These authors contributed equally to this manuscript.
Early transcriptional programming links progression to hepatitis C virus–induced severe liver disease in transplant patients†
Article first published online: 5 JUN 2012
Copyright © 2012 American Association for the Study of Liver Diseases
Volume 56, Issue 1, pages 17–27, July 2012
How to Cite
Rasmussen, A. L., Tchitchek, N., Susnow, N. J., Krasnoselsky, A. L., Diamond, D. L., Yeh, M. M., Proll, S. C., Korth, M. J., Walters, K.-A., Lederer, S., Larson, A. M., Carithers, R. L., Benecke, A. and Katze, M. G. (2012), Early transcriptional programming links progression to hepatitis C virus–induced severe liver disease in transplant patients. Hepatology, 56: 17–27. doi: 10.1002/hep.25612
Potential conflict of interest: Nothing to report.
- Issue published online: 3 JUL 2012
- Article first published online: 5 JUN 2012
- Accepted manuscript online: 25 JAN 2012 05:21AM EST
- Manuscript Accepted: 15 JAN 2012
- Manuscript Received: 27 SEP 2011
- National Institute on Drug Abuse. Grant Number: 1P30DA01562501
- Top of page
- Materials and Methods
- Supporting Information
Liver failure resulting from chronic hepatitis C virus (HCV) infection is a major cause for liver transplantation worldwide. Recurrent infection of the graft is universal in HCV patients after transplant and results in a rapid progression to severe fibrosis and end-stage liver disease in one third of all patients. No single clinical variable, or combination thereof, has, so far, proven accurate in identifying patients at risk of hepatic decompensation in the transplant setting. A combination of longitudinal, dimensionality reduction and categorical analysis of the transcriptome from 111 liver biopsy specimens taken from 57 HCV-infected patients over time identified a molecular signature of gene expression of patients at risk of developing severe fibrosis. Significantly, alterations in gene expression occur before histologic evidence of liver disease progression, suggesting that events that occur during the acute phase of infection influence patient outcome. Additionally, a common precursor state for different severe clinical outcomes was identified. Conclusion: Based on this patient cohort, incidence of severe liver disease is a process initiated early during HCV infection of the donor organ. The probable cellular network at the basis of the initial transition to severe liver disease was identified and characterized. (HEPATOLOGY 2012;56:17–27)
Liver failure resulting from chronic hepatitis C virus (HCV) infection is the leading cause for orthotopic liver transplantation (OLT) in North America. Recurrent infection of the graft is universal in HCV patients after transplant, and in a subset of patients, the time of progression to severe fibrosis, eventual cirrhosis, and end-stage liver disease is greatly accelerated.1 Currently, the only available recourse to patients with decompensated cirrhosis is retransplantation, which is both difficult for the patient and further depletes the limited supply of available donor organs. HCV patients undergoing retransplantation as a result of decompensated cirrhosis also have a lower graft-survival rate than patients undergoing retransplantation for other indications.2
The present standard for monitoring HCV recurrence and fibrosis progression relies on histopathological examination of core needle liver biopsies. This procedure is associated with significant morbidity and frequently results in misdiagnoses of fibrosis progression because of the small size of the biopsy relative to the liver and the subjective nature of interpretation. Attempts to develop less-invasive means of diagnosing hepatic fibrosis have not proven reliably accurate thus far, although such a method is highly desirable.
Previous studies demonstrated that distinct patterns of host gene expression are associated with different clinical outcomes in HCV transplant patients.3-5 However, these studies examined differential gene expression using standard analysis methodology. We applied mathematical modeling techniques to assess transcriptional dynamics contributing to severe liver disease temporally during HCV recurrence. We utilized a combination of longitudinal topographic profiling and singular value decomposition-initiated multidimensional scaling (SVD-MDS) to identify genes involved in the progression to advanced hepatic fibrosis.
Materials and Methods
- Top of page
- Materials and Methods
- Supporting Information
Additional detail regarding methods can be found in the Supporting Materials and Methods.
Human Liver Tissue Samples.
