Potential conflict of interest: Nothing to report.
Receptor interacting protein kinase 3 is a critical early mediator of acetaminophen-induced hepatocyte necrosis in mice
Article first published online: 11 OCT 2013
© 2013 by the American Association for the Study of Liver Diseases
Volume 58, Issue 6, pages 2099–2108, December 2013
How to Cite
Ramachandran, A., McGill, M. R., Xie, Y., Ni, H.-M., Ding, W.-X. and Jaeschke, H. (2013), Receptor interacting protein kinase 3 is a critical early mediator of acetaminophen-induced hepatocyte necrosis in mice. Hepatology, 58: 2099–2108. doi: 10.1002/hep.26547
Supported in part by National Institutes of Health Grants AA12916 and DK070195 (to H.J.), AA020518 (to WX.D.) and by grants from the National Center for Research Resources (5P20RR021940-07) and the National Institute of General Medical Sciences (8 P20 GM103549-07) from the National Institutes of Health. M.R. McGill was supported by the “Training Program in Environmental Toxicology” (T32 ES007079-26A2) from the National Institute of Environmental Health Sciences.
- Issue published online: 26 NOV 2013
- Article first published online: 11 OCT 2013
- Accepted manuscript online: 6 JUN 2013 04:32PM EST
- Manuscript Accepted: 20 MAY 2013
- Manuscript Received: 2 DEC 2012
Acetaminophen (APAP) overdose is a major cause of hepatotoxicity and acute liver failure in the U.S., but the pathophysiology is incompletely understood. Despite evidence for apoptotic signaling, hepatic cell death after APAP is generally considered necrotic in mice and in humans. Recent findings suggest that the receptor interacting protein kinase 3 (RIP3) acts as a switch from apoptosis to necrosis (programmed necrosis). Thus, the aim of the current investigation was to determine if RIP3 is involved in APAP-induced liver cell death. APAP (200-300 mg/kg) caused glutathione depletion and protein adduct formation, oxidant stress, mitochondrial release of apoptosis inducing factor, and nuclear DNA fragmentation resulting in centrilobular necrosis in C57Bl/6J mice. Inhibiting RIP3 protein induction with antisense morpholinos in wild-type animals or using RIP3-deficient mice had no effect on protein adduct formation but attenuated all other parameters, including necrotic cell death, at 6 hours after APAP. In addition, cultured hepatocytes from RIP3-deficient mice showed reduced injury compared to wild-type cells after 24 hours. Interestingly, APAP-induced mitochondrial translocation of dynamin-related protein 1 (Drp1), the initiator of mitochondrial fission, was inhibited by reduced RIP3 protein expression and the Drp1 inhibitor MDIVI reduced APAP-induced cell death at 24 hours. All of these protective effects were lost after 24 hours in vivo or 48 hours in vitro. Conclusion: RIP3 is an early mediator of APAP hepatotoxicity, involving modulation of mitochondrial dysfunction and oxidant stress. Controlling RIP3 expression could be a promising new approach to reduce APAP-induced liver injury, but requires complementary strategies to control mitochondrial dysfunction for long-term protection. (Hepatology 2013; 58:2099–2108)
apoptosis repressor with caspase recruitment domain
apoptosis signaling-regulating kinase 1
dynamin related protein 1
Fas-associated death domain
hematoxylin & eosin
receptor interacting protein kinase
terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling.
Acetaminophen (APAP) is a safe analgesic and antipyretic drug at therapeutic levels. However, APAP overdose is the most common cause of acute liver failure in the U.S. and U.K. The toxicity is mediated by the metabolism of APAP to the reactive metabolite N-acetyl-p-benzoquinone imine (NAPQI), which forms adducts on cellular proteins, especially in the mitochondria. This leads to mitochondrial oxidant stress, which activates the c-jun N-terminal kinase (JNK), resulting in its translocation to the mitochondria and amplification of the oxidant stress. The extensive mitochondrial oxidant stress eventually causes the opening of the mitochondrial membrane permeability transition pore and necrotic cell death.[6, 7] Early bax translocation to the mitochondria triggers release of intermembrane proteins, including apoptosis-inducing factor (AIF) and endonuclease G, which translocate to the nucleus and are responsible for nuclear DNA fragmentation.[9, 10] Interestingly, smac/DIABLO and cytochrome c are also released but this does not induce caspase activation or apoptotic cell death.[6, 11, 12] The reason for the virtual absence of apoptotic cell death after APAP overdose in mice, in mouse hepatocytes,[5, 7, 13] in a human hepatocyte cell line (HepaRG), and in humans,[15, 16] despite conditions that would support the initiation of a mitochondrial pathway of apoptosis (cytochrome c and smac/DIABLO release), remains unclear. It was argued that declining adenosine triphosphate (ATP) levels due to inhibition of mitochondrial respiration or lack of glycogen in starved mice may prevent apoptosis; however, this explanation may not be sufficient.
