These authors contributed equally to this work.
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Liver Injury/Regeneration
Liver grafts from CD39-overexpressing rodents are protected from ischemia reperfusion injury due to reduced numbers of resident CD4+ T cells†
Article first published online: 14 MAR 2013
DOI: 10.1002/hep.25985
Copyright © 2012 American Association for the Study of Liver Diseases
Additional Information
How to Cite
Pommey, S., Lu, B., McRae, J., Stagg, J., Hill, P., Salvaris, E., Robson, S. C., d'Apice, A. J.F., Cowan, P. J. and Dwyer, K. M. (2013), Liver grafts from CD39-overexpressing rodents are protected from ischemia reperfusion injury due to reduced numbers of resident CD4+ T cells. Hepatology, 57: 1597–1606. doi: 10.1002/hep.25985
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Potential conflict of interest: Nothing to report.
- ‡
These authors contributed equally to this work.
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Publication History
- Issue published online: 8 APR 2013
- Article first published online: 14 MAR 2013
- Accepted manuscript online: 24 JUL 2012 10:04AM EST
- Manuscript Accepted: 14 JUL 2012
- Manuscript Received: 9 FEB 2012
Funded by
- NHMRC (Australia)
- Genzyme Renal Innovations Program
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Additional Supporting Information may be found in the online version of this article.
| Filename | Format | Size | Description |
|---|---|---|---|
| HEP_25985_sm_SuppFig1.tif | 3660K | Supporting Information Figure 1. (A) Suzuki's score showing representative picture of scoring 1, 2, 3 and 4 on H&E staining at an original magnification of x200. (B) Representative picture of WT and CD39tg donor liver stained for cleaved caspase-3 at an original magnification of x100. Sections were scored based on the percentage of central veins with surrounding cleaved caspase-3 positive cells. | |
| HEP_25985_sm_SuppFig2.tif | 371K | Supporting Information Figure 2. CD39 transgene expression on donor liver tissue and lymphocytes. (A) CD39 expression analysed by real-time PCR on WT and CD39tg donor livers. Bars represent means ± SEM of human CD39 gene expression relative to GAPDH (2-dCt), n=6 per group. **, p<0.01 by Student t-test. (B) Flow cytometric analysis of CD39 expression on donor lymphocytes from WT and CD39tg livers. Data represent MFI ± SEM, n=9 per group. | |
| HEP_25985_sm_SuppFig3.tif | 774K | Supporting Information Figure 3. T regulatory cell number and function. Treg cell proportion (A) and absolute numbers (B) were determined in the spleen of WT and CD39tg mice using CD4+/FoxP3+ gating. (B) Histograms represent mean ± SEM, n=8 per group. ns, not significant by Student t-test. (C) Histograms represent CFSE stained WT T effector cells after 72hrs coculture with WT accessory cells and WT (solid grey) or CD39tg (black line) Treg at different ratios Treg/Teff. (D) Percentage of WT and CD39tg Treg suppression on Teff proliferation at different ratios Treg/Teff. Each dot represents the percentage of suppression calculated with means of triplicates. Data are representative of three independent experiments. | |
| HEP_25985_sm_SuppInfo.doc | 23K | Supporting Information |
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