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Additional Supporting Information may be found in the online version of this article.

FilenameFormatSizeDescription
hep26380-sup-0001-suppfig1.tif8119KSupporting Figure 1 Adenoviral constructs with eGFP and human FTCD led to expression in hepatoma cells latter is recognized by sera of AIH patients (A) Human FTCD, murine FTCD and control eGFP were cloned in a replication-deficient Adenovirus (Ad5). The vector map of AdEasy-1-hFTCD is shown. (B) Western blots of Ad-hFTCD transduced 293 (left panel) or HepG2 (right panel) cells. The expression of human FTCD was verified with a representative anti-LC1-positive human serum (LC1) and a representative normal donor serum (ND).
hep26380-sup-0002-suppfig2.tif2224KSupporting Figure 2 Adenoviral cleaning in the liver within the first weeks after infection PCR for adenovirus were performed of hepatic DNA of Ad-eGFP (n=6), Ad-hFTCD (n=11) infected NOD mice at week 12, plasmid DNA of Ad-hFTCD (+) and water control (-).
hep26380-sup-0003-suppfig3.tif2850KSupporting Figure 3 pIRES-eGFP-mFTCD with myc-tag led to expression in hepatoma cells Western blot of 293 cells transfected with pIRES-eGFP-mFTCD (right lanes) or control pIRES-eGFP transfected 293 cells (left lanes). The 58.8 kD FTCD construct was detected by an anti-myc-tag antibody (left panel) or a representative serum from an Ad-hFTCD infected NOD mouse (right panel).
hep26380-sup-0004-suppfig4.tif4603KSupporting Figure 4 Massive hepatic infiltrations and fibrosis in Ad-hFTCD infected mice (A) Liver sections were quantitative analyzed at week 12 using the average size of intrahepatic infiltrates. (B) Representative H&E stainings of C57Bl/6 and FVB/N 12 weeks after Ad-hFTCD infection.
hep26380-sup-0005-suppfig5.tif3928KSupporting Figure 5 Additional characterization of IHLs Representative multi-lineage characterization of IHLs from Ad-hFTCD (n=9) infected mice via flow cytometry is shown. Indicated are the populations of the NK and CD3+ T cells (left panels) and the αβTCR and γδT cell populations (right panels).
hep26380-sup-0006-suppfig6.tif4925KSupporting Figure 6 Recombinant mFTCD and unaltered cytokine levels in sera of Ad-hFTCD infected NOD mice (A) Coomassie staining of recombinant mFTCD produced in BL21 and purified using Ni-NTA. (B) Cytokine analysis of sera from Ad-eGFP or Ad-hFTCD infected mice at week 12. Concentration (pg/ml) were determined by multiplex technology and mean values are shown (±SD) (n=10).

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