Differentiation capacity of hepatic stem/progenitor cells isolated from D-galactosamine-treated rat livers†
Article first published online: 27 JAN 2013
Copyright © 2012 American Association for the Study of Liver Diseases
Volume 57, Issue 3, pages 1192–1202, March 2013
How to Cite
Ichinohe, N., Tanimizu, N., Ooe, H., Nakamura, Y., Mizuguchi, T., Kon, J., Hirata, K. and Mitaka, T. (2013), Differentiation capacity of hepatic stem/progenitor cells isolated from D-galactosamine-treated rat livers. Hepatology, 57: 1192–1202. doi: 10.1002/hep.26084
Potential conflict of interest: Nothing to report.
- Issue published online: 28 FEB 2013
- Article first published online: 27 JAN 2013
- Accepted manuscript online: 18 SEP 2012 06:12AM EST
- Manuscript Accepted: 8 SEP 2012
- Manuscript Received: 31 JAN 2012
- Ministry of Education, Culture, Sports, Science, and Technology, Japan, Grant-in-Aid for Scientific Research (C). Grant Number: 19566021
- Grants-in-Aid for Young Scientists (B). Grant Numbers: 22790385, 19790294
- Yuasa Memorial Foundation
- Grants-in-Aid for Scientific Research (B). Grant Numbers: 22390259, 21390365
Additional Supporting Information may be found in the online version of this article.
|HEP_26084_sm_SuppFig1.tif||478K||Supporting Information Figure 1. FISH was performed using a rat Y chromosome probe in a liver section of a positive control (male) (A), negative control (female) (B), and the cells derived from transplanted CD44+ cells (C, D). White arrowheads show Y chromosome-positive cells.|
|HEP_26084_sm_SuppFig2.tif||771K||Supporting Information Figure 2. Phase-contrast photos of every colony were taken and qPCR was performed for each colony. The gene expression patterns of the colonies were divided into roughly 2 groups by the intensity of CD44 expression. Compared to the cells in CD44low colonies (#1-4), those in CD44high colonies (#5-8) showed not only relatively high expression of albumin and HNF4??????? but also suppression of CK19 expression.|
|HEP_26084_sm_SuppFig3.tif||52K||Supporting Information Figure 3. To induce the maturation of cells in Thy1-derived colonies, the cells were treated with Matrigel®. The gene expression patterns of Thy1, CD44, HNF4α and CK19 were analyzed (A). The content of albumin secreted to the medium was measured by ELISA (B). Asterisks show significant differences: p<0.01|
|HEP_26084_sm_SuppTables.doc||59K||Supporting Information Tables.|
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