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FilenameFormatSizeDescription
hep26399-sup-0001-suppfig1.tif4790KSupporting Figure 1. Metabolic context in Gnmt-/- mice. 3-month-old wild type (WT) and Gnmt-/- (KO) mice were fasted 3 hours before experiments were performed. (a) Glucose and insulin tolerance tests were assayed after oral administration of glucose (2mg/kg) or intraperitoneal injection of insulin (1U/kg). (b) Serum fatty acid (FA) concentrations. (c) Food intake. (d) Body weight. (e) Percentage of liver weight (f). Percentage of white adipose tissue (WAT). Values are mean±SEM of 4 animals per group. Statistical differences between Gnmt-/- and WT mice are denoted by **p<0.01; ***p<0.001 (Student's t test) and by two-way ANOVA.
hep26399-sup-0002-suppfig2.tif5429KSupporting Figure 2. Gnmt ablation increases phosphatidylcholine content in serum HDL fractions. 3-month-old wild type (WT) and Gnmt-/- (KO) mice were fasted 2 hours before experiments were performed. (a) Phosphatidylcholine content in serum lipoprotein fractions was quantified by a commercially available kit (Spinreact, Spain). Lipoprotein fractions were separated by AKTA fast-protein liquid chromatography using a Superose 6TM 10/300 column (GE Healthcare, Sweden). (b) Representative hematoxylin and eosin staining from 3-month-old wild type (WT), Gnmt-/- (KO) and MDD-treated Gnmt-/-(KO-MDD) mice. Values are mean±SEM of 4 animals per group. Statistical differences between Gnmt-/- and WT mice are denoted by *p<0.05; **p<0.01; ***p<0.001 (Student's t test).
hep26399-sup-0003-suppfig3.tif4790KSupporting Figure 3. Gnmt ablation increases hepatic flux from PE to PC in 8 month-old Gnmt-/- mice. 8-month-old wild type (WT) and Gnmt-/- (KO) mice were fasted 2 hours before experiments were performed. (a) Hepatocytes were isolated and incubated with [3H]ethanolamine for 4 hours, and the radioactivity incorporated into PC was expressed as a percentage of the radiolabel incorporated into PC plus PE. Microsomes were isolated from WT and Gnmt-/- mice liver, and PE and PC levels quantified after lipid extraction and separation by TLC. (b) Liver PE and PC were quantified as mentioned before and liver PC/PE ratio was calculated. (c) Liver DG and (d) TG were quantified as mentioned before. (e) Hepatic TG secretion rate was measured after inhibition of VLDL metabolism with 1 g/kg poloxamer (P-407). Values are mean±SEM of 4 animals per group. Statistical differences between Gnmt-/- and WT mice are denoted by *p<0.05; **p<0.01; ***p<0.001 (Student's t test).
hep26399-sup-0004-supptab1.doc314KTable 1 Supporting Lipidomic analysis of livers from Gnmt -/-, Gnmt -/--MDD, and Gnmt -/-Plin2 -/- mice
hep26399-sup-0005-supptab2.doc179KTable 2 Supporting Sequences of the primers used
hep26399-sup-0006-suppinfo.doc89KSupporting Information

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