Core needle liver biopsies were obtained from liver transplant patients at the University of Washington Medical Center (UWMC; Seattle, WA). All patients provided informed consent according to protocols approved by the Human Subject Review Committee at the University of Washington. No donor organs were obtained from executed prisoners or other institutionalized persons.
Data Processing and Normalization for SVD-MDS Analysis.
Identification of Statistically Significantly Differentially Expressed Genes.
Differentially expressed genes have been identified using a fold-change–based z-test statistic (with a fold-change parameter of 1.2; P < 0.01).
SVD-MDS Dimensionality Reduction and Derived Representations.
SVD-MDS dimensionality reduction and subsequent two-dimensional (2D) representations were obtained using the SVD-MDS method.6 Kruskal stress represents information loss resulting from dimensionality reduction/representation as a fraction of total information. The geometric objects (i.e., transcriptomic data for individual genes in different samples at different times) are nonlinearly deformed (i.e., MDS), rotated into the principal nonlinear dimensions (i.e., SVD), and then projected onto the plane. Therefore, the 2D representation captures features of the geometric objects that would otherwise only be visible in a space of higher dimension. Because the nonlinearity is not uniform, this space of higher dimension is not exactly defined, but typically corresponds to a space of two to four dimensions higher than that of the visual representation. SVD-MDS performs better than hierarchical clustering in this setting because it accounts for several of the principal dimensions of the data.
Longitudinal Time-Series Analysis.
Longitudinal analysis was achieved using the same methodology as employed previously.7 Briefly, Kohonen's maps-based classifier tests the association of any given gene with 13 topographic profiles. The profiles correspond to increasing regulatory complexity, and subsequent profiles are modeled using increasing numbers of events.
Data were warehoused in a Labkey system (Labkey, Inc., Seattle, WA). Primary data are available in accord with proposed Minimum Information About a Microarray Experiment standards (http://viromics.washington.edu). Also, data are available from the Metadata for Architectural Contents in Europe database (http://mace.ihes.fr) using an accession number (2491581318).
- Top of page
- Materials and Methods
- Supporting Information
Clinical Data and Sample Grouping.
We identified 57 chronic HCV patients undergoing OLT at the UWMC and obtained core needle biopsies from various time points post-OLT (Fig. 1). We grouped patients by post-OLT clinical outcome. Of 57 patients, 14 (25%) developed an adverse clinical outcome post-OLT (Table 1). After classifying our control, uninfected normal pool (UNP) of liver tissue, as group 1 (G1), we designated 43 HCV patients with no adverse clinical outcome as group 2 (G2). Three adverse clinical outcomes were defined for patient grouping. We first determined the patients' most recent Batts-Ludwig stage of hepatic fibrosis by having one pathologist stage the most recent biopsy before June 1, 2009, when we stopped collecting clinical information on the cohort for this study. We identified 4 patients with most recent biopsies at stage 3-4 and designated them as group 3 (G3). We also determined whether patients presented clinical symptoms of cirrhosis (e.g., portal hypertension, encephalopathy, ascites, and bleeding esophageal varices) and identified 3 patients, designated as group 4 (G4). Finally, we identified 7 HCV patients who died or underwent retransplantation resulting from graft failure, designated as group 5 (G5). All patients in G4 and G5 also developed stage 3-4 fibrosis before clinical cirrhosis or death/retransplantation. We confirmed that no patients demonstrated evidence of stage 3-4 fibrosis or symptoms of cirrhosis at the time the samples were collected. Therefore, gene-expression changes determined by our analysis to be significantly associated with severe liver injury were identified from samples taken before clinical or histological evidence of disease progression. We also divided the 111 liver biopsy specimens based on time post-OLT sampling (Fig. 1A).
|Characterization||Stage 0-2 G2 (n = 43)||Stage 3-4 G345 (n = 14)|
|Patient age (range)||59 (45-78)||62 (54-75)|
|% male patients||86.1||64.3|
|Donor age (range)||31 (10-59)||41 (13-60)|
|% male donors||78.1||64.3|
|Months post-OLT since 6/1/2009||79.6 ± 25.1||54.9 ± 18.0|
|MELD score at time of transplant||16.9 ± 8.2||14.6 ± 4.1|
|% history of ethanol abuse||72.1||21.4|
|Incidence of acute cellular rejection (%)||18.6||57.1|
Decomposition of Transcriptome Dynamics Into Functional Stages.
