Potential conflict of interest: Nothing to report.
CD73 (ecto-5′-nucleotidase) hepatocyte levels differ across mouse strains and contribute to mallory-denk body formation
Article first published online: 26 AUG 2013
© 2013 by the American Association for the Study of Liver Diseases
Volume 58, Issue 5, pages 1790–1800, November 2013
How to Cite
Snider, N. T., Griggs, N. W., Singla, A., Moons, D. S., Weerasinghe, S. V.W., Lok, A. S., Ruan, C., Burant, C. F., Conjeevaram, H. S. and Omary, M. B. (2013), CD73 (ecto-5′-nucleotidase) hepatocyte levels differ across mouse strains and contribute to mallory-denk body formation. Hepatology, 58: 1790–1800. doi: 10.1002/hep.26525
This work was supported by the National Institutes of Health (grant nos.: R01 DK52951 [to M.B.O.] and K01 DK093776 [to N.T.S.]). and Department of Veterans Affairs Merit Review Award (to M.B.O.). This work utilized Core Services (supported by NIH grant nos.: DK089503 and DK034933; to the University of Michigan).
- Issue published online: 30 OCT 2013
- Article first published online: 26 AUG 2013
- Accepted manuscript online: 31 MAY 2013 08:42AM EST
- Manuscript Accepted: 10 MAY 2013
- Manuscript Received: 14 JAN 2013
- The National Institutes of Health. Grant Numbers: R01 DK52951, K01 DK093776
- Department of Veterans Affairs Merit Review Award (to M.B.O.). This work utilized Core Services (supported by grant nos.: DK089503 and DK034933; to the University of Michigan)
Additional Supporting Information may be found in the online version of this article.
|hep26525-sup-0001-suppfig1.tif||12390K||Supplementary Figure 1. CD73 localization in hepatocytes after DDC treatment. Immunofluorescence analysis of Flag-CD73 (green) and different organelle markers (red) in DDC-treated hepatocytes.|
|hep26525-sup-0002-suppfig2.tif||8648K||Supplementary Figure 2. A. Serum analysis for ALT levels in control and DDC-treated WT and CD73-/- mice. B. Liver sections from C3H mice after 3 months DDC treatment were stained with antibodies to CD73 (green) and p62 (red). There is no significant co-localization between the two signals. Scale bars=50μm. C. Liver sections from WT and CD73-/- mice were stained with the designated antibodies. The K8/K18 filament organization is similar between WT and CD73-/- mice under basal conditions. Scale bar=50μm. D. Extensive porphyria (dark brown deposits marked by arrows) is observed in WT and CD73-/- mice after DDC treatment. Also shown is K8/K18 immunofluorescence (red) of the sections. Scale bar=100μm.|
|hep26525-sup-0003-supptable1.tif||586K||Supporting Table 1.|
|hep26525-sup-0004-supptable2.tif||463K||Supporting Table 2.|
|hep26525-sup-0005-supptable3.tif||465K||Supporting Table 3.|
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