Additional Supporting Information may be found in the online version of this article.

hep26394-sup-0001-suppfig1.tif4885KSUPPORTING Figure S1. Inhibition of Hh signaling induces autophagy. (A) MDC (monodansylcadaverine) staining. Huh7 cells grown in 96-wells plates were treated with vehicle, SAG (0.5 μM), Pur (10 μM), Shh (0.4 μg/ml), GANT61 (20 μM) or GDC0449 (20 μM) for 24 hours. MDC staining was performed and the cells were examined under a fluorescence microscope. (B) Transmission electron microscopy showed autophagosomes and autophagolysosomes (arrow indicated) in GANT61-treated Huh7 cells.
hep26394-sup-0002-suppfig2.tif405KSUPPORTING Figure S2. GANT61 does not increase ATG gene expression. Huh7 (A), Hep3B (B) and HepG2 (C) cells were treated for 48 hours with 0.5 μM SAG, 10 μM Pur, 0.4 μg/ml Shh, and 20 μM GANT61, respectively. The expression of ATG genes (ATG 3, 5, 6, 7, 12) was examined by Western blotting.
hep26394-sup-0003-suppfig3.tif276KSUPPORTING Figure S3. Western blotting for NF-κB (A), p53 (B) and DNA-methyltransferase 1 and 3a (C) in Huh7 cells treated with 0.5 μM SAG, 10 μM Pur or 20 μM GANT61, respectively, for 48 hours.
hep26394-sup-0004-suppinfo.doc52KSupporting Information

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