Wilson's disease: Changes in methionine metabolism and inflammation affect global DNA methylation in early liver disease


  • Potential conflict of interest: Nothing to report.

  • Supported by grant number K08DK084111 from the National Institute of Diabetes and Digestive and Kidney Diseases, by Department and Divisional funds, by University of California Davis Center for Health and Nutrition Research (CHNR), by the National Center for Research Resources (NIH) through grant #UL1 RR024146 (to V.M.), by grant number R03AA020577-01 from the National Institute on Alcohol Abuse and Alcoholism (to C.H.H.), by 2R01HD041462 from the National Institute of Child Health and Human Development (to J.M.L.), T32ES002321 from the National Institute of Environmental Health Sciences (to R.W.), and by a Veterans Health Administration Biomedical Laboratory Research and Development National Merit Review grant (to K.K.K.). V.M. is a full member of the University of California San Francisco Liver Center (Liver Center grant number P30 DK026743). The content is the sole responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health.


Hepatic methionine metabolism may play an essential role in regulating methylation status and liver injury in Wilson's disease (WD) through the inhibition of S-adenosylhomocysteine hydrolase (SAHH) by copper (Cu) and the consequent accumulation of S-adenosylhomocysteine (SAH). We studied the transcript levels of selected genes related to liver injury, levels of SAHH, SAH, DNA methyltransferases genes (Dnmt1, Dnmt3a, Dnmt3b), and global DNA methylation in the tx-j mouse (tx-j), an animal model of WD. Findings were compared to those in control C3H mice, and in response to Cu chelation by penicillamine (PCA) and dietary supplementation of the methyl donor betaine to modulate inflammatory and methylation status. Transcript levels of selected genes related to endoplasmic reticulum stress, lipid synthesis, and fatty acid oxidation were down-regulated at baseline in tx-j mice, further down-regulated in response to PCA, and showed little to no response to betaine. Hepatic Sahh transcript and protein levels were reduced in tx-j mice with consequent increase of SAH levels. Hepatic Cu accumulation was associated with inflammation, as indicated by histopathology and elevated serum alanine aminotransferase (ALT) and liver tumor necrosis factor alpha (Tnf-α) levels. Dnmt3b was down-regulated in tx-j mice together with global DNA hypomethylation. PCA treatment of tx-j mice reduced Tnf-α and ALT levels, betaine treatment increased S-adenosylmethionine and up-regulated Dnmt3b levels, and both treatments restored global DNA methylation levels. Conclusion: Reduced hepatic Sahh expression was associated with increased liver SAH levels in the tx-j model of WD, with consequent global DNA hypomethylation. Increased global DNA methylation was achieved by reducing inflammation by Cu chelation or by providing methyl groups. We propose that increased SAH levels and inflammation affect widespread epigenetic regulation of gene expression in WD. (HEPATOLOGY 2013)

The earliest phases of hepatic involvement in Wilson's disease (WD) include portal inflammation that may present as lymphocyte and neutrophil infiltrations,1 and microvesicular and macrovesicular steatosis,2 which is exhibited both clinically2 and in animal models of WD.3, 4 Previous studies on the pathogenesis of WD explored the possibilities of genetic polymorphisms in the ATP7B copper (Cu) transporter,5 alternative ATP7B gene splice variants,6 alterations in the RNA processing machinery,7 and the presence of gene modifiers.8 The mechanisms connecting Cu accumulation to hepatocyte damage are poorly understood and may include oxidative damage,9 apoptosis,10 and mitochondrial membrane cross-linking.11

Abnormal methionine metabolism occurs in animal models of hepatic Cu overload,12, 13 is connected to epigenetic regulation of gene expression,14 and could represent a link between Cu accumulation and the variety of hepatic manifestations in WD. Methionine metabolism is central to the regulation of S-adenosylhomocysteine (SAH), which inhibits methylation reactions, and is known to sensitize hepatocytes to the presence of tumor necrosis factor alpha (TNF-α).15 Similarly, by promoting increased cell division and DNA repair, inflammation could increase the demand for methylation.16, 17

