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Different hepatitis b virus genotypes are associated with different mutations in the core promoter and precore regions during hepatitis B e antigen seroconversion

  1. Henry L. Y. Chan†,
  2. Munira Hussain,
  3. Anna S. F. Lok M.D.*

Article first published online: 30 DEC 2003

DOI: 10.1002/hep.510290352

Issue

Hepatology

Hepatology

Volume 29, Issue 3, pages 976–984, March 1999

Additional Information

How to Cite

Chan, H. L. Y., Hussain, M. and Lok, A. S. F. (1999), Different hepatitis b virus genotypes are associated with different mutations in the core promoter and precore regions during hepatitis B e antigen seroconversion. Hepatology, 29: 976–984. doi: 10.1002/hep.510290352

Author Information

  1. Division of Gastroenterology, University of Michigan and VA Medical Center, Ann Arbor, MI

  1. †

    Department of Medicine, Prince of Wales Hospital, Shatin, Hong Kong, China

*Division of Gastroenterology, University of Michigan Medical Center, 3912 Taubman Center, Box 0362, Ann Arbor, MI 48109. fax: (734) 936-7392

Publication History

  1. Issue published online: 30 DEC 2003
  2. Article first published online: 30 DEC 2003
  3. Manuscript Accepted: 30 NOV 1998
  4. Manuscript Received: 12 AUG 1998

Funded by

  • Hong Kong Association for the Study of Liver Diseases (to Dr. Chan) and a VA Merit Review Award (to Dr. Lok)

