• 1
    Choo QL, Richman KH, Han JH, Berger K, Lee C, Dong C, Gallegos C, et al. Genetic organization and diversity of the hepatitis C virus. Proc Natl Acad Sci U S A 1991; 88: 24512455.
  • 2
    Kato N, Hijikata M, Ootsuyama Y, Nakagawa M, Ohkoshi S, Sugimura T, Shimotohno K. Molecular cloning of the human hepatitis C virus genome from Japanese patients with non-A, non-B hepatitis. Proc Natl Acad Sci USA 1990; 87: 95249528.
  • 3
    Heinz FX. Comparative molecular biology of flaviviruses and hepatitis C virus. Arch Virol 1992; 4: 163171.
  • 4
    Weiner AJ, Brauer MJ, Rosenblatt J, Richman KH, Tung J, Crawford K, Bonino F, et al. Variable and hypervariable domains are found in the regions of HCV corresponding to the flavivirus envelope and NS1 proteins and the pestivirus envelope glycoproteins. Virology 1991; 180: 842848.
  • 5
    Han JH, Shyamala V, Richman KH, Brauer MJ, Irvine B, Urdea MS, Tekamp-Olson P, et al. Characterization of the terminal regions of hepatitis C viral RNA: identification of conserved sequences in the 5′ untranslated region and poly(A) tails at the 3′ end. Proc Natl Acad Sci USA 1991; 88: 17111715.
  • 6
    Okamoto H, Okada S, Sugiyama Y, Yotsumoto S, Tanaka T, Yoshizawa H, Tsuda F, et al. The 5′-terminal sequence of the hepatitis C virus genome. Japanese J Exp Med 1990; 60: 167177.
  • 7
    Takeuchi K, Kubo Y, Boonmar S, Watanabe Y, Katayama T, Choo QL, Kuo G, et al. The putative nucleocapsid and envelope protein genes of hepatitis C virus determined by comparison of the nucleotide sequences of two isolates derived from an experimentally infected chimpanzee and healthy human carriers. J Gen Virol 1990; 71: 30273033.
  • 8
    Takeuchi K, Kubo Y, Boonmar S, Watanabe Y, Katayama T, Choo QL, Kuo G, et al. Nucleotide sequence of core and envelope genes of the hepatitis C virus genome derived directly from human healthy carriers. Nuc Acids Res 1990; 18: 4626.
  • 9
    Lesniewski RR, Boardway KM, Casey JM, Desai SM, Devare SG, Leung TK, Mushahwar IK. Hypervariable 5′-terminus of hepatitis C virus E2/ NS1 encodes antigenically distinct variants. J Med Virol 1993; 40: 150156.
  • 10
    Okamoto H, Kojima M, Okada S, Yoshizawa H, Lizuka H, Tanaka T, Muchmore EE, et al. Genetic drift of hepatitis C virus during an 8.2-year infection in a chimpanzee: variability and stability. Virology 1992; 190: 894899.
  • 11
    Cuthbert JA. Hepatitis C: progress and problems. Clin Microb Rev 1994; 7: 505532.
  • 12
    Shiratori Y, Shiina S, Imamura M, Kato N, Kanai F, Okudaira T, Teratani T, et al. Characteristic difference of hepatocellular carcinoma between hepatitis B- and C-viral infection in Japan. Hepatology 1995; 22: 10271033.
  • 13
    Chisari FV, Klopchin K, Moriyama T, Pasquinelli C, Dunsford HA, Sell S, Pinkert CA, et al. Molecular pathogenesis of hepatocellular carcinoma in hepatitis B virus transgenic mice. Cell 1989; 59: 11451156.
  • 14
    Chisari FV, Ferrari C. Hepatitis B virus immunopathogenesis. Ann Rev Immunol 1995; 13: 2960.
  • 15
    Held WA, Mullins JJ, Kuhn NJ, Gallagher JF, Gu GD, Gross KW. T antigen expression and tumorigenesis in transgenic mice containing a mouse major urinary protein/SV40 T antigen hybrid gene. EMBO J 1989; 8: 183191.
  • 16
    Shiota G, Rhoads DR, Wang TC, Nakamura T, Schmidt EV. Hepatocyte growth factor inhibits growth of hepatocellular carcinoma cells. Proc Natl Acad Sci U S A 1992; 89: 373377.
  • 17
    Spaete RR, Alexander D, Rugroden ME, Choo QL, Berger K, Crawford K, Kuo C, et al. Characterization of the hepatitis C virus E2/NS1 gene product expressed in mammalian cells. Virology 1992; 188: 819830.
  • 18
    Mizushima H, Hijikata M, Asabe S, Hirota M, Kimura K, Shimotohno K. Two hepatitis C virus glycoprotein E2 products with different C termini. J Virol 1994; 68: 62156222.
  • 19
    Dubuisson J, Hsu HH, Cheung RC, Greenberg HB, Russell DG, Rice CM. Formation and intracellular localization of hepatitis C virus envelope glycoprotein complexes expressed by recombinant vaccinia and Sindbis viruses. J Virol 1994; 68: 61476160.
  • 20
    Hogan B, Constantini F, Lacy E. Manipulating the Mouse Embryo: A Laboratory Manual. Cold Spring Harbor Laboratory Press, 1994.
  • 21
    Chirgwin JM, Przybyla AE, MacDonald RJ, Rutter WJ. Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease activity. Biochemistry 1979; 18: 52945299.
  • 22
    Moradpour D, Wakita T, Tokushige K, Carlson R, Krawczynski K, Wands JR. Characterization of three novel monoclonal antibodies against hepatitis C virus core protein. J Med Virol 1996; 48: 234241.
