Potential conflict of interest: Nothing to report.
Hedgehog signaling pathway regulates autophagy in human hepatocellular carcinoma cells
Article first published online: 30 JUL 2013
© 2013 by the American Association for the Study of Liver Diseases
Volume 58, Issue 3, pages 995–1010, September 2013
How to Cite
Wang, Y., Han, C., Lu, L., Magliato, S. and Wu, T. (2013), Hedgehog signaling pathway regulates autophagy in human hepatocellular carcinoma cells. Hepatology, 58: 995–1010. doi: 10.1002/hep.26394
Supported by National Institutes of Health grants CA106280, CA102325, CA134568, and DK077776 (to T.W.).
- Issue published online: 29 AUG 2013
- Article first published online: 30 JUL 2013
- Accepted manuscript online: 16 MAR 2013 02:15AM EST
- Manuscript Accepted: 7 MAR 2013
- Manuscript Revised: 25 FEB 2013
- Manuscript Received: 20 NOV 2012
- National Institutes of Health grants. Grant Numbers: CA106280, CA102325, CA134568, DK077776
Additional Supporting Information may be found in the online version of this article.
|hep26394-sup-0001-suppfig1.tif||4885K||SUPPORTING Figure S1. Inhibition of Hh signaling induces autophagy. (A) MDC (monodansylcadaverine) staining. Huh7 cells grown in 96-wells plates were treated with vehicle, SAG (0.5 μM), Pur (10 μM), Shh (0.4 μg/ml), GANT61 (20 μM) or GDC0449 (20 μM) for 24 hours. MDC staining was performed and the cells were examined under a fluorescence microscope. (B) Transmission electron microscopy showed autophagosomes and autophagolysosomes (arrow indicated) in GANT61-treated Huh7 cells.|
|hep26394-sup-0002-suppfig2.tif||405K||SUPPORTING Figure S2. GANT61 does not increase ATG gene expression. Huh7 (A), Hep3B (B) and HepG2 (C) cells were treated for 48 hours with 0.5 μM SAG, 10 μM Pur, 0.4 μg/ml Shh, and 20 μM GANT61, respectively. The expression of ATG genes (ATG 3, 5, 6, 7, 12) was examined by Western blotting.|
|hep26394-sup-0003-suppfig3.tif||276K||SUPPORTING Figure S3. Western blotting for NF-κB (A), p53 (B) and DNA-methyltransferase 1 and 3a (C) in Huh7 cells treated with 0.5 μM SAG, 10 μM Pur or 20 μM GANT61, respectively, for 48 hours.|
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