Monocyte subsets in human liver disease show distinct phenotypic and functional characteristics†
Version of Record online: 4 DEC 2012
Copyright © 2012 American Association for the Study of Liver Diseases
Volume 57, Issue 1, pages 385–398, January 2013
How to Cite
Liaskou, E., Zimmermann, H. W., Li, K.-K., Oo, Y. H., Suresh, S., Stamataki, Z., Qureshi, O., Lalor, P. F., Shaw, J., Syn, W.-k., Curbishley, S. M. and Adams, D. H. (2013), Monocyte subsets in human liver disease show distinct phenotypic and functional characteristics. Hepatology, 57: 385–398. doi: 10.1002/hep.26016
Potential conflict of interest: Nothing to report.
- Issue online: 7 JAN 2013
- Version of Record online: 4 DEC 2012
- Accepted manuscript online: 22 AUG 2012 03:21AM EST
- Manuscript Accepted: 1 AUG 2012
- Manuscript Received: 27 MAR 2012
Additional Supporting Information may be found in the online version of this article.
|HEP_26016_sm_SuppFig1.tif||1866K||Supplementary Figure 1. Monocyte subset isolation. Monocyte subsets were isolated using the CD16+ monocyte isolation kit and CD14 microbeads from Miltenyi Biotec. Figure shows representative flow cytometry quadrants of monocytes based on CD14 and CD16 expression, after each step of the monocyte subset isolation procedure. (A) Total PBMCs were collected after Lympholyte purification and were stained for monocyte markers. (B) NK cells and granulocytes were depleted by magnetic separation and (C) CD16+ beads were used for positive selection of CD16+ monocytes. (C.a) The non-magnetically labelled cells were >90% CD14+ and (C.b) CD16+ monocytes were >94% positive. (D) An additional step to select CD14+ monocytes was included which resulted in (D.a) >95% CD14++CD16- classical monocytes and (D.b) a total CD14+CD16+ population (>92% positive) that included both intermediate CD14++CD16+ and non-classical CD14+CD16++ monocytes.|
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