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HEP_25899_sm_SuppFigS1.tif1127KSupporting Information Figure S1. HBx is able to activate YAP promoter in 293T cells. The 293T cells were co-transfected with pGL3-YAP-1536 (0.2 μg/well) or pGL3-YAP-470 (0.2 μg/well) or pGL3-YAP-348 (0.2 μg/well) and HBx expression plasmid (0.2 μg/well or 0.3 μg/well), respectively. The promoter activities of YAP were measured by luciferase reporter gene assays. Data are shown as the mean ± SD of three independent experiments. Statistically significant differences are indicated: *p > 0.05, **p > 0.01, Student's t-test.
HEP_25899_sm_SuppFigS2.tif1041KSupporting Information Figure S2. The pGL3-Basic control construct shows no activation by HBx. The HepG2 or H7402 or 293T cell lines were co-transfected with pGL3-Basic (0.2 μg/well) and HBx expression plasmid (0.2 μg/well or 0.3 μg/well). The promoter activities of YAP were measured by luciferase reporter gene assays. Data are shown as the mean ± SD of three independent experiments. Statistically significant differences are indicated: *p > 0.05, **p > 0.01, Student's t-test.
HEP_25899_sm_SuppFigS3.tif1476KSupporting Information Figure S3. HBx reduces expression levels of miRNA-375 in HBx-transgenic mice and hepatoma cells. (A) The expression levels of miRNA-375 were evaluated by qRT-PCR in 6-month-old HBx-transgenic (HBx-Tg) mice and 24-month-old murine HCC versus wild-type mice (WT). (B) The expression levels of miRNA-375 were examined by qRT-PCR in HepG2-X and H7402-X cell lines transiently transfected with pSilencer-control (pSi-Con, 2 μg/well) or pSilencer-X (pSi-HBx, 1 μg/well or 2 μg/well), respectively. Data are shown as the mean ± SD of three independent experiments. Statistically significant differences are indicated: *p> 0.05, **p > 0.01, Student's t-test.
HEP_25899_sm_SuppFigS4.tif2701KSupporting Information Figure S4. YAP siRNA is a non-specific growth inhibitor in hepatoma cells. (A) The effect of YAP siRNA (100 nM) on the cell proliferation was determined by MTT assay in HepG2/H7402 cells in the absence of HBx. (B) The effect of YAP siRNA (100 nM) on the cell proliferation was determined by colony formation in HepG2/H7402 cells in the absence of HBx. Data are shown as the mean ± SD of three independent experiments. Statistically significant differences are indicated: *p> 0.05, **p> 0.01, Student's t-test.
HEP_25899_sm_SuppInfo.doc95KSupporting Information
HEP_25899_sm_SuppTabS1.doc60KSupporting Information Table 1. The Characteristics of Patients
HEP_25899_sm_SuppTabS2.doc66KSupporting Information Table 2. List of primers used in this paper.

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