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HEP_26022_sm_SuppFig1.tif18285KFigure 1S. Liver pathology after two-lobe liver IR. A. Representative sample of at least 10 mice per each group (wild type, IRF3-/- and TRIF-/- mice), showing the ischemic lobes immediately after harvest. B. Liver lobe and HE stained liver section (1 ×) and C. liver section (40 ×, and 100 × original magnification) from representative sample of 10 Sham-treated mice. D. Score of necrosis, vacuolization and congestion from Sham and IR WT, IRF3-/- and TRIF-/- mice at 48h. * p < 0.05
HEP_26022_sm_SuppFig2.tif9277KFigure 2S. Serum aminotransferase measurement, liver pathology and scoring after one-lobe liver IR. A. Serum ALT levels in wild type and IRF3-/- mice after partial ischemia (one-lobe 90-min) and 4, 20 or 48 hours reperfusion. Sham controls displayed blood sample at 4 hours after reperfusion. Results are mean values ± SEM (n = 7-13). B. Scoring of the ischemic liver sections according to the Suzuki's criteria, showing the score of necrosis. C. Representative sample of anti-Ly-6G immunostaining on 20h or 48h post-IR and Sham liver section from wild type and IRF3-/- mice (40 × original magnification). (* p < 0.05, ** p < 0.01, *** p < 0.001).
HEP_26022_sm_SuppFig4.tif7335KFigure 4S. A. Control liver histology after IL-17A neutralization. IL-17 and Ly-6G rat IgG controls plus biotin-conjugated anti-rat IgG immunostaining on control Ig and anti-IL17A mAb treated WT or IRF3-/- mice 48h post liver IR. Sections are representative of 8 independent mice per experimental group (40 x original magnification). B. MPO activity in the liver tissue 48h after I/R in 6 Sham mice, 9 control IgG-treated IR mice and 4 anti-IL-17 or anti-Ly-6G/C-treated IR mice. * p < 0.05, ** p < 0.01,
HEP_26022_sm_SuppFig5.tif9888KFigure 5S. A. Hepatic IL-23p40 mRNA contents after TLR4 engagement. Low density fraction, F4/80+ purified or CD11c+ purified cells were collected from the liver or the spleen of C57BL/6 wild type or IRF3-/- mice that were i.v. injected with vehicle (sham) or LPS 2 hours before. mRNA were extracted from a pool of 4 mice per experimental group and analyzed by real-time RT-PCR. IL-12p40 mRNA contents were normalized using ?-actin mRNA as reference. Results are mean values ± SEM and were collected from 4 independent experiments (* p < 0.05). B. Hepatic tissue IL-10, IL-1ra, TNF-a, IL-27p28 mRNA contents after TLR4 engagement. Liver fraction were collected from WT and IRF3-/- mice 1h30 and 3h after LPS treatment. Data are expressed as 5 independent mRNA relative units compared with the value obtained in the respective untreated mice (n=5). * p < 0.05.NS, non significant.
HEP_26022_sm_SuppFig6.tif154KFigure 6S. IL-27p28 and IL-23 producing M1 type liver macrophages. Liver low density fraction were stained for F4/80, CD11c, TNF-?, IL-10, IL-27p28 and IL-23p19 after in vitro LPS treatment. Data are mean percentages ± SEM from 20 WT and IRF3-/- mice collected in 3 independent experiments for F4/80+ CD11c- cells (A) and TFN-?high IL-10low producing cells (B). C. Representative percentage of IL-27p28 and IL-23p19 producing M1 type macrophage out of 3 experiments.
HEP_26022_sm_SuppFig7.tif2871KFigure 7S. IL-17A producing cells in the liver and the spleen after TLR4 engagement. A. Mononuclear cells were isolated from the liver or the spleen of a pool of 5 C57BL/6 wild type or IRF3-/- mice stimulated as described. IL-17A and IFN-? secreting cells were measured by FACS. Numbers indicated the percentage of positively stained among TCR?? T cells or CD3+ NK1.1+ gated cells. The results are representative of 3 independent experiments. B. Fold induction of liver IL-17 producing cells in LPS or vehicule treated WT or IRF3-/- mice. Mean values of induction compared to vehicle treated mice in each strain; p value are calculated with a t-test. * p < 0.05.

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