The relative heterogeneity of both timing of post-transplant biopsies from patients of this cohort and pathological phenotypes displayed by the different patients in the cohort (Fig. 1A) required particular attention during data analysis. Clinical annotation defined disease categories (G2-G5), and samples were further subdivided into time categories (i.e., early, intermediate, or late). These intervals were based on HCV reinfection kinetics and spreading in the donor organ and homogeneity of sample distribution. Nonprogressors (G2) also encompassed samples beyond the 2-year follow-up period of the severe liver disease groups (Fig. 1B).
We devised three analysis protocols for the transcriptomic data using different methodologies. First, we compared combined patient groups G3-G5 versus the entire G2 dataset for the early, intermediate, and late time categories separately and combined using the recently developed SVD-MDS method6 to assess the prognostic value of the gene signatures generated with the two strategies and decompose these signatures into individual gene contributions. We also performed this comparison using time-matched G2 samples. Second, we performed longitudinal topographic profiling using a previously employed7 self-organizing, maps-based classifier to investigate transcriptional dynamics within each of the three severe disease patient groups (G3-G5) and to also establish averaged gene-expression profiles for the combined G3-G5 patient groups (Fig. 1B). Finally, we used modified k-means clustering to identify a common precursor molecular signature distinguishing progression to severe fibrosis, and this transition occurred at early to intermediate time points post-OLT.
Early Transcriptome Dynamics Determine Severe Liver Disease After Transplantation in HCV-Infected Patients.
Single-linkage hierarchical clustering, based on Euclidean distances averaged over the entire microarray data set, did not reveal an apparent structure of the entire set of samples (Fig. 2A). Despite the variety of clinical phenotypes from asymptomatic to death, the overall profiles were not indicative of outcome. Time-specific profiling of the combined G345 patient groups using the early time category (G345e), as compared to the entire G2 dataset, however, identified almost 400 statistically significant differentially expressed genes (DEGs; P < 0.01; Fig. 2C; Supporting Table 1). The vast majority of these genes were down-regulated, compared to G2 expression.
Using Ingenuity Pathway Analysis (IPA), we performed functional analysis of these early DEGs associated with progression to severe fibrosis. We found that 130 of these genes were associated with inflammatory disorders and infectious disease, including numerous human leukocyte antigen (HLA) genes (e.g., HLA-DMB, HLA-DPA1, HLA-DPB1, HLA-DQB1, HLA-DRA, HLA-DRB5, HLA-E, and HLA-G). Repression of antigen presentation is expected in a post-OLT cohort, because this is the goal of the immunosuppression regimens intended to prevent graft rejection. However, these were more repressed in G345, compared to G2, patients, as were other key immune and inflammatory genes, such as immunoglobulins, Fc receptors, complement components, key signal transducers and transcriptional regulators, interferon-stimulated genes (ISGs), protein modifiers, such as ubiquitin, small ubiquitin-like modifier 2, and ISG15, proteasomal subunits, chemokines, cathepsins, and serine proteases.
Additionally, we observed that 126 molecules functionally associated with cancer were strongly repressed in G345 patient samples, compared to G2, including mediators of cell-cycle arrest and DNA-damage checkpoint control and apoptosis, indicating repressed cell-cycle control and inhibition of apoptosis. This is consistent with studies indicating that cell-cycle arrest occurs to facilitate viral replication in infected hepatocytes, and that apoptotic processes are disrupted by HCV infection.8, 9 Finally, we observed 70 molecules associated with metabolic functions, including lipid, vitamin and mineral, and cholesterol metabolism. Genes involved in multiple lipid biosynthetic processes, as well as those functioning in the synthesis and transport of membrane phospholipids and cholesterol and fatty acid biosynthesis, were also repressed in G345e biopsies. Together, these data suggest that during the first 3 months of HCV recurrence post-OLT, patients who eventually develop progressive HCV-induced liver disease experience more profound hepatic immunosuppression than G2 patients while undergoing dramatic reprogramming of mitotic and metabolic functions characterized by repression of checkpoint regulators, cell-cycle progression, and lipid biosynthesis and transport.
