Article first published online: 2 JUL 2012
Copyright © 2012 American Association for the Study of Liver Diseases
Volume 56, Issue 2, pages 484–491, August 2012
How to Cite
Liu, S., McCormick, K. D., Zhao, W., Zhao, T., Fan, D. and Wang, T. (2012), Human apolipoprotein E peptides inhibit hepatitis C virus entry by blocking virus binding. Hepatology, 56: 484–491. doi: 10.1002/hep.25665
Potential conflict of interest: Nothing to report.
Supported by the National Institutes of Health (NIH), grants NIH R21AI083389 (to T.W.); NIH R01DK088787 (to T.W.); NIH R21HL106325 (to D.F.).
- Issue published online: 25 JUL 2012
- Article first published online: 2 JUL 2012
- Accepted manuscript online: 15 FEB 2012 04:10AM EST
- Manuscript Accepted: 9 FEB 2012
- Manuscript Received: 6 SEP 2011
Additional Supporting Information may be found in the online version of this article.
|HEP_25665_sm_SuppFig1.tif||11189K||Supporting Information Figure 1. Mapping the anti-viral activity of hEP. In (A) and (B), HCVcc-luc was first mixed with indicated peptides (20 μg/ml, 2.7 μM) and then incubated with Huh7.5.1 cells for two hours at 37°C to initiate infection. After additional 48 hours incubation, luciferase activity was measured. Results are presented as fold of inhibition relative to infections containing DMSO (n=5, mean ± sd). (C) 20 μg of hEP-2 or hEP-2/ΔCys were resuspended in PBS and kept at room temperature for 30minutes. Peptides were then mixed with 2X SDS-sample buffer without DTT and boiled for 5 minutes. Alternatively, samples were simply left unboiled or mixed with sample buffer containing DTT (2 mM). Peptide samples were then subjected to electrophoresis on a 16% tricine non-reducing gel (Invitrogen). The calculated molecular weight of hEP-2 is 4034.82 Da. (D) and (E) Mass spectrometric analysis of hEP-2 or hEP-2/ΔCys. Notice the dimer peak of hEP-2 in (D).|
|HEP_25665_sm_SuppFig2.tif||131K||Supporting Information Figure 2. hEP-2 inhibits HCVcc binding to Huh7.5.1 cells. The effects of hEP-2 (2.7 μM) or scrambled peptide on HCV binding were determined as described in Fig. 5D. Results were calculated as relative RNA copies with numbers obtained from the scrambled peptide treated wells set to 100 (mean of n=3; error bars, s.d.). ** p<0.005.|
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