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Additional Supporting Information may be found in the online version of this article.

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HEP_26239_sm_SuppFig1.tif2418KSupporting Information Figure 1. Mice characterization. Lymphocytes from thymus, lymph nodes (8 periphery lymph nodes), spleen and liver were isolated, (A) total cell numbers were counted, (B) CD4+ and CD8+ cells, (C) NK and NKT cells, (D) CD4+Foxp3+ Treg cells and (E) activation marker were analyzed by flow cytometry. Naïve, indicate the CD44low, CD62Lhigh population; CM, indicate the CD44high, CD62Lhigh population; EM, indicate the CD44high, CD62Llow population (n=4). p>0.05. Data represent three independent experiments with similar results.
HEP_26239_sm_SuppFig2.tif833KSupporting Information Figure 2. Time courses of serum p28 levels after ConA injection. 5 mg/kg ConA was injected into WT, EIIa-p28f/f and CD11c-p28f/f mice. Blood sera were collected at 0, 1, 2, 4, 6, 8, 12 and 24 hours post ConA injection. Serum p28 levels were measured by ELISA (n=8). Data represent at least three independent experiments with similar results.
HEP_26239_sm_SuppFig3.tif4954KSupporting Information Figure 3. Inflammation mediated liver injury. (A) 10 mg/kg ConA was injected into WT and CD11c-p28f/f mice. Blood sera were collected at 2 hours post ConA injection. Cytokine levels were measured by ELISA (n=12). (B) 1mg/kg anti-Fas (clone Jo2) were i.p. injected into WT and CD11c-p28f/f mice. 12 hours post injection, liver tissues were fixed for H&E staining, and one representative tissue staining is shown. Scale bars, 200μm. N, necrosis area. Percentages of necrosis were calculated. p>0.05. Data represent two independent experiments with similar results.
HEP_26239_sm_SuppFig4.tif1460KSupporting Information Figure 4. Cell depletion. (A) Control antibody and anti-NK1.1 mAb or (B) anti-CD4 mAb was injected into WT and CD11c-p28f/f mice. 24 hours after injection, liver lymphocytes were isolated, and analyzed by flow cytometry (n=5). Data represent three independent experiments with similar results.
HEP_26239_sm_SuppFig5.tif175KSupporting Information Figure 5. Hydrodynamic injection of p28 plasmid. (A) Control plasmid and recombinant p28 plasmid were hydrodynamic injected into WT and CD11c-p28f/f mice 72 hours prior to ConA treatment, followed by ConA injection (20 mg/kg ConA for WT mice, and 5 mg/kg for CD11c-p28f/f mice). Blood sera were collected at 12 hours post ConA injection. ALT, (B) IFN-γ and (C) p28 levels were measured (n=8). Data represent three independent experiments with similar results.
HEP_26239_sm_SuppFig6.tif2196KSupporting Information Figure 6. Adoptive transfer of CD4+ T cells. (A) CD4+ T cells were sorted from mice spleen as shown by the gate (left panel). Sorted cells were analyzed by flow cytometry (right panel). (B) PBS or sorted CD4+ T cells from WT, CD11c-p28f/f and CD11c-p28f/f-IFN-γ-/- mice were transferred into CD4-/- mice. 24 hours after transfer, lymphocytes from blood, spleen and liver were analyzed by flow cytometry (n=5). Data represent two independent experiments with similar results.
HEP_26239_sm_SuppFig7.tif1983KSupporting Information Figure 7. Analysis of DC. (A) Flow cytometry analysis of splenic CD11c+MHCII+ DC in WT and CD11c-p28f/f mice (n=4). p>0.05. (B) Surface expression of CD80 and CD86 on unstimulated WT and CD11c-p28f/f splenic DC. (C) WT and CD11c-p28f/f splenic DC were sorted and stimulated with or without 100ng/ml LPS for 5 hours. Cytokine mRNA levels were analyzed by quantitative real-time PCR (n=3). (D) BMDC from WT and CD11c-p28f/f mice were stimulated with or without 100ng/ml LPS for 5 hours. Cytokine mRNA levels were analyzed by quantitative real-time PCR (n=3). (E) BMDC from WT and CD11c-p28f/f mice were stimulated with or without 100ng/ml LPS for 30 minutes. Cell lysates were analyzed by Western blot (n=3). Data represent three independent experiments with similar results.
HEP_26239_sm_SuppInfo.doc56KSupporting Information

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