This initial repression was followed by the general activation of gene expression during the intermediate stages post-transplantation, as revealed by the G345m versus G2 comparison (Fig. 2D), including many DEGs related to cell cycle, cell death, and cancer. This contrasts with the G345l versus G2 comparison, which revealed an increasingly restricted pattern of gene regulation (<200 DEGs; Fig. 2E), again primarily composed of reduced expression. Because these different effects partially cancel themselves out, in the combined G345eml versus G2 profile, a limited set of DEGs equally distributed between induced and repressed phenotypes was observed (Fig. 2F). These further revealed distinct phases of transcriptome dynamics in severe liver disease patients, compared to patients without evidence of progressive disease. Early down-regulation of many genes related to inflammation, cell-cycle regulation, and lipid metabolism was followed by an intermediate activation of another subset and, finally, down-modulation of the overall transcriptional response, but increased expression of fibrogenic genes, such as type 1 collagens (e.g., COL1A1 and COL1A2), and markers of hepatic stellate cell (HSC) activation, such as secreted phosphoprotein 1/osteopontin and galectin 3 (LGALS3). Activated HSCs are the primary cellular mediators of COL and extracellular matrix deposition in HCV-induced fibrogenesis.10, 11 The temporal decrease in the number of DEGs indicates increasing heterogeneity of gene-expression patterns, leading to fewer statistically significant changes in gene expression. Heterogeneity in molecular profiles is consistent with increased heterogeneity of phenotypes in individual patients.
To assess the prognostic value of the different DEGs identified here, we employed SVD-MDS, a method of nonlinear dimensionality reduction for visualizing large datasets with many features, such as microarray data. SVD-MDS reduces data to a matrix of Euclidean distances between features and generates a lower dimensional representation while maximally preserving interfeature distances. Generally, nonlinear dimensionality reduction methods such as SVD-MDS depict an additional three to four dimensions in a visualization. Therefore, though the hierarchical clustering shown in Fig. 2A only shows the first dimension of the biological condition space, representations shown in Fig. 2B and 2G-2J visually represent approximately the first five dimensions, thereby more faithfully addressing the structure of the data. This method allows data comparison between patients with different outcomes, as well as defining, among statistically significant DEGs, those contributing most to distinguishing G345 progressors from G2 nonprogressors. Generally, the more distant the groups and the closer the patient samples are within each group, the better the prognostic value of any given signature.
Hierarchical clustering of the entire set of genes did not clearly separate the samples into patient groups (Fig. 2A,B). However, the DEG G345e versus G2 (Fig. 2G), G345m versus G2 (Fig. 2H), and G345l versus G2 (Fig. 2I) improved separation of the liver transplant patients from the UNP G1 control group and, concomitantly, provide fewer distinctions between G2 and G345. This behavior is concordant with the time-specific analysis discussed above and is echoed by the G345eml versus G2 DEG (Fig. 2J). Therefore, DEGs associated with severe disease were harder to detect over time, indicating that early events play a decisive role in the development of severe liver disease and lead to a variety of observable phenotypes at later stages. Importantly, SVD-MDS analysis also revealed that both G2 and G345 patient groups increasingly differentiated from the G1 UNP controls, which represent pooled healthy liver gene-expression profiles. This indicates a slow evolution to more heterogeneous gene expression, regardless of clinical outcome. Though the nature of this evolution is somewhat unclear, this poses important questions regarding the stochasticity of liver disease progression kinetics and suggests that decisive early transcriptional repression of select inflammatory mediators, cell-cycle regulators, and genes involved in both lipid biogenesis and catabolism predict disease progression.
