Targeted delivery of interferon-α to hepatitis B virus-infected cells using T-cell receptor-like antibodies†
Article first published online: 12 JUL 2012
Copyright © 2012 American Association for the Study of Liver Diseases
Volume 56, Issue 6, pages 2027–2038, December 2012
How to Cite
Ji, C., Sastry, K. S.R., Tiefenthaler, G., Cano, J., Tang, T., Ho, Z. Z., Teoh, D., Bohini, S., Chen, A., Sankuratri, S., Macary, P. A., Kennedy, P., Ma, H., Ries, S., Klumpp, K., Kopetzki, E. and Bertoletti, A. (2012), Targeted delivery of interferon-α to hepatitis B virus-infected cells using T-cell receptor-like antibodies. Hepatology, 56: 2027–2038. doi: 10.1002/hep.25875
Potential conflict of interest: Nothing to report.
- Issue published online: 4 DEC 2012
- Article first published online: 12 JUL 2012
- Accepted manuscript online: 9 JUN 2012 10:50AM EST
- Manuscript Accepted: 21 MAY 2012
- Manuscript Received: 22 FEB 2012
- Agency of Science Technology and Research (A*STAR-Singapore)
- Roche Singapore Translational Medicine Hub
Additional Supporting Information may be found in the online version of this article.
|HEP_25875_sm_SuppFig1.tiff||2641K||Supporting Information Figure 1. TCR-L binding ability in HBV producing HepG2 cell lines. Control HepG2 cells and HepG2 -13 (HBV producing cells) were fixed in 1%PFA, and stained with cTCR-L or sTCR-L. Alexa Fluor-647-Tyramide Signal Amplification kit was used to visualize the antibody binding (red). Nuclei were stained with DAPI (blue).|
|HEP_25875_sm_SuppFig2.tif||1593K||Supporting Information Figure 2. HepG2 cells were transfected with pISRE-Luc reporter vector, pulsed with or without 10μg/ml HBs183-91 peptide and treated with 3-fold serially diluted IFNα, sTCR-L, or sTCR-L/IFNα. Luciferase activity was measured 48h post-treatment.|
|HEP_25875_sm_SuppFig3.tiff||1572K||Supporting Information Figure 3. TCR-L/IFNα trigger CXCL-10 production on IFNγ treated Hepg2 cells. CXCL-10 production was measured by intracellular CXCL-10 in HepG2 cells. HepG-2 cells were incubated with IFNγ, not pulsed or pulsed with HBs183-91 peptide (1μM) and incubated with the indicated TCR-L overnight. Bars represented the % of CXCL-10 positive cells obtained with the different conditions. Inserted hystogram displayed mean fluorescence intensity (MFI) of the Hbs183-91 peptide pulsed target cells treated with sTCR-L/IFNα, sTCR-L or cTCR-L/IFNα.|
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