SEARCH

SEARCH BY CITATION

Additional Supporting Information may be found in the online version of this article.

FilenameFormatSizeDescription
HEP_25594_sm_SuppFig1.tif7015KSupplemental Fig. 1. Effect of silencing C/EBPβ on MAT2A expression . A. RSG-treated BSC cells were transfected with an siRNA against C/EBPβ or a negative control siRNA as described in “Experimental procedures”. Total RNA was assessed by real-time PCR for the expression of C/EBPβ or MAT2A. Results represent mean ± S.E. from three experiments in duplicates. *P<0.005, **p<0.05 vs. negative control siRNA. B. Total protein was subjected to western blotting with antibodies against C/EBPβ and MAT2A. Representative images and densitometric analysis (mean ± S.E.) from three experiments in duplicates is shown. **p<0.05 vs. negative control siRNA. C. C/EBPβ knockdown was performed in RSG-treated BSC cells followed by transfection with the MAT2A promoter or pGL3-Basic vector. The luciferase activity in C/EBPβ knockdown cells was normalized to that of pGL3-basic and expressed as a fold of negative control siRNA. Results represent mean ± S.E. from four experiments in duplicates, **p<0.05 vs. negative control siRNA.
HEP_25594_sm_SuppTab1.doc31KSUPPLEMENTAL TABLE I. Primers used to generate PPRE deletion mutants of the rat MAT2A promoter.
HEP_25594_sm_SuppTab2.doc30KSUPPLEMENTAL TABLE II. Sequence of PPRE probes for EMSA analysis.

Please note: Wiley Blackwell is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.