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Additional Supporting Information may be found in the online version of this article.

FilenameFormatSizeDescription
HEP_25742_sm_SuppFig1.tif5768KSupporting Information Figure 1. Immunohistochemistry for adiponectin in normal and steatotic livers (pediatric non-alcoholic fatty liver disease [pNAFLD]). This image provides additional information about adiponectin positivity in endothelial cells and steatotic hepatocytes. In normal and steatotic livers, adiponectin is also expressed by the endothelial cells (arrows) of the hepatic artery branches (A) and hepatic sinusoids (B). In steatotic livers, hepatocytes with steatosis express adiponectin (arrows in C).
HEP_25742_sm_SuppFig2.tif4081KSupporting Information Figure 2. Figure S2. Separate channels of double immunofluorescence for cytokeratin-7 (CK-7; green) and adiponectin (red) in pNAFLD. Nuclei were counterstained with 4′,6-diamidino-2-phenylindole (DAPI) and are shown in blue. As indicated by the white arrows, the cells (fibroblasts or hepatocytes) around reactive ductules are clearly negative for both antigens, confirming that the positivity is specifically restricted to hepatic progenitor cells (HPCs; yellow arrows) in reactive ductules. Notably, the positivity in several HPCs is localized to an inner position (see the arrows in the separate channels), in which 2 CK-7-positive HPCs face each other; this aspect further supports the evidence that the CK7-positive HPCs are truly positive for this adipokine and excludes the possibility that the positivity derives from the bleeding of color from closely adjacent cells into HPCs.
HEP_25742_sm_SuppFig3.tif5047KSupporting Information Figure 3. Figure S3. Separate channels of double immunofluorescence for CK-7 (green) and resistin (red) in pNAFLD. Nuclei were counterstained with DAPI and are shown in blue. As indicated by the white arrows, the cells (fibroblasts or hepatocytes) around reactive ductules are clearly negative for both antigens, confirming that the positivity is specifically restricted to HPCs (yellow arrows) in reactive ductules. Notably, the positivity in several HPCs is localized to an inner position (see the arrows in the separate channels), in which 2 CK7-positive HPCs face each other; this aspect further supports the evidence that the CK7-positive HPCs are truly positive for this adipokine and excludes the possibility that the positivity derives from the bleeding of color from closely adjacent cells into HPCs.
HEP_25742_sm_SuppFig4.tif5654KSupporting Information Figure 4. Figure S4. Separate channels of double Immunofluorescence for CK-7 (green) and glucagon-like peptide 1 (GLP-1; red) in pNAFLD. Nuclei were counterstained with DAPI and are shown in blue. As indicated by the white arrows, the cells (fibroblasts or hepatocytes) around reactive ductules are clearly negative for both antigens, confirming that the positivity is specifically restricted to HPCs (yellow arrows) in reactive ductules. Notably, the positivity in several HPCs is localized to an inner position (see the arrows in the separate channels), in which 2 CK7-positive HPCs face each other; this aspect further supports the evidence that the CK7-positive HPCs are truly positive for this adipokine and excludes the possibility that the positivity derives from the bleeding of color from closely adjacent cells into HPCs.
HEP_25742_sm_SuppInfo.doc32KSupporting Information

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