Potential conflict of interest: Nothing to report.
Isolate-dependent use of claudins for cell entry by hepatitis C virus
Version of Record online: 8 NOV 2013
© 2013 by the American Association for the Study of Liver Diseases
Volume 59, Issue 1, pages 24–34, January 2014
How to Cite
Haid, S., Grethe, C., Dill, M. T., Heim, M., Kaderali, L. and Pietschmann, T. (2014), Isolate-dependent use of claudins for cell entry by hepatitis C virus. Hepatology, 59: 24–34. doi: 10.1002/hep.26567
This work was supported by a grant from the Helmholtz Association (SO-024; to T.P.). The authors are grateful to Takaji Wakita for the gift of the JFH1 isolate, to Jens Bukh for the different HCV chimeras, to Charles Rice for Huh-7.5 cells and 9E10 antibody, to Brent E. Korba for providing HuH6 cells, and to Matthew Evans for the miR122 expression construct. The authors are grateful to Isidro Hötzel and GENENTCH for providing the CLDN1-specific monoclonal antibody.
- Issue online: 20 DEC 2013
- Version of Record online: 8 NOV 2013
- Accepted manuscript online: 14 JUN 2013 09:43AM EST
- Manuscript Accepted: 28 MAY 2013
- Manuscript Received: 11 APR 2013
Additional Supporting Information may be found in the online version of this article.
|hep26567-sup-0002-suppfig1.tif||333K||Supplementary Figure 1: Differences in susceptibility to HCV infection of Huh-7.5 compared to HuH6 cells. The susceptibility of Huh-7.5 and HuH6 cells to HCVcc particles was calculated. To this end, TCID50 titers of given HCVcc particles determined in Huh-7.5 cells were divided by the titer observed after inoculation of HuH6 cells with the same virus stock. Mean values and standard deviation of four independent experiments are given.|
|hep26567-sup-0003-suppfig2.tif||1010K||Supplementary Figure 2: CLDN6 expression in human liver biopsies. The CLDN6 expression in human liver biopsies was analyzed in regard to (A) HCV genotype of the infected patient, (B) fibrosis stage rated in METAVIR F groups, (C) disease duration and (D) gender of the patients.|
|hep26567-sup-0004-supptable1.tif||1015K||Supplementary Table 1: Data of patients included in this study.|
|hep26567-sup-0005-supptable2.tif||995K||Supplementary Table 2: SiRNA sequences used for knock-down of HCV entry receptors.|
|hep26567-sup-0006-supptable3.tif||795K||Supplementary Table 3: Primers and Probes used for qRT-PCR. mRNA specific qRT-PCR primers and probes used for expression analysis of HCV entry receptors. qRT-PCR analysis was performed using a one–step RT-PCR LightCycler 480 RNA Master Hydrolysis Probes Kit (Roche, Mannheim, Germany) and a LightCycler 480 machine (Roche).|
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