Additional Supporting Information may be found in the online version of this article.

HEP_25985_sm_SuppFig1.tif3660KSupporting Information Figure 1. (A) Suzuki's score showing representative picture of scoring 1, 2, 3 and 4 on H&E staining at an original magnification of x200. (B) Representative picture of WT and CD39tg donor liver stained for cleaved caspase-3 at an original magnification of x100. Sections were scored based on the percentage of central veins with surrounding cleaved caspase-3 positive cells.
HEP_25985_sm_SuppFig2.tif371KSupporting Information Figure 2. CD39 transgene expression on donor liver tissue and lymphocytes. (A) CD39 expression analysed by real-time PCR on WT and CD39tg donor livers. Bars represent means ± SEM of human CD39 gene expression relative to GAPDH (2-dCt), n=6 per group. **, p<0.01 by Student t-test. (B) Flow cytometric analysis of CD39 expression on donor lymphocytes from WT and CD39tg livers. Data represent MFI ± SEM, n=9 per group.
HEP_25985_sm_SuppFig3.tif774KSupporting Information Figure 3. T regulatory cell number and function. Treg cell proportion (A) and absolute numbers (B) were determined in the spleen of WT and CD39tg mice using CD4+/FoxP3+ gating. (B) Histograms represent mean ± SEM, n=8 per group. ns, not significant by Student t-test. (C) Histograms represent CFSE stained WT T effector cells after 72hrs coculture with WT accessory cells and WT (solid grey) or CD39tg (black line) Treg at different ratios Treg/Teff. (D) Percentage of WT and CD39tg Treg suppression on Teff proliferation at different ratios Treg/Teff. Each dot represents the percentage of suppression calculated with means of triplicates. Data are representative of three independent experiments.
HEP_25985_sm_SuppInfo.doc23KSupporting Information

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