SAH is the substrate for the bidirectional enzyme SAH hydrolase (SAHH) (Supporting Material Fig.). Cu may regulate methionine metabolism through its known inhibitory effect on SAHH with consequent increase in SAH, the principal inhibitor of transmethylation reactions.12, 13, 18 Cu binds noncompetitively to the SAHH enzyme and reduces its activity by releasing NAD+ cofactors.19 The regulatory role of increased Cu in down-regulation of SAHH activity, with consequent elevation of its substrate SAH and its potential secondary epigenetic effects on gene expression, suggest that methionine metabolism could be the missing link between Cu accumulation and hepatocyte damage in WD. Of note, there has been a growing interest in SAHH due to its relationship with SAH levels and gene expression in hepatic steatosis20 and human SAHH deficiency.21

We hypothesized that by regulating Sahh expression, Cu and its associated hepatic inflammation initiate alterations in methionine metabolism that affect DNA methylation status and potentially the expression of selected genes central to endoplasmic reticulum (ER) stress and lipid metabolism in WD. To test this hypothesis, we modulated Cu levels and inflammation by administering the Cu chelator penicillamine (PCA) and hepatic methylation status by administering the methyl donor betaine in the tx-j mouse model of WD.


CPT1A, carnitine palmitoyltransferase 1A; Cu, copper; DNMT, DNA methyltransferase; ER, endoplasmic reticulum; GRP78, glucose-regulated protein 78; PCA, penicillamine; PPARα, peroxisome proliferator-activated receptor alpha; SAH, S-adenosylhomocysteine; SAHH, S-adenosylhomocysteine hydrolase; SAM, S-adenosylmethionine; SREBP1c, sterol regulatory element-binding protein 1c; TNF-α, tumor necrosis factor alpha; WD, Wilson's disease.

Materials and Methods

Animal Models and Experimental Protocols

We used the C3HeB/FeJ-Atp7btx-J/J mouse (tx-j) model of WD with its background strain C3HeB/FeJ (C3H) as a control. The tx-j mouse model has a G712D missense mutation predicted to be in the second transmembrane region of the Atp7B gene, which results in a phenotypic disorder similar to WD.22 Mice in the baseline and PCA experiments were obtained from the Jackson Laboratory (Bar Harbor, ME), whereas mice in the betaine experiments were obtained from our in-house UC Davis colony that was developed from C3H breeder pairs and homozygous-affected tx-j breeder pairs purchased from the Jackson Laboratory. At 24 weeks of age, seven males from each strain were taken for harvest of blood and tissues and served as control groups for mice in PCA and betaine studies.

PCA Study.

From age 12 to 24 weeks, a subgroup of seven male tx-j mice received treatment with oral PCA (Sigma Aldrich, St. Louis, MO) that was dissolved in deionized water at 100 mg/kg bodyweight/day, a dose shown to reduce hepatic Cu concentration in a rat model of WD.23 PCA was not administered to control mice since Cu deficiency could independently modify lipid24 and methionine metabolism.25

Betaine Study.

From age 20 to 24 weeks, six male C3H and seven tx-j mice were treated with betaine (Sigma Aldrich) supplemented at 1.5% in the drinking water.26

All other methods are described in the Supporting Material.


Baseline Characteristics and Responses to PCA and Betaine Treatments

Twenty-four-week-old tx-j mice had lower body weights and higher liver/bodyweight ratios than control C3H mice (Table 1). Mean hepatic Cu concentration was more than 30 times increased in the tx-j mice and was associated with marked lobular and portal inflammation, with ∼6-fold increase in serum alanine aminotransferase (ALT) levels and increased liver Tnf-α transcript levels. There were no differences in hepatic iron levels between the groups. Liver histopathology of tx-j mice at 24 weeks of age showed lymphocytic lobular and portal infiltrates and perisinusoidal fibrosis (Fig. 1). The oral provision of PCA to tx-j mice from age 12 to 24 weeks resulted in 50% reduction of hepatic Cu and serum ALT levels, 90% reduction in liver Tnf-α expression, and concomitant improvements of both lobular and portal infiltration, whereas betaine treatment had no effect on Tnf-α transcripts or serum ALT in tx-j mice, but significantly lowered mean hepatic Cu levels in control mice by 61% and nonsignificantly lowered mean hepatic Cu levels by 30% while reducing lobular inflammation (Table 1). Hepatocyte and nuclear diameters and their ratios and hepatocyte nuclear areas were increased in tx-j mice, but were unchanged by either PCA or betaine.

