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Additional Supporting Information may be found in the online version of this article.

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HEP_25763_sm_SuppFig1.tif170KSupplementary Figure 1. Anti-HCV effects and cytotoxicity of 25(OH)D3 at high concentrations. HuH-7 cells were treated with indicated concentrations of 25(OH)D3 followed by inoculation with HCVcc. HCV production was assessed by measuring the HCV core Ag (A). HCV core Ag levels in culture medium and cell lysates are indicated by open and closed bars respectively. Cell viability was also assessed by the WST-8 assay after treatment with indicated concentrations of 25(OH)D3 (B). Results are shown as means ± SD of the percent of the control.
HEP_25763_sm_SuppFig2.tif183KSupplementary Figure 2. ISG induction by 25(OH)D3 treatment. To assess the effects of 25(OH)D3 on IFN signaling, the expression of MxA (A) and OAS (B) mRNA with or without interferon treatment were measured by TaqMan Gene Expression Assay. Results are shown as means log fold increase ± SD.
HEP_25763_sm_SuppFig3.tif176KSupplementary Figure 3. Anti-HCV effects of 25(OH)D3 in multiple HCV strains other than JFH-1. Anti-HCV effects of 25(OH)D3 on J6/JFH-1, H77S.2, and JFH-1/V2440L. Infectivity titers in culture medium and cell lysates were measured after a 3-day treatment. Results are shown as means ± SD of the percentage of untreated. *p < 0.05 compared with untreated.
HEP_25763_sm_SuppFig4.tif207KSupporting Information Figure 4. Supplementary Figure 4. Production and anti-HCV effect of 1,25(OH)2D3 in HuH-7 cells. (A) Minimal conversion of 25(OH)D3 to 1,25(OH)2D3 in HuH-7 cells. HuH-7 cells were treated with 25(OH)D3 at various concentrations (0.5, 1.0, 2.0 and 5.0 μM), and production of 1,25(OH)2D3 was measured in the medium. Results are shown as means ± SD. (B) No anti-HCV effect of 1,25(OH)2D3 at low concentrations. Treatment with 1,25(OH)2D3 at concentrations of 1.0 to 0.001 μM, causes no effects on HCV core Ag levels measured in culture medium (open bar) and cell lysates (closed bar). Results are shown as means ± SD of the percentage of ethanol.
HEP_25763_sm_SuppTab1.doc36KSupporting Information Table 1.
HEP_25763_sm_SuppTab2.doc32KSupporting Information Table 2.
HEP_25763_sm_SuppInfo.doc33KSupporting Information

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