We also directly compared time-matched G2 and G345 samples. Consistent with the first analysis, clustering analysis showed that gene expression alone was insufficient to segregate patients according to clinical outcome (Supporting Fig. 1). These DEGs were similarly repressed and were functionally consistent with significant DEGs identified in the first analysis. These results thus confirm that early events post-OLT are detrimental to liver physiology. Note that we refrained from providing direct G2 versus G3 or G4 or G5 comparisons, because the amount of available biopsies in this cohort was too small to provide for robust insights. Such direct comparisons will become feasible in the analysis of a larger independent cohort or in meta-analysis across different cohorts.
Gene-Expression Profiles Associated With Liver Disease Progression Over Time.
To better understand how early events lead to severe liver disease and to compare differences in gene-expression patterns over time within each individual disease group, we performed a longitudinal kinetic analysis. We used a recently utilized classifier7 derived from the analysis of many different longitudinal, publicly available and in-house datasets using Kohonen maps approach, which fits gene expression to topographic maps representing distinct regulatory patterns. Our classifier comprised relevant topographic groups: g1- 6 for initial positive regulation; a neutral g0 group for genes expressed but unchanged; and the mirror g-1-g-6 groups for negative regulation (Fig. 3A). The classifier tests for statistical significance (i.e., fold-change–based z test) of association with individual topographic groups by testing the statistical significance of the logarithmic fold-change difference in expression of every individual gene at every time point against its estimated baseline, with absolute expression change rescaled to unity. Thus, gene expression was analyzed for its characteristic, statistically significant “shape” over time, rather than magnitude of change. We further subdivided the time categories to generate a fourth category (Fig. 1B). Because we were mainly interested in identifying genes involved in severe liver disease development, we focused on genes that permanently change expression over time (Fig. 3B; Supporting Table 2).
Using IPA, we categorized 48 genes related to inflammatory responses and immune cell trafficking, particularly phagocyte and lymphocyte recruitment and chemotaxis, including many C-X-C and C-C chemokines and chemokine receptors. Also, we observed molecules bridging innate and adaptive immune functions, including signal transduction and activation of immune and inflammatory transcriptional responses, proinflammatory cytokines, Fc receptors, complement components, ISGs, HLA alleles, and lymphocyte activation. We also identified increases in genes associated with HSC activation and COL deposition, including TIMP metalloproteinase inhibitor, LGALS3, and multiple COL transcripts. Finally, 59 genes associated with cancer also gradually increased, including many associated with metastasis, cell proliferation, and cell death, indicating that dysregulation of normal cell division and apoptotic mechanisms underlie hepatic inflammation and COL deposition.
We also evaluated the functional significance of DEG down-regulated over time after OLT. We identified 12 genes associated with lipid, drug, vitamin and mineral, and carbohydrate metabolism. These are involved in lipid biosynthesis, fatty acid oxidation, and amino acid and glucose metabolism. G345 patients therefore demonstrated reduced hepatic metabolic function, consistent with reductions in metabolic activity previously observed at late time points in HCV-infected hepatic cells in vitro.12 Additionally, we identified 11 genes associated with cancer, specifically those involved in cell-cycle control, such as retinoblastoma-like 2 and cyclin-dependent kinase inhibitor 3 (CDKN3), and regulators of cellular differentiation. Because cancer-related genes associated with cellular proliferation steadily increased, those associated with cell-cycle checkpoint control and cell-type specification were down-regulated. This indicates that patients with progressive liver disease experience a loss of differentiation and checkpoint cell-cycle arrest, consistent with the concordant gradual increase in proliferative capacity. This also suggests a mechanism by which chronic HCV infection contributes to tumorigenesis of hepatocellular carcinoma (HCC).
Gene Signature Characterizing a Precursor State for Severe Liver Disease.
