Potential conflict of interest: Nothing to report.
Epigenetic regulation of MicroRNA-122 by peroxisome proliferator activated receptor-gamma and hepatitis b virus X protein in hepatocellular carcinoma cells
Version of Record online: 17 SEP 2013
© 2013 by the American Association for the Study of Liver Diseases
Volume 58, Issue 5, pages 1681–1692, November 2013
How to Cite
Song, K., Han, C., Zhang, J., Lu, D., Dash, S., Feitelson, M., Lim, K. and Wu, T. (2013), Epigenetic regulation of MicroRNA-122 by peroxisome proliferator activated receptor-gamma and hepatitis b virus X protein in hepatocellular carcinoma cells. Hepatology, 58: 1681–1692. doi: 10.1002/hep.26514
- Issue online: 30 OCT 2013
- Version of Record online: 17 SEP 2013
- Accepted manuscript online: 23 MAY 2013 04:48PM EST
- Manuscript Accepted: 2 MAY 2013
- Manuscript Revised: 12 MAR 2013
- Manuscript Received: 25 SEP 2012
- National Institutes of Health. Grant Numbers: CA106280, CA102325, CA134568, DK077776 (to T.W.)
Additional Supporting Information may be found in the online version of this article.
|hep26514-sup-0001-suppfig1.tif||3752K||Supporting Figure S1. 5-Aza-CdR/PBA treatment does not alter the levels of HNF4α, C/EBPα, and other related signaling molecules. HepG2 cells were treated with 3 uM 5-Aza-CdR and 3 mM PBA for 48 hours and the cell lysates were obtained for SDS-PAGE and Western blotting analysis using indicated antibodies. (Panel A) Representative western blots for HNF4α, C/EBPα and related molecules. (Panel B) Representative western blots for other signaling molecules.|
|hep26514-sup-0002-suppfig2.tif||2608K||Supporting Figure S2. Representative western blots showing baseline levels of PPARγ, RXRα, N-CoR, SMRT, SUV39H1, and H3K9 di-methyl and tri-methyl histone in human primary hepatocytes (HH) and HCC cell lines (HepG2, Huh7 and Hep3B).|
|hep26514-sup-0003-suppfig3.tif||2360K||Supporting Figure S3. Subcellular localization of HBX and PPARγ in HepG2 cells transiently transfected with HBX expression vector or control vector. The cells were treated with 15-d-PGJ2 or DMSO for 24 hours. Cytosolic and nuclear fractions were obtained for Western blotting using indicated antibodies. Whereas HBX is detected in both cytoplasmic and nuclear fractions, PPARγ is detected only in nuclear fraction. Loading controls include β-tubulin for cytoplasmic protein and PARP for nuclear protein.|
Please note: Wiley Blackwell is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.