SEARCH

SEARCH BY CITATION

Additional Supporting Information may be found in the online version of this article.

FilenameFormatSizeDescription
HEP_26192_sm_SuppFig1.tif1643KSupporting Information Figure 1. Paraffin-embedded samples were stained with anti-NK-1 Ab. Distribution of NK cells in human normal liver (n = 9), chronic hepatitis liver (n = 7), remote nontumoral liver (stage I and II: n = 74; stage III and IV: n = 21) and intratumoral tissues (stage I and II: n = 178; stage III and IV: n = 78) of HCC. Bar: 150μm.
HEP_26192_sm_SuppFig2.tif609KSupporting Information Figure 2. Fresh lymphocytes were isolated from paired peripheral blood, nontumoral liver and intratumoral tissues of HCC patients. CD56+CD3− NK cells were set in the lymphocyte region determined by an FSC and SSC dot plot. A representative gating illustration was shown.
HEP_26192_sm_SuppFig3.tif684KSupporting Information Figure 3. Definition of nontumor, tumor tissue, invading edge, peritumor stroma and intratumoral regions in human HCC.
HEP_26192_sm_SuppFig4.tif71KSupporting Information Figure 4. Role of 2B4 and CD48 interaction in regulating NK cell function by autologous TSN-treated monocytes/Mϕ. (A-B) Monocytes from healthy PBMC were incubated in medium alone (MO) or with 15% of the culture supernatant from a hepatoma cell line (HepG2; TSN-MO) for 12 hours. After washing, these monocytes were cultured with autologous NK cells for indicated times (n = 5). Percentages of CD69+, Ki67+, IFN-γ+ and TNF-a+ NK cells were determined by FACS. Dotted line: Medium alone, Dash line: MO, Solid line: TSN-MO. (C) Levels of CD48 expression in monocytes (MO) and TSN-treated monocytes (TSN-MO) were determined by FACS (n = 5). (D) Monocytes (MO) and TSN-treated monocytes (TSN-MO) were cultured with autologous NK cells in the presence or absence of 4 μg/mL anti-2B4 Ab, or isotype control Ab (n = 5). IFN-γ+ and TNF-a+ NK cells after 8-day coculture were determined by FACS. Results are expressed as means ± SEM. *P < 0.05; **P < 0.01.
HEP_26192_sm_SuppFig5.tif1116KSupporting Information Figure 5. Contact between CD68+ cells (red) and NK-1+ cells (green) in peritumoral stroma was analyzed by confocal microscopy. DAPI, blue. 1 out of 10 representative micrographs was shown.
HEP_26192_sm_SuppFig6.tif674KSupporting Information Figure 6. Fresh lymphocytes were isolated from paired nontumoral liver and intratumoral tissues of HCC patients (n = 5). Levels of NKG2D and NKp30 expression in NK cells were analyzed by FACS. Results are expressed as means ± SEM.
HEP_26192_sm_SuppInfo.doc31KSupporting Information
HEP_26192_sm_SuppTables.doc59KSupporting Information Tables.

Please note: Wiley Blackwell is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.