The SVD-MDS method used in the analysis presented in Fig. 2G-J and Supporting Fig. 1G-J allows the computation of two additional parameters aside from Kruskal stress (i.e., information loss during dimensionality reduction): external isolation (i.e., the arithmetic average intergroup distance) and internal cohesiveness (i.e., the intragroup distance). Both parameters determined for the analyses peak 3-6 months post-OLT (Fig. 4A), indicating that the signatures derived from these time categories generate the relative maximal resolution. Hence, the early stages of HCV reinfection best characterize overall clinical outcome. We then used the time-specific analysis to define a gene-expression pattern-based distance measure between any of the individual groups and with combined G2345 and G345, as well as G45 longitudinal analysis. To investigate severe liver disease progression according to time and patient outcome, these measures were then subjected to k-means clustering13 using intergroup distances as additional constraints. This analysis indicates the existence of a common precursor state (G345) for all progressor groups (Fig. 4B, red), from which all three adverse outcomes split individually. This precursor state is comprised of 35 DEGs (Table 2), which distinguish the transformation to a progressive disease outcome long before histological or clinical evidence of severe disease.
|Accession Number||Symbol||Entrez Gene Name||Location||Molecule Type|
|NM_018849||ABCB4||ATP-binding cassette, subfamily B (MDR/TAP), member 4||PM||Transporter|
|NM_005891||ACAT2||Acetyl-CoA acetyltransferase 2||Cytoplasm||Enzyme|
|NM_001005386||ACTR2||ARP2 actin-related protein 2 homolog (yeast)||PM||Other|
|NM_018840||C20orf24||Chromosome 20 open reading frame 24||Cytoplasm||Other|
|NM_020199||C5orf15||Chromosome 5 open reading frame 15||unknown||Other|
|NM_001554||CYR61||Cysteine-rich, angiogenic inducer, 61||ECS||Other|
|NM_001946||DUSP6||Dual-specificity phosphatase 6||Cytoplasm||Phosphatase|
|NM_001994||F13B||Coagulation factor XIII, B polypeptide||Cytoplasm||Enzyme|
|NM_013402||FADS1||Fatty acid desaturase 1||PM||Enzyme|
|NM_014923||FNDC3A||Fibronectin type III domain containing 3A||Cytoplasm||Other|
|NM_002116||HLA-A||Major histocompatibility complex, class I, A||PM||TM receptor|
|U88244||HLA-G||Major histocompatibility complex, class I, G||PM||TM receptor|
|NM_015525||IBTK||Inhibitor of Bruton agammaglobulinemia tyrosine kinase||Cytoplasm||Other|
|NM_002216||ITIH2||Interalpha (globulin) inhibitor H2||ECS||Other|
|AB067508||KLHL29||Kelch-like 29 (Drosophila)||unknown||Other|
|NM_002294||LAMP2||Lysosomal-associated membrane protein 2||PM||Enzyme|
|NM_002404||MFAP4||Microfibrillar-associated protein 4||ECS||Other|
|NM_002408||MGAT2||Mannosyl (alpha-1,6-)-glycoprotein beta-1,2-N-acetylglucosaminyltransferase||Cytoplasm||Enzyme|
|NM_021129||PPA1||Pyrophosphatase (inorganic) 1||Cytoplasm||Enzyme|
|NM_015216||PPIP5K2||Diphosphoinositol pentakisphosphate kinase 2||Cytoplasm||Other|
|NM_014624||S100A6||S100 calcium-binding protein A6||Cytoplasm||Transporter|
|NM_006918||SC5DL||Sterol-C5-desaturase (ERG3 delta-5-desaturase homolog, Saccharomyces cerevisiae)-like||Cytoplasm||Enzyme|
|NM_019844||SLCO1B3||Solute carrier organic anion transporter family, member 1B3||PM||Transporter|
|NM_001060||TBXA2R||Thromboxane A2 receptor||PM||GPCR|
|NM_004617||TM4SF4||Transmembrane 4 L six family member 4||PM||Other|
|NM_006074||TRIM22||Tripartite motif-containing 22||Cytoplasm||Transcription regulator|
|NM_006357||UBE2E3||Ubiquitin-conjugating enzyme E2E 3 (UBC4/5 homolog, yeast)||Nucleus||Enzyme|
In the absence of time-resolved samples from healthy, non-HCV patients, we were not able to determine whether a common G2345 (Fig. 4B, black) state exists or how this hypothetical intermediate state would relate to G2 and G345. More important, the predicted common G345 precursor state confirmed our observation that eventual severe liver disease is programmed early post-OLT, and in combination with the time-specific analyses described above, identified DEGs distinguishing progressors and nonprogressors within 6 months of transplantation.