  • 23
    Lesniewski R, Okasinski G, Carrick R, Van Sant C, Desai S, Johnson R, Scheffel J, et al. Antibody to hepatitis C virus second envelope (HCV–E2) glycoprotein: a new marker of HCV infection closely associated with viremia. J Med Virol 1995; 45: 415422.
  • 24
    Baumert, Liang TJ. 1996; in press.
  • 25
    Lemon SM, Brown EA. Hepatitis C virus and chronic liver disease. Curr Clin Top Infect Dis 1994; 14: 120141.
  • 26
    Hsia PC, Seeff LB. Non-A, non-B hepatitis: impact of the emergence of the hepatitis C virus. Adv Int Med 1992; 37: 197222.
  • 27
    Houghton M, Selby M, Weiner A, Choo QL. Hepatitis C virus: structure, protein products and processing of the polyprotein precursor. Curr Studies Hem and Blood Trans 1994; 61: 111.
  • 28
    Omata M, Kato N. Recent advances in hepatitis C virus research. J Gas-troenterol 1994; 29: 377382.
  • 29
    Choo QL, Kuo G, Weiner AJ, Overby LR, Bradley DW, Houghton M. Isolation of a cDNA clone derived from a blood-borne non-A, non-B viral hepatitis genome. Science 1989; 244: 359362.
  • 30
    Shimizu YK, Hijikata M, Iwamoto A, Alter HJ, Purcell RH, Yoshikura H. Neutralizing antibodies against hepatitis C virus and the emergence of neutralization escape mutant viruses. J Virol 1994; 68: 14941500.
  • 31
    Farci P, Alter HJ, Govindarajan S, Wong DC, Engle R, Lesniewski RR, Mushahwar IK, et al. Lack of protective immunity against reinfection with hepatitis C virus. Science 1992; 258: 135140.
  • 32
    Prince AM, Brotman B, Huima T, Pascual D, Jaffery M, Inchauspe G. Immunity in hepatitis C infection. J Infect Dis 1992; 165: 438443.
  • 33
    Koziel MJ, Dudley D, Wong JT, Dienstag J, Houghton M, Ralston R, Walker BD. Intrahepatic cytotoxic T lymphocytes specific for hepatitis C virus in persons with chronic hepatitis. J Immunol 1992; 149: 33393344.
  • 34
    Koziel MJ, Dudley D, Afdhal N, Choo QL, Houghton M, Ralston R, Walker BD. Hepatitis C virus (HCV)-specific cytotoxic T lymphocytes recognize epitopes in the core and envelope proteins of HCV. J Virol 1993; 67: 75227532.
  • 35
    Erickson AL, Houghton M, Choo QL, Weiner AJ, Ralston R, Muchmore E, Walker CM. Hepatitis C virus-specific CTL responses in the liver of chimpanzees with acute and chronic hepatitis C. J Immunol 1993; 151: 41894199.
  • 36
    Botarelli P, Brunetto MR, Minutello MA, Calvo P, Unutmaz D, Weiner AJ, Choo QL, et al. T-lymphocyte response to hepatitis C virus in different clinical courses of infection. Gastroenterology 1993; 104: 580587.
  • 37
    Sallie R, Di Bisceglie AM. Viral hepatitis and hepatocellular carcinoma. Gastroent Clin N Amer 1994; 23: 567579.
  • 38
    Liang TJ, Jeffers LJ, Reddy KR, De Medina M, Parker IT, Cheinquer H, Idrovo V, et al. Viral pathogenesis of hepatocellular carcinoma in the United States. Hepatology 1993; 18: 13261333.
  • 39
    De Mitri MS, Poussin K, Baccarini P, Pontisso P, A DE, Simon N, Grigioni W, et al. HCV-associated liver cancer without cirrhosis. Lancet 1995; 345: 413415.
  • 40
    Sakamuro D, Furukawa T, Takegami T. Hepatitis C virus nonstructural protein NS3 transforms NIH 3T3 cells. J Virol 1995; 69: 38933896.
  • 41
    Shih CM, Chen CM, Chen SY, Lee YH. Modulation of the trans-suppression activity of hepatitis C virus core protein by phosphorylation. J Virol 1995; 69: 11601171.
  • 42
    Chisari FV. Hepatitis B virus transgenic mice: insights into the virus and the disease. Hepatology 1995; 22: 13161325.
  • 43
    Koike K, Moriya K, Ishibashi K, Masuura Y, Suzuki T, Saito I, Iino S, et al. Expression of hepatitis C virus envelope proteins in transgenic mice. J Gen Virol 1995; 76: 30313038.
  • 44
    Shimizu YK, Feinstone SM, Kohara M, Purcell RH, Yoshikura H. Hepatitis C virus: detection of intracellular virus particles by electron microscopy. Hepatology 1996; 23: 205209.
  • 45
    Ralston R, Thudium K, Berger K, Kuo C, Gervase B, Hall J, Selby M, et al. Characterization of hepatitis C virus envelope glycoprotein complexes expressed by recombinant vaccinia viruses. J Virol 1993; 67: 67536761.
  • 46
    Santolini E, Migliaccio G, La Monica N. Biosynthesis and biochemical properties of the hepatitis C virus core protein. J Virol 1994; 68: 36313641.
  • 47
    Moriyama T, Guilhot S, Klopchin K, Moss B, Pinkert CA, Palmiter RD, Brinster RL, et al. Immunobiology and pathogenesis of hepatocellular injury in hepatitis B virus transgenic mice. Science 1990; 248: 361364.