Potential conflict of interest: Nothing to declare.
Human serum leads to differentiation of human hepatoma cells, restoration of very-low-density lipoprotein secretion, and a 1000-fold increase in HCV Japanese fulminant hepatitis type 1 titers
Article first published online: 17 OCT 2013
© 2013 by the American Association for the Study of Liver Diseases
Volume 58, Issue 6, pages 1907–1917, December 2013
How to Cite
Steenbergen, R. H.G., Joyce, M. A., Thomas, B. S., Jones, D., Law, J., Russell, R., Houghton, M. and Tyrrell, D. L. (2013), Human serum leads to differentiation of human hepatoma cells, restoration of very-low-density lipoprotein secretion, and a 1000-fold increase in HCV Japanese fulminant hepatitis type 1 titers. Hepatology, 58: 1907–1917. doi: 10.1002/hep.26566
The authors thank Dr. Rice for the kind gift of Huh7.5 cells and Dr. Wakita for the JFH-1 construct. This research was funded by the Li Ka Shing Foundation, Canadian Institutes for Health Research, and Alberta Advanced Education and Technology (AAET).
- Issue published online: 26 NOV 2013
- Article first published online: 17 OCT 2013
- Accepted manuscript online: 14 JUN 2013 09:41AM EST
- Manuscript Accepted: 27 MAY 2013
- Manuscript Received: 30 JAN 2013
Additional Supporting Information may be found in the online version of this article.
Supplemental figure 1.
Comparison to DMSO and Adult bovine serum mediated growth arrest Historically, fetal serum has been used in cell cultures because of the presence of high levels of growth stimulating factors. We wanted to determine if some of the same effects seen in HS (adult human serum) could be achieved by switching FBS to ABS (adult bovine serum). Also, HuH-7 or HuH-7-derived cells become contact inhibited by DMSO, as reported previously (4). We therefore characterized Huh7.5 cells grown in either FBS media further supplemented with DMSO (1%) or in media supplemented with adult bovine serum (ABS, 2%).
Both ABS and DMSO supplementation resulted in growth arrest and changes in cellular morphology, however these changes were less pronounced than in HS; for example the increase in cells size that was observed under HS conditions was not observed in DMSO or ABS containing medium. The degree of differentiation was further examined by quantitative RT-PCR for differentiation markers, cell-cell contact components and key lipid regulators, and compared with cells cultured in HS supplemented media (supplemental figure 1). Of the hepatocyte specific markers, only albumin was increased in DMSO, but not in ABS, and did not reach the level found in HS media. However, DMSO supplementation also resulted in a decrease in LDL-R transcripts. (suppl. figure 1A). Of the cell-cell contact markers, only occludin was increased by supplementation with ABS, and again not to levels found in HS media (suppl. figure 1B). The levels of key lipid regulators were not affected by culturing in DMSO or ABS, with the exception that LXRα was significantly decreased in DMSO cultured cells (suppl. figure 1C). In line with this, the increase in lipid droplet content that was observed in HS was not seen in cells cultured in either DMSO or in ABS (not shown).
Summarizing, although both DMSO and ABS induce growth arrest and some of the same morphological changes as seen in HS, and results in expression of some differentiation markers, neither of them induces all changes, nor at similar levels that HS supplementation does.
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