Table 1. Effects of Copper Accumulation, Penicillamine and Betaine Administration
 Control (C3H) (7)Tx-j (7)Tx-j + PCA (7)Control (C3H) + betaine (6)Tx-j + betaine (7)
  1. Values are expressed as mean ± SD. In parenthesis, the number of mice in each group.

  2. Values with same letter symbol are significantly different (P < 0.05) from each other. Hepatocyte nuclear and cell diameter were calculated on 4-6 mice/group. The relative expression of Tnf-α (and other target genes in subsequent figures) was calculated using the equation 2−ΔΔCq where ΔΔCq = ΔCq(sample) − ΔCq(calibrator). Gene transcription is expressed as an n-fold difference relative to the calibrator (mean of control groups). The chosen reference gene is HPRT1 (coefficient of variation= 3.3%). See Supporting Material for additional methods. PCA: penicillamine.

Body weight (g)39.5±2.4a29.3±1.2a27.8±1.840.5±1.41d29.3±1.1d
Liver/body weight0.046±0.002a0.058±0.001a,b0.054±0.003b0.049±0.002d0.06±0.001d
Copper (liver) (μg/g d.w.)7.2±1.5a,c259±104a,b125±26b2.8±0.4c,d181±13d
Iron (liver) (μg/g d.w.)94.6±10.8100±1198.8±8.679.5±4.6101±13
ALT (plasma)(U/L)36.2±14.5a238±49a,b104±39.5b34.8±9.1d234±111d
Bilirubin (mg/dL)0.38±0.210.4±0.11b0.3±0.170.1±00.12±0.04b
Tnf-α liver transcripts (fold changes)-0.56±1.4a2.91±1.26 a,b0.28±1.8 b-1.34±1.4d2.85±1.1 d
Lobular inflammation (0-3)0±0a1.08±0.5a,b0.4±0.41a,b0±0d0.37±0.25b,d
Portal inflammation (0-3)0.12±0.2a1.66±0.51a,b1.1±0.22b0±0d1.75±0.5d
Hepatocyte nuclear diameter (N) (μm)5.6±0.2a16±2a14.7±0.195.6±0.3d15±0.8d
Hepatocyte cell diameter (C) (μm)16.9±0.9a41±4.8a40±1.817.4±1d38±2.9d
N/C ratio0.3±0.01a0.4±0.01a0.4±0.010.3±0.007d0.4±0.01d
Hepatocyte nuclear area (μm2)46±2.9a99±7.7a114±1447±0.62d101±19d
Figure 1.

Liver histology from control and tx-j mice at 24 weeks of age (A,B, hematoxylin and eosin, 10×; A, central vein; B, portal space; C, trichrome, 20×). Control (C3H) mice histology was normal (panels 1 and 4). Untreated tx-j mice presented enlarged and glycogenated hepatocyte nuclei (arrows) (2A), with marked lymphocytic lobular (2A) and portal infiltrate (arrow) (2B) and perisinusoidal fibrosis (2C). Minimal steatosis was present and was unaffected by either treatment. Lobular inflammatory infiltrate partially improved after penicillamine (arrow) (3A) and betaine treatment (arrow) (5A), with no change in portal inflammatory infiltrate after betaine treatment (arrow) (5B).

Selected Gene Expressions for ER Stress and Steatosis.