Using IPA, we generated a network of directly interacting molecules based on the network analysis of the transitional signature and the G345e time-specific gene sets (Fig. 4C and Supporting Fig. 2). We confirmed that repression of genes involved in cell-cycle regulation and stress responses (e.g., cyclin D1 and X-box-binding protein), innate immunity (e.g., signal transducer and activator of transcription 1), and antigen presentation (e.g., HLA-A, HLA-G, and HLA-E) characterize transition to a progressive phenotype. Additionally, COL up-regulation was detected within several months of transplantation, which is months or years before fibrosis is histologically detectable. Coupled with our finding that the statistically significant up-regulation of COL expression correlates with disease progression over time, this indicates that COL transcription is both critical to the mechanism of fibrogenesis and potentially useful as a predictive marker to identify patients at risk of HCV-induced liver disease before extensive COL deposition and associated liver damage.
- Top of page
- Materials and Methods
- Supporting Information
Investigating the influence of transcriptional profiles on clinical outcome in patients after transplantation could lead to more refined prognostic models. This study represents the first in which SVD-MDS analysis has been used to identify contributions of significant DEG associated with HCV-induced liver disease progression. The SVD-MDS method reduced dimensionality by removing dimensions with little information (i.e., high biological noise) and by emphasizing the main contributing dimensions. This is a significant advantage over clustering techniques, which here failed to provide meaningful biological insight. In this context, SVD-MDS demonstrates that pertinent information contained in the entire set of measured transcript abundances is enriched during the statistical analysis. The unique molecular profiles that distinguish patients who develop severe liver disease provide insight into the biological mechanism of disease progression, both before the advent of disease and over time. Furthermore, they provide a basis for larger validation studies or meta-analysis across additional different cohorts of HCV patients in future efforts to establish definite molecular correlates.
Our transitional signature suggests that the key regulators of a precursor state leading to progression play the most critical role at early to intermediate time points post-OLT. Patients who eventually develop the most severe liver disease may be most clearly distinguished by DEG within 3 months post-OLT, compared to patients who do not progress. Specifically, we observed a broad repression of genes related to antigen presentation, immune responses, and cell-cycle regulation in patients who progress. This suggests that long-term clinical outcome is determined by early reprogramming of the donor liver during recurrence and, specifically, by blunting responses that prevent unchecked inflammation and cell division. These processes are directly connected to hallmarks of HCV-induced hepatic disease, such as chronic inflammatory hepatitis, cirrhosis, and HCC.
Although down-regulation of immune transcripts is expected in patients taking immunosuppressant drugs to prevent allograft rejection, in patients who developed severe disease, we observed a profound repression of genes related to antiviral responses. This distinguishes this signature with regard to long-term outcome, compared to the clinical situation early post-OLT. Acute cellular rejection (ACR) is difficult to distinguish from HCV recurrence, based on analysis of patient liver biopsies, as a result of common histological features. Previous studies comparing HCV patients with and without ACR demonstrate that many of the repressed genes are significantly up-regulated during ACR in HCV patients.3, 14 Additionally, repression of innate and inflammatory genes was characteristic of HCV recurrence, rather than ACR, in HCV transplant patients.15 This indicates that though short-term clinical factors, such as ACR, may confound long-term efforts to develop molecular signatures of liver disease pathogenesis, repression of these innate immune genes is more widespread and of greater magnitude.
Immune repression early in infection may contribute to increased hepatocyte infection during HCV recurrence and thus may create a more favorable environment for progression to severe disease. Although antigen presentation has been associated with HCV pathogenesis,16-18 these pathways are normally suppressed by allograft rejection drugs, causing impaired T-cell responses in HCV transplant patients. However, it is difficult to determine the effect of specific immunosuppressive regimens, because patients are routinely treated with different drugs and dosing regimens. Generally, the immunosuppressive regimens used are less likely to repress innate immune responses that could attenuate the severity of HCV recurrence. Innate immune antagonism by HCV infection may result in the virus eliciting a transcriptional program that eventually results in fibrosis and disease progression, which is partially reflected by the increase in inflammatory genes over time caused by infiltrating leukocytes.