Recent data indicate that necrosis can also be a programmed form of cell death (“necroptosis”) and unique molecular pathways involved in necrotic cell death are being defined. The receptor interacting protein kinase 3 (RIP3) has been identified by RNA interference screens as an essential mediator of necrotic cell death induced by tumor necrosis factor alpha (TNF-α) and other ligands.[21, 22] Necrotic signaling by RIP3 requires its interaction with the receptor interacting protein kinase 1 (RIP1) and FADD (Fas-associated death domain), but unlike RIP1, RIP3 is not involved in nuclear factor kappa B (NF-κB) or apoptotic signaling. RIP3 has also been shown to switch TNF-α-induced cell death from apoptosis to necrosis, suggesting that it is a unique molecular regulator that dictates necrotic cell death. Recent studies have shown that downstream cellular signaling induced by TNF-α-mediated activation of RIP3 involves the mixed lineage kinase domain-like protein (MLKL),[24, 25] as well as the mitochondrial phosphatase PGAM5. These mediators in turn have been shown to regulate activation of JNK as well as changes in mitochondrial dynamics. Given the central role of mitochondria and JNK activation in the pathophysiology of APAP-induced necrotic cell death, the objective of the current investigation was to assess a potential role of RIP3 in the pathophysiology of APAP-induced cell death using gene knockdown approaches and genetically deficient mice.
Materials and Methods
Male C57Bl/6J mice (Jackson Laboratories, Bar Harbor, ME) with an average weight of 20 g were used in this study. RIP3-deficient mice (C57Bl/6J background) were kindly provided by Dr. Vishva Dixit (Genentech). All animals were housed in an environmentally controlled room with a 12-hour light/dark cycle and allowed free access to food and water. The experimental protocol was approved by the Institutional Animal Care and Use Committee of the University of Kansas Medical Center and followed the criteria of the National Research Council for the care and use of laboratory animals in research.
In Vivo Morpholino Treatment
All vivo-morpholinos were obtained from Gene Tools (Philomath, OR). Details are described in the Supporting Material.
Mice were injected intraperitoneally with 200 mg/kg or 300 mg/kg APAP (dissolved in warm saline) after overnight fasting. The animals were killed 6 or 24 hours after APAP treatment. Plasma alanine aminotransferase (ALT) activities and liver glutathione (GSH) and glutathione disulfide (GSSG) levels were measured as described in the Supporting Material.
Histology and Immunohistochemistry
Sections of formalin-fixed tissue were stained with hematoxylin and eosin (H&E) for evaluation of necrosis, the terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL) assay for DNA strand breaks, and nitrotyrosine as described in the Supporting Material.
Measurement of APAP Protein Adducts
APAP protein adducts were measured using high-pressure liquid chromatography with electrochemical detection.
Quantitative Real-Time Polymerase Chain Reaction (qRT-PCR)
Expression of the RIP3 gene was quantified using qRT-PCR analysis as described using the following primers: forward: 5′-TCCCAATCTGCACTTCAGAAC-3′, reverse: 5′-GACACGGCACTCCTTGGTAT-3′.
Primary Hepatocyte Isolation
Primary hepatocytes were isolated as described. Cell treatment is described in the Supporting Material.
Isolation of Subcellular Fractions and Western Blotting
Mitochondria and cytosolic fractions were isolated as described. Western blotting was carried out as described in detail using the following antibodies: a rabbit anti-RIP3 antibody (ProSci Incorporated, Poway, CA), a rabbit anti-AIF antibody (Abcam, Cambridge, MA), and a rabbit anti-dynamin-related protein 1 (Drp1) polyclonal antibody (Cell Signaling Technology, Danvers, MA). A horseradish peroxidase-coupled antirabbit IgG (Santa Cruz) was used as secondary antibody.