The transcript levels of selected genes related to ER stress (glucose-regulated protein 78 [Grp78]), lipogenesis (sterol regulatory element-binding protein [Srebp1c]), and fatty acid β oxidation (peroxisome proliferator-activated receptor α [Pparα] and carnitine palmitoyl transferase 1A [Cpt1A]), and protein levels of SREBP1c and PPARα were each lower in untreated tx-j than in C3H mice (Fig. 2). PCA further down-regulated Srebp1c and Pparα transcript levels and protein levels of GRP78 and CPT1A in tx-j mice. Betaine down-regulated the transcript levels of Grp78, Pparα, and Cpt1A in the control mice and Cpt1A in tx-j mice, whereas both transcript and protein levels of SREBP1c, PPARα, and CPT1A were each lower in betaine-treated tx-j mice than in betaine-treated control mice.

Figure 2.

Western blot analysis and transcripts levels of genes representative of ER stress, lipogenesis, and fatty acid oxidation. “Control” refers to untreated C3H mice in all panels. (A-D) GRP78; SREBP1c; PPARa; CPT1A, each showing protein and transcript expression data normalized to β-actin (6-7 mice/group); (E) Numerical mean ± SD of protein expressions according to Western blots, in parenthesis the number of mice used for the quantification (seven in all groups, except CPT1A (†) in six tx-j mice) in this and subsequent figures. *P < 0.05; **P < 0.001. See Supporting Information for methods. Values with the same letter symbol are significantly different (P < 0.05) from each other.

Methionine Metabolism, DNA Methyltransferases, and Global DNA Methylation in Untreated tx-j Mice

Liver S-adenosylmethionine (SAM) levels were similar in the untreated groups, whereas SAH levels were increased and SAM to SAH ratios were lower in the tx-j mice versus control mice (Fig. 3A-C). Although SAHH activity was similar in both untreated groups (Fig. 3D), both SAHH gene and protein expressions were decreased in the tx-j mice (Fig. 3F). DNA methyltransferase 1 (Dnmt1) transcripts were up-regulated, Dnmt3a transcripts were similar, and Dnmt3b transcripts were down-regulated in tx-j mice (Fig. 4A-C). According to dot blot analysis, global DNA methylation was lower in tx-j than in C3H mice (Fig. 5).

Figure 3.

(A) Hepatic S-adenosylmethionine (SAM); (B) S-adenosylhomocysteine (SAH); (C) SAM/SAH ratio; (D,E) S-adenosylhomocysteine hydrolase (SAHH) activity; (F) Western blot analyses and mRNA transcript levels. The curves for SAHH activities in C3H and tx-j mice represent the average of absorbance measurements of 6-7 mice from each group of 24-week-old mice, untreated and in response to penicillamine (PCA) (D) and betaine (E). Protein and gene transcript levels of SAHH were significantly lower in tx-j mice compared to control CH3 mice (F). Values with the same letter symbol are significantly different (P < 0.05) from each other. *P < 0.05; **P < 0.001.

Figure 4.

DNA methyltransferases transcript levels. Dnmt1 levels were up-regulated (A), whereas Dnmt3b (C) levels were down-regulated in untreated tx-j mice and further down-regulated by PCA treatment in both Dnmt3a and Dnmt3b. Betaine treatment had opposite effects on Dnmt1 and Dnmt3b expression in control and tx-j mice, while increasing the expression of Dnmt3b in the treated tx-j mice. *P < 0.05; **P < 0.001.

Figure 5.

Global DNA methylation. (A) Global DNA methylation was lower in the tx-j mice and improved to control levels after Cu chelation by PCA. Betaine treatment resulted in increased methylation in both untreated groups. (B) The red blots represent 5-methylcytosine (5-MeC) and the green blots represent total DNA signal. Each timepoint and genotype is represented by two mice (upper and lower row in each black square), each of them in triplicate. Each blot represents methylated DNA from one mouse. *P < 0.05; **P < 0.001.

