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Additional Supporting Information may be found in the online version of this article.

FilenameFormatSizeDescription
hep26662-sup-0001-suppfig1.tif1097KSupporting Information Figure 1. Doxycycline-regulated expression of MYC or/and RAS oncogenes give rise to distinct liver tumors. A. Western blot confirming the expression of MYC and RAS in oncogene driven liver tumors. B. Table summarizing the histological characteristics of tumors driven by respective oncogenes.
hep26662-sup-0002-suppfig2.tif402KSupporting Information Figure 2. Genomic organization of the Dlk1-Dio3 locus showing location of representative miRNAs
hep26662-sup-0003-suppfig3.tif1009KSupporting Information Figure 3. A. Schematic of miRNA subcluster cloning from the Dlk1-Dio3 region. B. Western blot showing expression of MYC and RAS in LT2M and LT2MR cell lines. C. Verification of miRNA subscluster expression in LT2MR stable cell lines. qRT-PCR was performed to detect expression of representative miRNAs.
hep26662-sup-0004-suppfig4.tif324KSupporting Information Figure 4. Verification of miR-494 and 495 expression in mouse liver tumors. qRT-PCR was performed to detect expression of respective miRNAs.
hep26662-sup-0005-suppfig5.tif329KSupporting Information Figure 5. Quantification of miR-494 levels in engineered LT2MR cell lines by qRT-PCR.
hep26662-sup-0006-suppfig6.tif553KSupporting Information Figure 6. A. Quantification of miR-494 expression in Hepa1-6 cell lines transfected with either a control or miR-494 antimiR, by qRT-PCR. B. Proliferation assay performed on Hepa1-6 cells transfected with either control or miR-494 antimiR. C. Cell cycle analysis following staining for DNA content performed on Hepa1-6 cells transfected with either control or miR-494 antimiR. D. Western blot analysis of MCC, p27 and p21 expression of Hepa1-6 cells cells transfected with either control or miR-494 antimiR.
hep26662-sup-0007-suppfig7.tif355KSupporting Information Figure 7. Luciferase reporter assay on TACC2, TGFB2, RB1, RB1CC1, CDC73 and WEE1 3′ UTRs.
hep26662-sup-0008-suppfig8.tif3166KSupporting Information Figure 8. A. Quantification of miR-494 levels in Huh7 and Hep3B cells relative to normal liver tissue. B. Verification of miR-494 expression in tumors treated with either control or miR-494 antimiR. miR-451 expression was tested as a negative control. qRT-PCR was performed to detect expression of respective miRNAs. C. Quantification of TUNEL staining performed on control and miR-494 antimiR treated animals. Representative pictures are shown.
hep26662-sup-0009-supptable1.doc547KSupporting Information Table 1. Differential expression of miRNAs (fold change -1 > log2 >1 and p<0.05) sorted by tumor specific expression in transgenic mouse tumor models driven by MYC, RAS or MYC+RAS. miRNAs that are upregulated, downregulated and a part of the 12q cluster are listed in separate worksheets for all three genotypes.
hep26662-sup-0010-supptable2.docx36KSupporting Information Table 2. Increased expression of miR-494 and miR-495 in transgenic mouse liver tumors. Values are expressed as fold difference compared to normal LT2 liver tissue.
hep26662-sup-0011-supptable3.doc13KSupporting Information Table 3. Primer sequences used for cloning of 12q subcluster miRNAs and 3′UTR reporter constructs.
hep26662-sup-0012-suppinfo.docx112KSupporting Information

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