All results were expressed as mean ± SE. Comparison between two groups were performed with a Student t test. One-way analysis of variance (ANOVA) was used to assess statistical significance between three or more groups. When a difference was detected the Student-Newman-Keul's test was used for multiple comparisons. For nonnormally distributed data the Kruskal-Wallis test was used. P < 0.05 was considered significant.
APAP-Induced RIP3 Expression
To evaluate the role of RIP3 in APAP-induced hepatotoxicity, we began by measuring the time course of RIP3 protein expression after APAP overdose. Treatment of mice with 300 mg/kg APAP revealed a mild response as early as 1 hour with the largest increase between 1 and 3 hours (Fig. 1A,B). Enhanced RIP3 protein levels were also observed after a lower dose of APAP (200 mg/kg) (Fig. 1C,D), which was accompanied by a 6-fold increase in messenger RNA (mRNA) at 6 hours (data not shown).
Functional Significance of RIP3 in APAP Toxicity
To evaluate whether or not the changes in RIP3 protein levels were the cause or merely a consequence of APAP hepatotoxicity, experiments were designed to knock down the protein. Administration of in vivo morpholinos targeting RIP3 resulted in a significant decrease in RIP3 protein in mice (Fig. 1C,D). Although RIP3 morpholinos only partially reduced the expression of constitutive RIP3 protein, it completely prevented RIP3 protein upregulation subsequent to 200 mg/kg APAP at 6 hours. Treatment with control morpholinos did not prevent the APAP-mediated induction of RIP3 (Fig. 1C,D). Measurement of plasma ALT as an indicator of liver cell death demonstrated that treatment with a low overdose of 200 mg/kg APAP resulted in significant liver injury by 6 hours (Fig. 2A). Administration of control morpholinos prior to APAP had no effect on this elevation. However, preventing the up-regulation of RIP3 by treatment with RIP3 morpholinos resulted in a significant protection against APAP-induced liver injury as seen by the dramatically lower ALT values in RIP3 morpholino-treated animals (Fig. 2A). This was supported by histology, where the characteristic centrilobular necrosis after APAP overdose is evident in animals treated with APAP alone or control morpholinos before APAP, while treatment with RIP3 morpholinos resulted in significantly less cellular necrosis (Fig. 2B). In order to assess oxidant stress and peroxynitrite formation, tissue sections were stained for nitrotyrosine (NT) protein adducts (Fig. 2C). Previous studies showed that NT reflects events in mitochondria.[27, 29] APAP caused extensive NT staining in untreated and control morpholino-treated animals. RIP3 morpholinos extensively reduced NT staining, suggesting a substantially lower mitochondrial oxidant stress in these animals (Fig. 2C). Measurement of protein-derived APAP-cysteine adducts showed similar levels of protein binding with or without RIP3 morpholino treatment (Fig. 2D). Thus, the protection of the RIP3 morpholinos against APAP-induced liver injury was not caused by an effect on metabolic activation of APAP.
RIP3 has been suggested to induce mitochondrial generation of free radicals. Mitochondrial oxidant stress followed by activation of the mitochondrial permeability transition and release of mitochondrial proteins such as AIF are known to play critical roles in APAP-induced liver injury. To determine if the APAP-induced induction of RIP3 played a role in mitochondrial dysfunction, the release of AIF into the cytosol was measured (Fig. 3A). As expected, APAP overdose caused AIF release into the cytosol, an effect which was not altered by pretreatment with control morpholinos. Preventing RIP3 induction with the RIP3 morpholinos, however, resulted in almost complete elimination of AIF release into the cytosol (Fig. 3A). This suggests that RIP3 was involved in the mitochondrial dysfunction after APAP overdose. Mitochondrial AIF and endonuclease G are known to cause DNA fragmentation in APAP hepatotoxicity.[9, 10] Consistent with the AIF data (Fig. 3A), RIP3 morpholinos strongly reduced nuclear DNA fragmentation as indicated by the TUNEL assay (Fig. 3B). It has recently been shown that RIP3 controls mitochondrial dynamics by modulating localization of the Drp1, which localizes to mitochondria to induce mitochondrial fission, an event that occurs during cell necrosis. Therefore, mitochondrial Drp1 levels were evaluated by western blotting. APAP treatment induced significant translocation of Drp1 to mitochondria independent of pretreatment with control morpholinos (Fig. 3C). However, mitochondrial Drp1 translocation was completely prevented in animals treated with RIP3 morpholinos.