Effects of PCA on SAHH Activity, Tnf-α and Dnmts Transcripts, and Global DNA Methylation

PCA treatment reduced Tnf-α transcripts by 10-fold (Table 1) and increased SAHH activity in tx-j mice (Fig. 3D), whereas Sahh gene expression remained low and protein levels were further decreased (Fig. 3F), although SAM and SAH levels and SAM/SAH ratios were unchanged (Fig. 3A-C). PCA treatment restored global DNA methylation to control levels (Fig. 5), despite unchanged Dnmt1 and reduction of Dnmt3a and Dnmt3b transcripts (Fig. 4B,C).

Effects of Betaine Treatment on SAM/SAH Ratio, Transcript Levels of Dnmt3b, and Global DNA Methylation

Betaine treatment from 20 to 24 weeks increased hepatic SAM levels in both groups, SAH levels in control mice, and the SAM/SAH ratio in tx-j mice (Fig. 3A-C). Although betaine treatment did not affect SAHH activity (Fig. 3E), it down-regulated Sahh transcripts in tx-j mice (Fig. 3F). Dnmt1 and Dnmt3a transcripts were unchanged by betaine in both groups (Fig. 4A,B), whereas Dnmt3b transcript levels were up-regulated in tx-j mice (Fig. 4C). Global DNA methylation was increased by betaine treatment in both groups (Fig. 5).

Correlations of Dnmts Transcript Levels with Global DNA Methylation and Selected Gene Expressions.

Using data from all groups, Dnmt3b expression correlated positively with global DNA methylation and with transcript levels of Sahh, Grp78, Srebp1c, Pparα, and Cpt1A (Table 2). In addition (not shown), global DNA methylation values correlated with Srebp1c and Pparα transcript levels (r = 0.39, P = 0.02 and r = 0.41, P = 0.02, respectively).

Table 2. Correlation Analysis of the Relationship Between Dnmts Transcript Levels with Global DNA Methylation and the Expressions of the Studied Genes
 Global DNA methylationSahhGrp78Srebp1cPparαCpt1A
 rP valuerP valuerP valuerP valuerP valuerP value
  1. Sahh, S-adenosylhomocysteine hydrolase; Grp78, glucose-related protein 78; Srebp1c, sterol regulatory element-binding protein 1c; Pparα, peroxisome proliferator-activated receptor α; Cpt1A, carnitine palmitoyltransferase 1A; Dnmt, DNA methyltransferase.


Immunostaining for 5-Methylcytosine.

The immunostaining pattern for 5-methylcytosine showed diffuse and less intense signals in hepatocyte nuclei from tx-j mice than in C3H mice (Fig. 61A, 2A). Normalized signal intensity was significantly higher in C3H mice than in tx-j mice, but not significantly different in betaine treated tx-j and control mice. In addition, when comparing betaine versus PCA treated tx-j mice, betaine treatment was associated with stronger nuclear intensity peaks (Fig. 62B,2C), indicating that provision of methyl groups is associated with a different pattern of DNA methylation.

Figure 6.

Immunostaining for 5-methylcytosine. Compared to C3H mice (1A), untreated tx-j mice presented diffuse and less intense nuclear staining (2A) (P < 0.01). Betaine-treated control (1B) and tx-j mice (2C) had similar immunostaining patterns. Betaine-treated tx-j (2C) presented a higher percentage of nuclear peaks than PCA-treated tx-j mice (2B) (P = 0.01). Arrowheads in the insets indicate representative nuclei.


This study investigated the potential role of Cu-induced abnormal methionine metabolism in the tx-j mouse model of WD and found several novel results relevant to treatment. First, elevated levels of SAH and reduced levels of the SAM to SAH methylation ratio were observed in untreated tx-j mice in association with reduced levels of SAHH and global DNA methylation. DNA hypomethylation in tx-j mice was correlated with reduced expression of Dnmt3b, but was paradoxically associated with increased expression of Dnmt1. Second, Cu chelation by PCA improved inflammation in the tx-j mice, reduced the expression of Tnf-α and selected genes related to ER stress and lipid metabolism, and normalized global DNA methylation levels while reducing transcript levels of Dnmt3a and Dnmt3b. Lastly, the methyl donor betaine also normalized global DNA methylation while enhancing SAM levels and SAM-to-SAH ratio and reducing transcript levels of Cpt1A in the tx-j mice.

