Potential conflict of interest: Nothing to report.
Autoimmune, Cholestatic and Biliary Disease
Therapeutic advantage of anti-VAP-1 over anti-α4 integrin antibody in concanavalin a-induced hepatitis
Version of Record online: 6 AUG 2013
Copyright © 2013 by the American Association for the Study of Liver Diseases
Volume 58, Issue 4, pages 1413–1423, October 2013
How to Cite
Lee, W.-Y., Salmi, M., Kelly, M. M., Jalkanen, S. and Kubes, P. (2013), Therapeutic advantage of anti-VAP-1 over anti-α4 integrin antibody in concanavalin a-induced hepatitis. Hepatology, 58: 1413–1423. doi: 10.1002/hep.26469
Supported by the Canadian Association of Gastroenterology/Crohn's and Colitis Foundation of Canada Fellowship Award.
- Issue online: 1 OCT 2013
- Version of Record online: 6 AUG 2013
- Accepted manuscript online: 17 MAY 2013 08:39AM EST
- Manuscript Accepted: 11 APR 2013
- Manuscript Received: 16 NOV 2012
- Canadian Association of Gastroenterology/Crohn's and Colitis Foundation of Canada Fellowship Award
- Canadian Institutes of Health Research
- Canada Research Chairs Program and the Alberta Heritage Foundation for Medical Research
Additional Supporting Information may be found in the online version of this article.
|hep26469-sup-0001-suppfig1.tif||14789K||Supporting Fig. 1. Flow cytometric analysis for CD49d+ monocytic myeloid derived supressor cells (MDSCs). Representative dot plots and histograms showing that most of the CD11b+Gr-1dim cells are CD49d+ (upper panel) at 8 h after Con A administration. CD49d+ monocytic MDSCs were gated from mononuclear cells using FSC/SSC dot plots (lower panel).|
|hep26469-sup-0002-suppfig2.tif||2325K||Supporting Fig. 2. Effect of anti-α4 integrin or VAP-1 on serum alanine aminotransferase (ALT) and lung myeloperoxidase (MPO) level. 100 μl of sterile saline (UT), 100 µg of anti-α4 integrin (clone PS/2) or cocktail of 7-88 and 7-106 (50 µg each) were intravenously injected. At 8.5 h after injection, serum and lung tissue were collected for the measurement of ALT and MPO activity, respectively (n = 3-4 per group).|
|hep26469-sup-0003-suppfig3.tif||847K||Supporting Fig. 3. Effects of anti-α4 integrin and anti-VAP-1 on the NK, NKT and CD3 cell recruitment. Con A (15 mg/kg) was administered at 8 h prior to liver isolation and NK (CD3–DX5+), NKT (CD3+DX5+) and CD3 (CD3+DX5–) lymphocytes (B) were counted by flow cytometric analysis (n = 3-6 per group).|
|hep26469-sup-0004-suppfig4.tif||10154K||Supporting Fig. 4. Neutrophil influx into lungs in response to Con A (15 mg/kg) in mice treated with vehicle, anti-α4 integrin or anti-VAP-1 (n = 4-6 per group; **P < 0.01 versus vehicle-treated group by by two-way ANOVA with Bonferroni's correction). MPO, myeloperoxidase.|
|hep26469-sup-0005-suppvideo1.avi||3886K||Supporting Information Video 1|
|hep26469-sup-0006-suppvideo2.avi||6132K||Supporting Information Video 2|
|hep26469-sup-0007-suppvideo3.avi||1078K||Supporting Information Video 3|
|hep26469-sup-0008-suppvideo4.avi||756K||Supporting Information Video 4|
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