APAP Toxicity in RIP3-Deficient Mice
In order to support the RIP3 morpholino findings, similar experiments were performed in RIP3-deficient mice using a higher overdose of APAP (300 mg/kg). Treatment of wild-type (RIP3+/+) and RIP3−/− mice with APAP resulted in severe injury in the RIP3+/+ animals as indicated by the increase in plasma ALT activities (Fig. 4A) and extensive necrosis and DNA fragmentation as indicated by the centrilobular TUNEL staining (Fig. 4B). RIP3−/− mice developed substantially less injury as indicated by the 70% reduction in ALT values (Fig. 4A) and the smaller areas of necrosis and less DNA fragmentation (Fig. 4B). Western blot analysis of RIP3 confirmed the induction after APAP treatment in wild-type mice and the lack of the protein in the RIP3−/− animals (Fig. 4C). In addition, the AIF increase in the cytosol in wild-type animals was prevented in RIP3−/− mice (Fig. 4C). Importantly, there was no significant difference in APAP protein adducts, suggesting that the reduced injury in RIP3−/− mice was not caused by inhibition of metabolic activation (Fig. 4D). Hepatic GSH levels, although partially recovered, were still significantly lower than controls in both RIP3+/+ and RIP3−/− mice 6 hours after APAP treatment (Fig. 5A). However, GSSG levels and the GSSG-to-GSH ratio were significantly increased in wild-type animals, reflecting an increased mitochondrial oxidant stress in these livers (Fig. 5B,C). In contrast, GSSG levels in RIP3−/− mice were not different from control values and the GSSG-to-GSH ratio was only modestly elevated, demonstrating significantly less APAP-induced oxidant stress in RIP3-deficient animals (Fig. 5B,C).
Role of RIP3 in Cultured Hepatocytes
To further confirm the protective effect of RIP3-deficiency, cultured hepatocytes isolated from RIP3+/+ and RIP3−/− mice were exposed to 10 mM APAP. As indicated by propidium iodide (PI) staining, there was extensive necrotic cell death in hepatocytes from wild-type mice, which was reduced by 65% in RIP3−/− hepatocytes at 24 hours (Fig. 6A,B). However, the protective effect was lost by 48 hours. Because previous reports indicated a protective effect of the RIP1 inhibitor necrostatin after 20 hours of APAP exposure, we repeated these experiments for up to 48 hours. Similar to the RIP3−/− hepatocytes, necrostatin reduced cell death by 90% at 24 hours but did not protect at 48 hours (Fig. 6C,D). To determine if the effect of RIP3 is more stable in vivo, we assessed liver injury at 24 hours after APAP in RIP3 morpholino-treated animals (200 mg/kg) and in RIP3−/− mice (300 mg/kg). Similar to the observation in cultured cells, the protective effect of RIP3 elimination was lost at 24 hours (Supporting Fig. 1). Likewise, there was no difference in oxidant stress (GSSG) between the groups (data not shown).
Because there is evidence for mitochondrial translocation of Drp1 (Fig. 4), the effect of APAP on mitochondrial morphology was investigated. As shown in Fig. 7A, significant changes were evident in mitochondria 6 hours after APAP treatment. Control cells had a significant number of filamentous mitochondria (Fig. 7A, lower left panel) while almost all mitochondria in APAP-treated cells were fragmented (Fig. 7A, lower right panel). These data suggest that APAP-induced RIP3 induction affects mitochondrial morphology by stimulating Drp1-mediated mitochondrial fission. To test if there is a functional significance to mitochondrial Drp1 translocation, hepatocytes from wild-type mice were treated with 50 μM of the Drp1 inhibitor MDIVI. Compared to control hepatocytes, APAP-induced necrotic cell death in MDIVI-treated cells was reduced by 53% at 24 hours; however, the protective effect of MDIVI was lost at 48 hours (Fig. 7B).