We propose that interplay between inflammation and methionine metabolism is related to Cu-mediated inhibition of Sahh resulting in elevated SAH levels, which dysregulates methylation status and gene expression in WD. We demonstrated that Sahh transcript and protein levels were down-regulated in the tx-j mouse model of WD, whereas SAHH activity was increased by Cu chelation with PCA, although liver SAH levels remained increased and the SAM/SAH level decreased. A previous study on the natural course of WD in 24-week-old LEC rats showed reduced SAHH protein levels only in advanced stages of liver disease,27 in contrast to findings in the present study of the same age tx-j mice.

SAH is a potent inhibitor of DNMTs activities and reduced levels of Dnmts transcripts were described in response to elevated SAH levels in a recent clinical study.28 Notably, we observed that Dnmt1, which encodes the principal DNMT in mammalian cells and is mainly implicated in the maintenance of methylation status, was up-regulated, whereas Dnmt3b, encoding a de novo methyltransferase, was down-regulated in untreated tx-j mice, and both Dnmt3a and Dnmt3b transcripts were down-regulated by PCA. Transcript levels of Dnmt genes show compensatory increased levels in response to reduced DNA methylation in liver or brain.29 Others described up-regulation of Dnmt1 expression without changes in Dnmt3a in livers of choline-deficient rat embryos with global DNA hypomethylation,30 whereas Dnmts levels are increased in chronic hepatitis, cirrhosis, and human hepatocellular carcinoma.31 The finding that Dnmt3b was down-regulated in untreated tx-j mice with increased SAH levels and correlated with global DNA methylation and expressions of each of the studied genes suggests that this enzyme plays a major role in controlling global DNA methylation in WD. There were some discrepancies in the Dnmts expression in response to betaine treatment. We did not observe any change in Dnmts levels in control mice nor in Dnmt1 and Dnmt3a in tx-j mice, whereas Dnmt3b was markedly up-regulated in tx-j mice in response to betaine.

Tx-j mice demonstrated reduced levels of global DNA methylation, which was restored either by PCA-induced Cu chelation or by provision of the methyl donor betaine. The positive effect of PCA on increasing global DNA methylation may be due to relatively reduced inflammation and demand for methyl groups.17 At the same time, methyl group supplementation by betaine could increase global DNA methylation in both control and tx-j mice, regardless of inflammation. We propose that global DNA hypomethylation in WD was influenced by inflammation which was resolved by reducing Cu hepatic levels by PCA treatment, or by the increasing availability of methyl groups by betaine. The major difference between PCA and betaine treatment in tx-j mice was that PCA was exclusively associated with a significant improvement of inflammation, whereas only betaine induced significant increased SAM and Dnmt3b levels. These observations suggest a potential positive additive effect of including betaine in the PCA treatment of patients with WD.

Methyl donors such as betaine and/or SAM have been used effectively in treatment of other liver diseases, including adjuvant therapy for hepatitis C in which either agent restored methylation of STAT1 which regulates the response to interferon treatment,32 whereas SAM improved viral kinetics by increasing the methylation of STAT1 and the expressions of its representative genes.33 A study of experimental acetaminophen liver toxicity found that SAM administration restored its mitochondrial and nuclear levels, possibly due to increased expression and activity of methionine adenosyltransferase.34 These results support our findings that the administration of the methyl donor betaine modified SAM levels and consequently the expressions of selected genes.

The observed significant 61% reduction of the mean liver Cu level by betaine treatment in control mice and nonsignificant 30% reduction of mean hepatic Cu level in the tx-j mice (Table 1) are original findings, which could have been influenced by uneven hepatic Cu distribution in the liver.35 Potentially, betaine could reduce Cu levels by modification of the expressions of genes for Cu chaperones or cellular transport. Ultimately, only long-term studies on betaine treatment for tx-j mice may clarify this issue.

