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Additional Supporting Information may be found in the online version of this article.

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hep26378-sup-0001-suppinfo.doc9398K

Supplement Figure S1 Expression of mature miR-15 family members in gastrointestinal cancers

Supplement Figure S2 Establishment of stable cell lines highly expressing miR-195

Supplement Figure S3 MiR-195 inhibited Huh-7, SGC-7901 and LOVO cell proliferation in vitro.

Supplement Figure S4 MiR-195 inhibited Huh-7, SGC-7901 and LOVO cell migration and invasion in vitro.

Supplement Figure S5 MiR-195 has no obvious effects on cell growth with matrigel within 40 h after transfection

Supporting Figure S6 No difference between negative control and miR-195 mutant RNA in cell proliferation and colony formation.

Supporting Figure S7 No difference between negative control and miR-195 mutant RNA in cell migration and invasion.

Supporting Figure S8 Western blotting assays of IKKα or TAB3 expression

Supporting Table S1 Primer sequences for constructs and DNA copy number assay

Supporting Table S2 The sequences of siRNA used in this study

Supporting Table S3 Roche probes used in this study

Supporting Table S4 ABI TaqMan miRNA probes for miR-15 family

Supporting Table S5 Antibodies used in this study

Supporting Table S6 Target prediction by TargetScan

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