The objective of this investigation was to assess the presence and functional significance of RIP3 in APAP-induced cell death in vitro and liver injury in vivo. Using three different experimental approaches, our data consistently showed that RIP3 is a critical component of the signaling cascade of APAP-induced cell death independent of the dose, at least during the early injury phase.
RIP3 Expression and APAP-Induced Cell Death
RIP3 protein expression in livers of untreated C57Bl/6J mice and hepatocytes was low, but was significantly induced after APAP treatment. RIP3 morpholinos were only modestly effective in reducing the baseline levels of RIP3 but prevented APAP-induced transcriptional activation of RIP3. In contrast to control morpholinos, which did not affect RIP3 induction or APAP-induced liver injury, RIP3 morpholinos were highly effective in reducing APAP-induced cell death. These data suggest that the early transcriptional induction of RIP3 is critical for APAP-induced necrosis. The importance of RIP3 in APAP-induced liver injury was confirmed in RIP3-deficient mice and in cultured hepatocytes, suggesting that RIP3 expression in parenchymal cells is involved in cell death. The mode of cell death after APAP is generally considered to be necrosis due to characteristics such as cell swelling and massive cell contents release, the absence of active caspases, and the lack of a protective effect of caspase inhibitors in this model.[6, 7, 11, 12, 17] The current findings that RIP3 is critical for the injury combined with recent reports on the beneficial effect of the RIP1 inhibitor necrostatin[30, 32] indicate that early APAP-induced hepatocyte cell death fits many characteristics of programmed necrosis (necroptosis). Interestingly, in the absence of RIP3 the remaining cell death is still necrotic based on the TUNEL assay, which shows the typical features of necrotic DNA fragmentation. Although the most studied initiator of necroptosis is TNF-α and TNF receptor 1 signaling, TNF-α is unlikely the initiator in the context of APAP hepatotoxicity. First, there is very limited TNF-α protein formation in response to APAP overdose.[33, 34] Second, TNF-deficient or TNF receptor type 1-deficient mice are not protected against APAP hepatotoxicity[35, 36] and anti-TNF-α antibodies or soluble TNF-α receptor treatment did not affect APAP-induced liver injury. Third, treatment with a low dose of endotoxin, which produces large amounts of TNF-α, did not enhance APAP-induced liver injury. Together, these data suggest that TNF receptor 1 signaling is probably not responsible for the formation of the necrosome and initiation of programmed necrosis during APAP hepatotoxicity. Although TNF receptor signaling is the best-studied initiating event for necroptosis, it is well established that there are multiple ways to trigger this mode of cell death. Further studies are needed to identify potential activators of this pathway in APAP hepatotoxicity.
Downstream Events of RIP3 Activation
A critical question is what downstream events are triggered by RIP3 activation, which may lead to cell death. Mitochondrial dysfunction has emerged as the central and most critical event in the pathophysiology of APAP toxicity in experimental animals and humans. Inhibition of respiration, a selective mitochondrial oxidant stress, mitochondrial DNA damage, nuclear DNA fragmentation dependent on mitochondrial dysfunction, and eventual mitochondrial permeability transition (MPT) pore opening with collapse of the membrane potential and ATP depletion are all hallmarks of APAP-induced cell death. Our current data indicate that the absence of RIP3 or prevention of RIP3 induction can eliminate mitochondrial AIF release and attenuate nuclear DNA fragmentation and the oxidant stress which is almost exclusively located in the mitochondria. Recent insight into the development of the mitochondrial oxidant stress suggests the presence of an amplification loop. The initial metabolic stress, presumably protein adducts in mitochondria, induces a moderate mitochondrial oxidant stress, which triggers JNK phosphorylation through activation of MLK3 and apoptosis signal-regulating kinase 1 (ASK1). P-JNK translocates to the mitochondria and potentiates the oxidant stress, which eventually triggers the MPT and cell necrosis.[5, 40] The fact that RIP3-deficiency prevented mitochondrial dysfunction and oxidant stress suggests that RIP3 acted upstream of JNK activation. Consistent with this conclusion is the observation that the RIP1 inhibitor necrostatin reduced APAP-induced liver injury by inhibiting JNK activation. In addition, overexpression of ARC (apoptosis repressor with caspase recruitment domain), which interacts with JNK downstream of the necrosome, attenuated JNK activation, oxidant stress, and liver cell injury. Thus, the emerging evidence from our data in combination with recently published findings strongly suggests that RIP3 is involved in regulating the mitochondrial oxidant stress through JNK activation.