Currently, the molecular mechanisms linking Cu and gene expression are unknown but it seems likely that methylation plays an important role. Reduced global methylation in the untreated tx-j mice could relate to increased inflammation, which others have associated with increased demand for gene methylation resulting from increased cell division and DNA.16, 17 Consequently, WD may be associated with increased demand for methyl groups as result of both an increase of the methylation inhibitor SAH and increased inflammation. Although the associations of Cu-mediated SAHH inhibition with SAH accumulation and reduced Dnmt3b transcripts were consistent with global DNA hypomethylation in tx-j mouse livers, the relationship between DNA hypomethylation and down-regulation of the expressions of genes representative of ER stress, lipogenesis, and fatty acid oxidation in untreated tx-j mice is less clear. Similar to our data, others described down-regulation of gene transcripts representative of lipid metabolism in the Atp7b−/− knockout mouse at 6 weeks of age, prior to development of histological damage.36 However, this study did not find any changes in Sahh and Dnmt1 expressions, whereas Dnmt3a, Dnmt3b, and potential changes in lipid metabolism in response to Cu chelation were not measured. In the present study, the hypothesis that the observed changes in gene transcript levels with PCA treatment are due to changes in methylation is supported by observations that both PCA and betaine treatments were associated with significant increases in global DNA methylation and by the positive correlations between Dnmt3b transcripts and global DNA methylation and transcripts of genes representative of ER stress, lipogenesis, and fatty acid oxidation.

There are potentially other mechanisms involved in the gene expression response to Cu accumulation and PCA. As discussed by others, various proteins could mediate crosstalk between Cu status and lipid metabolism,37 and others showed that Cu enters nuclei where it can bind to specific targets.38

The observed changes in hepatocellular morphology with increased hepatocyte and nuclear diameter, and nuclear to hepatocyte diameter ratios that persisted with treatment, are novel, and differ from previously reported data in the LEC rat where they responded to Cu chelation therapy.23 Nuclear and cellular hepatocyte enlargement, which in general are associated with chronic hepatitis with hepatocyte enzymatic activation,39 is well known in humans with WD1, 40 and in animal models of this condition3, 23 but the response to Cu chelation treatment has not been consistently described.41

Prior studies on the effects of betaine included its down-regulation of selected ethanol-induced ER stress-related genes Grp78 and Srebp1c in mice,42 that Ppara promoter methylation in offspring was affected by maternal dietary betaine in rats,43 and that the Cpt1A promoter is hypermethylated in human embryonic stem cells.44 Although both PCA and betaine treatments were associated with increased global DNA methylation in our study, betaine treatment achieved significant down-regulation of selected ER stress and fatty acid oxidation related transcripts only in control mice, i.e., specifically in the absence of inflammation and Cu accumulation. Increased methylation of promoter CpG islands has been correlated with gene silencing, and may be occurring in our control mice with normally saturated levels of DNA methylation following betaine treatment. In contrast, gene body and intergenic methylation were unsaturated in the global hypomethylated state that we observed in the tx-j mouse livers, so increasing the availability of methyl groups can be predicted to target nonpromoter methylation sites associated with increased gene expression levels.45 Of note, the directions and magnitude of changes in proteins levels for the studied genes were generally similar to those in transcript levels. It is possible that the observed differences between messenger RNA (mRNA) and protein levels of several of the studied genes could be attributed to posttranslational modifications.

In summary, WD appears to be a condition associated with increased demand for methyl groups due to both increased levels of the methylation inhibitor SAH, which is based on the inhibitory effect of Cu on SAHH as shown by its activation after PCA chelation, and also due to chronic inflammatory status (Supporting Fig.). Our results demonstrate reduced SAHH levels in a mouse model of WD that is characterized by Cu accumulation and hepatic inflammation in the early phases of liver damage. The consequent elevation of liver SAH as a result of SAHH inhibition, and the concomitant inflammation, were associated with down-regulation of Dnmt3b and global DNA hypomethylation. Our study supports the hypothesis that gene expression and possibly phenotypic presentations of WD are affected by interactions between Cu accumulation and methionine metabolism.


We thank Dr. Robert D. Cardiff for expert assistance with the histopathology analysis.