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Additional Supporting Information may be found in the online version of this article.

FilenameFormatSizeDescription
HEP_26345_sm_SuppFig1.tif213KSupporting Information Figure 1. Flow cytometry histogram showing the percentage of Gdeghigh cells in the HCC primary cell and HCC cell lines.
HEP_26345_sm_SuppFig2.tif378KSupporting Information Figure 2. Gdeghigh cell form slightly larger spheroid than unsorted cells in HuH7 and Hep3B.
HEP_26345_sm_SuppFig3.tif187KSupporting Information Figure 3. The chemo-sensitivity to 5-FU anticancer drug in Gdeg high HuH7 cells and unsorted cells sorted under hypoxic mimic condition (CoCl2:100 μM). vertical bars, standard deviation.
HEP_26345_sm_SuppFig4.tif198KSupporting Information Figure 4. The percentage of GdeghighROSlow cells immediately after isolation by FACS to Day4.
HEP_26345_sm_SuppFig5.tif627KSupporting Information Figure 5. Flow cytometry histogram showing expression of classic CSCs markers ,CD133, EpCAM, CD44 and CD90 positive cells in the GdeghighROSlow HCC cell lines and unsorted HCC cells.
HEP_26345_sm_SuppFig6.tif359KSupporting Information Figure 6. Patients were divided into three subtypes based on the expression profiles of 43 upregulated probe sets of GdeghighROSlow HCC. (A) Heat map shows the correlation of three subtypes with the expression CSCs markers (EpCAM, CD44, CD90 and CD133) and biliary/progenitor cell markers (cytokeratin 7 and cytokeratin 19). (B) Significant correlation between the GdeghighROSlow HCC gene signature with the expression level of the stem/progenitor markers (p<0.001).
HEP_26345_sm_SuppTab1.doc33KSupporting Information Table 1. Percentage of GdeghighROSlow Cells of HCC Cell Lines.
HEP_26345_sm_SuppTab2.doc35KSupporting Information Table 2. The Days When The Tumors Were Visible After Inoculation.
HEP_26345_sm_SuppTab3.doc37KSupporting Information Table 3. List of Significant Gene Sets by GSEA Between GdeghighROSlow and GdeglowROShigh HCC Cells.
HEP_26345_sm_SuppTab4.doc189KSupporting Information Table 4. GdeghighROSlow HCC Cells Gene Signatures.
HEP_26345_sm_SuppTab5.doc48KSupporting Information Table 5. Clinicopathologic Characteristics of HCC Subtypes Defined by Gene Expression Profiles Upregulated in GdeghighROSlow HCC Cells
HEP_26345_sm_SuppMov.AVI636KSupporting Information Movie. Asymmetric cell division of the Gdeghigh HCC cell, observed by time-lapse microscope. The Gdeghigh HCC cell divided asymmetrically into Gdeghigh and Gdeglow HCC cells.

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