ASC/caspase-1/IL-1β signaling triggers inflammatory responses by promoting HMGB1 induction in liver ischemia/reperfusion injury†
Version of Record online: 15 MAY 2013
Copyright © 2013 American Association for the Study of Liver Diseases
Volume 58, Issue 1, pages 351–362, July 2013
How to Cite
Kamo, N., Ke, B., Ghaffari, A. A., Shen, X.-d., Busuttil, R. W., Cheng, G. and Kupiec-Weglinski, J. W. (2013), ASC/caspase-1/IL-1β signaling triggers inflammatory responses by promoting HMGB1 induction in liver ischemia/reperfusion injury. Hepatology, 58: 351–362. doi: 10.1002/hep.26320
Potential conflict of interest: Nothing to report.
- Issue online: 24 JUN 2013
- Version of Record online: 15 MAY 2013
- Accepted manuscript online: 13 FEB 2013 09:01AM EST
- Manuscript Accepted: 7 FEB 2013
- Manuscript Received: 6 SEP 2012
- The National Institutes of Health. Grant Numbers: RO1 DK062357 to Jerzy W. Kupiec-Weglinski, AI056154 to Genhong Cheng
- The Diann Kim Foundation
- The Dumont Research Foundation
Additional Supporting Information may be found in the online version of this article.
|HEP_26320_sm_SuppFig1.tif||1717K||Supporting Information Figure 1. Representative immunohistochemical staining for HMGB1 in hepatocytes (A/B) or infiltrating macrophages/monocytes (C/D) harvested from WT vs. ASC KO livers (6h of reperfusion after 90min of warm ischemia). Magnification ×400, n=3-4/group (*p<0.0001).|
|HEP_26320_sm_SuppFig2.tif||1715K||Supporting Information Figure 2. Histopathology of ASC-deficient livers at 90min of warm ischemia only (A) vs. 90min ischemia plus 6h reperfusion (B). Representative of n=3-5/group; Magnification ×200.|
|HEP_26320_sm_SuppFig3.tif||649K||Supporting Information Figure 3. BMMs from ASC KO or WT mice were cultured with rHMGB1 (1μg/ml) for 24h. The expression of cleaved caspase-1 and TLR4 was evaluated by Western blots. rHMGB1 supplement increased the expression of cleaved caspase-1/TLR4 (A), and IL-1β (B, p<0.01) in both ASC-deficient and WT groups, compared with BMM controls without rHMGB1 adjunct. Representative of three experiments.|
|HEP_26320_sm_SuppFig4.tif||648K||Supporting Information Figure 4. BMMs and liver tissue proteins were isolated from ASC KO and WT mice. HMGB1 expression was evaluated by Western blots. The baseline HMGB1 levels were decreased both in vitro (A) and in vivo (B) in ASC KO group, as compared with WT controls. Representative of three experiments.|
|HEP_26320_sm_SuppFig5.tif||1715K||Supporting Information Figure 5. Liver histopathology (A) and Suzuki's scores (B) in WT mice infused with rHMGB1 immediately at reperfusion (0.8mg/kg, i.p. after 90min of ischemia). Sham controls underwent the same procedure without vascular occlusion. Unlike in sham controls, treatment with rHMGB1 augmented hepatocellular injury in WT mice subjected to liver IR. Magnification ×200. Representative of n=4/group. (*p<0.0001).|
|HEP_26320_sm_SuppTab1.tif||231K||Supporting Information Table 1.|
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