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Additional Supporting Information may be found in the online version of this article.

FilenameFormatSizeDescription
HEP_26081_sm_SuppFig1.tif218KSupplementary Figure 1. A CDAA diet intake was measured on WT and TLR2-/- mice.
HEP_26081_sm_SuppFig2.tif571KSupplementary Figure 2. WT-Kupffer cells and HSCs were treated with palmitic acid, ranging from 0 to 500 μM. (A) mRNA expression of TNFα and CD68 in WT Kupffer cells. Genes were normalized to 18S RNA as an internal control. (B) TNFα concentrations in supernatant from culture of WT Kupffer cells. N.D; not detected. (C) mRNA expression of fibrogenic genes in WT HSCs.
HEP_26081_sm_SuppFig3.tif574KSupplementary Figure 3. HSCs were isolated from WT mice and cultured in the presence of 5 μg/ml Pam3CK4 and/or 200 μM palmitic acid. (A) mRNA expression of IL-1β (B) active IL-1β in the supernatant. (C) mRNA expression in NLRP3. Genes were normalized to 18S RNA as an internal control. (D) Caspase-1 activity. N.D; not detected. N.S; not significant. Data represent mean ± SD.
HEP_26081_sm_SuppFig4.tif864KSupplementary Figure 4. WT hepatocytes were isolated in combination with clodronate liposome treatment to deplete comtaminted Kupffer cells from hepatocytes. These hepatocytes were cultured in the presence of 5 βg/ml Pam3CK4 and/or 200 βM palmitic acid. (A) mRNA expression of IL-1β. (B) Active IL-1β in the supernatant. (C) mRNA expression in NLRP3. (D) Caspase-1 activity. (E) mRNA expression of TNFα. (F) TNFα in the supernatant. Genes were normalized to 18S RNA as an internal control. N.D; not detected. Data represent mean ±SD, *p<0.05. N.S.; not significant.
HEP_26081_sm_SuppFig5.tif249KSupplementary Figure 5. WT mice were fed standard chow, CSAA diet, and CDAA diet for 22 weeks, and MCD diet for 8 weeks. Blood was harvested from the portal vein, and FFA concentrations were examined. Data represent mean ±SD, *p<0.05.

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