSupporting Information Figure 4. TLR3 signaling in cultured hepatocytes is blocked by inhibition of endolysosome acidification. (A) Huh7.5 cells expressing TLR3 were mock-treated or stimulated by 50 μg/ml poly-I:C in the presence or absence of 100 nM Bafilomycin A1 (Baf-A1). The expression of ISG15, ISG56 and actin was determined by immunoblotting. (B) poly-I:C activation of the NF-?B-dependent PRDII promoter in PH5CH8 cells in the presence of Baf-A1 or solvent control (DMSO). (C) PH5CH8 cells were mock-treated or stimulated by poly-I:C in the presence or absence of 100 nM Baf-A1. The expression of ISG15 and actin was determined by immunoblotting.
HEP_24763_sm_SuppInfo.doc76KSupporting Information and Tables.

Please note: Wiley Blackwell is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.