HCV facilitates its replication by antagonizing the induction of antiviral interferons, ISGs, and antiviral cytokines through the action of the viral nonstructural protein (NS)3/4 protease and NS5A.19-22 Clinicians have not routinely treated HCV patients with post-OLT ribavirin and pegylated interferon, primarily because the high expense and harsh side effects of this treatment regimen do not justify its use in patients recovering from organ transplantation. However, a recent study demonstrated that post-OLT treatment resulted in stable or improved fibrosis scores, even in some patients who did not demonstrate sustained virologic response.23 Our data indicating that repressed antiviral gene expression early in infection determines transition to severe disease suggests that patients may benefit from early therapeutic intervention during HCV recurrence to boost innate immune genes not effected by immunosuppressant drugs during the first 3 months post-OLT.
Early repression of cell-division mediators in patients who progress also indicates that these transcriptional profiles are altered. These genes are largely involved in controlling cell-cycle progression and transcriptional activation of related pathways, and their repression suggests the induction of cell-cycle arrest. In vitro, HCV infection induces cell-cycle arrest early in infection to facilitate viral replication.8, 24, 25 The repression of genes promoting cell-cycle progression may represent a greater number of infected hepatocytes, suggesting that more severe cases of recurrence facilitate a hepatic environment that augments further cell-cycle dysregulation. As our kinetic analysis indicates, cell-cycle regulators decrease over time in patients who progress, whereas genes promoting cell division, such as growth factors, continue to increase. One of these genes, CDKN3, regulates specific cell-cycle networks related to HCV-induced cirrhosis and HCC,26 supporting the notion that early cell-cycle arrest occurring in infected hepatocytes can result in the loss of key regulatory functions over time and can promote eventual tumorigenesis. Additional repression of genes such as breast cancer 1, early onset (BRCA1), which are critical mediators of DNA damage repair, may result in genetic lesions that also contribute to cell death and eventual oncogenesis, as is the case for the BRCA1-interacting gene, brain and reproductive organ expressed, which promotes HCC growth.27 Increasingly altered cell-cycle regulation contributes to the altered mitotic state created by the initial repression of cell-cycle regulators early in infection and, ultimately, leads to cell death, aberrant proliferation, and, potentially, cancer.
Our analysis demonstrates the dynamic transcriptional response elicited by HCV in the post-transplant setting. Early repression of innate immunity and cell-cycle progression may establish a state in the donor organ facilitating viral replication and the establishment of more widespread chronic infection. Also contributing to this is the increasing presence of collagens and other fibrogenic transcripts. After 3 months post-OLT, this hepatic reprogramming mediates the transition to progressive disease, characterized by gradual increases in DEG associated with inflammation, HSC activation, COL deposition, cell proliferation, and cell death, and decreases in genes related to cell-cycle control. Our study identifies a signature early during recurrence consistent with early cellular responses to HCV infection distinguishing progressors in the post-transplant setting. This yields insight into the earliest host responses to HCV recurrence and raises the exciting possibility of identifying and treating patients, based on transcriptional profiling, long before disease progression or significant damage to the donor organ.
- Top of page
- Materials and Methods
- Supporting Information
The authors thank James Perkins and Renuka Bhattacharya for their clinical support.
- Top of page
- Materials and Methods
- Supporting Information
- 26Cyclin-dependent kinase inhibitor 3 (CDKN3) novel cell cycle computational network between human non-malignancy associated hepatitis/cirrhosis and hepatocellular carcinoma (HCC) transformation. Cell Prolif 2011; 44: 291-299., , , .
- 28Limma: linear models for microarray data. In: Gentleman R, Carey V, Dudoit S, Irizarry R, Huber W, ed. Bioinformatics and Computational Biology Solutions Using R and Bioconductor. New York: Springer; 2005: 397-420..
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- Materials and Methods
- Supporting Information
Additional Supporting Information may be found in the online version of this article.
|HEP_25612_sm_SuppFig1.eps||1673K||Supporting Information Figure 1.|
|HEP_25612_sm_SuppFig2.eps||4179K||Supporting Information Figure 2.|
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