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hep26470-sup-0001-suppfig1.eps1068KSupporting Fig.1. Flow cytometry analysis of ADSC surface antigens. The inguinal adipose tissue of GFP-Tg mice was obtained, and the stromal cells were isolated and expanded for six passages in culture. The cells obtained were stained with (A) PE-labeled anti-CD90 or (B) anti-CD105 antibodies followed by flow cytometric analysis.
hep26470-sup-0002-suppfig2.eps1347KSupporting Fig. 2. Albumin expression in hepatic parenchymal cells. ADSCs from GFP-Tg mice (2 × 104) were injected twice every 2 weeks into the splenic subcapsule of cirrhotic C57Bl/6 mice fed the Ath+HF diet for 36 weeks. Control mice received PBS injections. Two weeks after the last injection, liver tissue was obtained and parenchymal cells were isolated for real-time PCR. Expression of (A) albumin and (B) AFP was normalized relative to GAPDH; *P < 0.05.
hep26470-sup-0003-suppfig3.eps4345KSupporting Fig. 3. Effect of ADSCs on collagen IV deposition in the cirrhotic liver. ADSCs from GFP-Tg mice (1 × 105) were injected twice every 2 weeks into the splenic subcapsule of cirrhotic C57Bl/6 mice fed an Ath+HF diet for 32 weeks. Control mice received PBS injections. (A) Two weeks after the last injection, liver tissue was obtained and stained with anti-collagen IV antibody. Scale bars = 100 µm. (B) The stained area was quantified using an image-analysis system. *P< 0.05.
hep26470-sup-0004-suppfig4.eps731KSupporting Fig. 4. Gene expression heat-map related to MMPs. ADSCs from GFP-Tg mice (1 × 105) were injected twice every 2 weeks into the splenic subcapsule of cirrhotic C57Bl/6 mice fed an Ath+HF diet for 40 weeks. Control mice received PBS injections. Two weeks later, liver tissue was subjected to RNA isolation and gene expression was assessed using DNA microarrays. One-way clustering analysis was performed on the obtained data of genes related to MMPs and a heat-map of the analysis is shown.
hep26470-sup-0005-suppfig5.eps2352KSupporting Fig. 5. Gene expression analysis of the CD4+T cell subpopulation of hepatic inflammatory cells. ADSCs from GFP-Tg mice (1 × 105) were injected twice every 2 weeks into the splenic subcapsule of C57Bl/6 mice fed an Ath+HF diet for 12 weeks (n=4). Control mice received PBS injections (n=4). Inflammatory cells were isolated from the liver, followed by separation of CD4+T cells, and gene expression was assessed by real-time PCR. IFN; interferon, TGF; tumor growth factor.
hep26470-sup-0006-supptab1.doc42KTable S1. Chemokine and cytokine-related genes whose expression in the hepatic inflammatory cells of NASH cirrhotic mice was significantly alterd by ADSC treatment.
hep26470-sup-0007-suppinfo.doc27KSupporting Information.

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