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Additional Supporting Information may be found in the online version of this article.

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HEP_25875_sm_SuppFig1.tiff2641KSupporting Information Figure 1. TCR-L binding ability in HBV producing HepG2 cell lines. Control HepG2 cells and HepG2 -13 (HBV producing cells) were fixed in 1%PFA, and stained with cTCR-L or sTCR-L. Alexa Fluor-647-Tyramide Signal Amplification kit was used to visualize the antibody binding (red). Nuclei were stained with DAPI (blue).
HEP_25875_sm_SuppFig2.tif1593KSupporting Information Figure 2. HepG2 cells were transfected with pISRE-Luc reporter vector, pulsed with or without 10μg/ml HBs183-91 peptide and treated with 3-fold serially diluted IFNα, sTCR-L, or sTCR-L/IFNα. Luciferase activity was measured 48h post-treatment.
HEP_25875_sm_SuppFig3.tiff1572KSupporting Information Figure 3. TCR-L/IFNα trigger CXCL-10 production on IFNγ treated Hepg2 cells. CXCL-10 production was measured by intracellular CXCL-10 in HepG2 cells. HepG-2 cells were incubated with IFNγ, not pulsed or pulsed with HBs183-91 peptide (1μM) and incubated with the indicated TCR-L overnight. Bars represented the % of CXCL-10 positive cells obtained with the different conditions. Inserted hystogram displayed mean fluorescence intensity (MFI) of the Hbs183-91 peptide pulsed target cells treated with sTCR-L/IFNα, sTCR-L or cTCR-L/IFNα.
HEP_25875_sm_SuppInfo.doc59KSupporting Information

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