In addition to modulation of the APAP-induced oxidant stress by RIP3, we observed the mitochondrial translocation of Drp1, which is a protein involved in mitochondrial fission. Mitochondria are now recognized to be dynamic organelles, which undergo continuous cycles of fusion and fission to maintain function. Mitochondrial fission has been shown to occur during cell death, predominantly in models of apoptosis. It was recently shown that RIP3-mediated necrotic cell death can also occur through activation of mitochondrial fission. The molecular control of mitochondrial fission is mediated by Drp1, which polymerizes and constricts mitochondria to facilitate organelle division. Our data provide evidence that mitochondrial fission seems to be a feature of APAP-induced hepatocyte necrosis and this is controlled by RIP3-mediated Drp1 translocation to the mitochondria. The Drp1 inhibitor MDIVI prevented cell death, suggesting that Drp1 translocation and mitochondrial fission are critical events in APAP-induced cell death. Mitochondrial fission has been associated with production of reactive oxygen species (ROS). Thus, RIP3 can affect the critical mitochondrial oxidant stress by controlling JNK activation and mitochondrial fission during APAP hepatotoxicity.
Despite convincing evidence for the involvement of RIP3 in the signaling mechanisms of cell death early after APAP (6 hours in vivo and 24 hours in vitro), the protective effect of RIP3 elimination was lost at later timepoints (24 hours in vivo and 48 hours in vitro). Interestingly, the recently reported protection by the RIP1 inhibitor necrostatin was confirmed in our study; however, this protection was also lost at 48 hours in vitro, as was the reduced cell death with the Drp1 inhibitor MDIVI. Although it is beyond the scope of the present study to elucidate the mechanistic details of this effect, there are some similarities to our previous findings on the role of mitochondrial bax translocation in APAP hepatotoxicity. Bax deficiency was found to prevent the outer mitochondrial membrane permeability, DNA fragmentation, and cell necrosis at 6 hours. However, this effect was lost by 12 hours because of continued mitochondrial oxidant stress, which eventually became the dominant injury mechanism. Our data suggest that the initiation of cell death by RIP3-dominant programmed necrosis signaling is eventually overtaken by a secondary event. The fact that protein adduct formation is unaffected by RIP3 but the downstream mitochondrial dysfunction and oxidant stress is drastically reduced indicates that RIP3 is one factor that controls JNK activation and the mitochondrial oxidant stress. However, if this pathway is eliminated, the effect on mitochondria is delayed but not prevented. More studies are needed to elucidate the delicate network of mitogen-activated protein kinase (MAPK) regulation of the mitochondrial oxidant stress, which may be the ultimate deciding factor in cell death after APAP overdose.
In conclusion, our data identified a unique molecular mediator for early APAP-induced hepatocyte necrosis, namely RIP3. APAP overdose triggers the transcriptional activation of RIP3 and the newly expressed RIP3 is critical for cell necrosis by acting upstream of mitochondrial dysfunction and oxidant stress, which is controlled by JNK activation and mitochondrial translocation of P-JNK. We have also shown that mitochondrial dynamics are relevant to the mechanism of APAP-induced cell death and are controlled by activation of RIP3, which induces translocation of Drp1 from the cytosol to the mitochondria. The resulting mitochondrial fission and oxidant stress lead to activation of the mitochondrial permeability transition, release of AIF, nuclear DNA fragmentation, and hepatocyte necrosis. However, despite its prominent role in the cell death signaling pathway, elimination of RIP3 does not cause long-term protection, most likely due to alternate pathways of amplification of the mitochondrial oxidant stress. Controlling RIP3 induction or modulating function could be a promising new therapeutic approach to prevent the early APAP-induced liver injury, but requires complementary strategies to control the mitochondrial dysfunction for long-term protection.
- 36Tumour necrosis factor receptor 1 and hepatocyte regeneration in acetaminophen toxicity: a kinetic study of proliferating cell nuclear antigen and cytokine expression. Basic Clin Pharmacol Toxicol 2005;97:8-14., , , , .
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|hep26547-sup-0003-suppFig1.tif||2535K||Supporting Information Figure 1|
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