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References

  • 1
    Akahane Y, Yamanaka T, Suzuki H, Sugai Y, Tsuda F, Yotsumoto S, Omi S, et al. Chronic active hepatitis with hepatitis B virus DNA and antibody against e antigen in the serum. Disturbed synthesis and secretion of e antigen from hepatocytes due to a point mutation in the precore region. Gastroenterology 1990; 99:1113–1119.MEDLINE
  • 2
    Brunetto MR, Giarin MM, Oliveri F, Chiaberge E, Baldi M, Alfarano A, Serra A, et al. Wild-type and e antigen–minus hepatitis B viruses and course of chronic hepatitis. Proc Natl Acad Sci U S A 1991; 88:4186–4190.MEDLINE
  • 3
    Carman WF, Hadziyannis S, McGarvey MJ, Jacyna MR, Karayiannis P, Makris A, Thomas HC. Mutation preventing formation of hepatitis B e antigen in patients with chronic hepatitis B infection. Lancet 1989; ii:588–590.
  • 4
    Lok ASF, Akarca U, Greene S. Mutations in the pre-core region of hepatitits B virus serve to enhance the stability of the secondary structure of the pre-genome encapsidation signal. Proc Natl Acad Sci U S A 1994; 91:4077–4081.MEDLINE
  • 5
    Okamoto H, Yotsumoto S, Akahane Y, Yamanaka T, Miyazaki Y, Sugai Y, Tsuda F, et al. Hepatitis B viruses with precore region defects prevail in persistently infected hosts along with seroconversion to the antibody against e antigen. J Virol 1990; 64:1298–1303.MEDLINE
  • 6
    Takeda K, Akahane Y, Suzuki H, Okamoto H, Tsuda F, Miyakawa Y, Mayumi M. Defects in the precore region of the HBV genome in patients with chronic hepatitis B after sustained seroconversion from HBeAg to anti-HBe induced spontaneously or with interferon therapy. Hepatology 1990; 12:1284–1289.MEDLINE
    Direct Link:
  • 7
    Hamasaki K, Nakata K, Nagayama Y, Ohtsuru A, Daikoku M, Taniguchi K, Tsutsumi T, et al. Changes in the prevalence of HBeAg-negative mutants hepatitis B virus during the course of chronic hepatitis B. Hepatology 1994; 20:8–14.MEDLINE
    Direct Link:
  • 8
    Li JS, Tong SP, Wen YM, Vitvitski L, Zhang Q, Trepo C. Hepatitis B virus genotype A rarely circulates as an HBe-minus mutant: possible contribution of a single nucleotide in the precore region. J Virol 1993; 67:5402–5410.MEDLINE
  • 9
    Rodriguez-Frias F, Buti M, Jardi R, Cotrina M, Viladomiu L, Esteban R, Guardia J. Hepatitis B virus infection: precore mutants and its relation to viral genotypes and core mutations. Hepatology 1995; 22:1641–1647.MEDLINE
    Direct Link:
  • 10
    Lindh M, Andersson AS, Gusdal A. Genotypes, nt 1858 variants, and geographic origin of hepatitis B virus—large-scaled analysis using a new genotyping method. J Infect Dis 1997; 175:1285–1293.MEDLINE
  • 11
    Lok ASF, Akarca US, Greene S. Predictive value of precore hepatitis B virus mutations in spontaneous and interferon-induced hepatitis B e antigen clearance. Hepatology 1995; 21:19–24.MEDLINE
  • 12
    Okamoto H, Tsuda F, Akahane Y, Sugai Y, Yoshiba M, Moriyama K, Tanaka T, et al. Hepatitis B virus with mutations in the core promoter for an e antigen-negative phenotype in carriers with antibody to e antigen. J Virol 1994; 68:8102–8110.MEDLINE
  • 13
    Kidd-Ljunggren K, Oberg M, Kidd AH. Hepatitis B virus X gene 1751 to 1764 mutations: implications for HBeAg status and disease. J Gen Virol 1997; 78:1469–1478.MEDLINE
  • 14
    Kurosaki M, Enomoto N, Asahina Y, Sakuma I, Ikeda T, Tozuka S, Izumi N, et al. Mutations in the core promoter region of hepatitis B virus in patients with chronic hepatitis B. J Med Virol 1996; 49:115–123.MEDLINE
    Direct Link:
  • 15
    Horikata M, Itoh V, Yamamoto K, Shibayama T, Tsuda F, Okamoto H. Differences in the entire nucleotide sequence between hepatitis B virus genomes from carriers positive for antibody to hepatitis B e antigen with and without active disease. J Med Virol 1994; 44:96–103.MEDLINE
    Direct Link:
  • 16
    Buckwold VE, Xu Z, Chen M, Yen TSB, Ou JH. Effects of a naturally occurring mutation in the hepatitis virus basal core promoter on precore gene expression and viral replication. J Virol 1996; 70:5845–5851.MEDLINE
  • 17
    Scaglioni PP, Melegari M, Wands JR. Biological properties of hepatitis B viral genomes with mutations in the precore promoter and precore open reading frame. Virology 1997; 233:374–381.MEDLINE
  • 18
    Gunther S, Piwon N, Will H. Wild type levels of pregenomic RNA and replication but reduced pre-C RNA and e-antigen synthesis of hepatitis B virus with C(1653)-T, A(1762) -T, and G(1764)-A mutations in the core promoter. J Gen Virol 1998; 79:375–380.MEDLINE
  • 19
    Moriyama K, Okamoto H, Tsuda F, Mayumi M. Reduced precore transcription and enhanced core-pregenome transcription of hepatitis B virus DNA after replacement of the precore-core promoter with sequences associated with e antigen-seronegative persistent infections. Virology 1996; 226:269–280.MEDLINE
  • 20
    Nishizono A, Hiraga M, Kohno K, Sonoda YT, Terao H, Fujioka T, Nasu M, et al. Mutations in the core promoter/enhancer II regions of naturally occurring hepatitis B virus variants and analysis of the effects on transcription activities. Intervirology 1995; 38:290–294.MEDLINE
  • 21
    Liang TJ, Hasegawa K, Rimon N, Wands JR, Ben-Porath B. A hepatitis B virus mutant associated with an epidemic of fulminant hepatitis. N Engl J Med 1991; 324:1705–1709.MEDLINE
  • 22
    Omata M, Ehata T, Yokosuka O, Hosoda K, Ohto M. Mutations in the precore region of hepatitis B virus DNA in patients with fulminant and severe hepatitis. N Engl J Med 1991; 324:1699–1704.MEDLINE
  • 23
    Tur-Kaspa R, Klein A, Aharonson S. Hepatitis B virus precore mutants are identical in carriers from various ethnic origins and are associated with a range of liver disease severity. Hepatology 1992; 16:1338–1342.MEDLINE
    Direct Link:
  • 24
    Akarca US, Greene S, Lok ASF. Detection of precore hepatitis B virus mutants in asymptomatic HBsAg-positive family members. Hepatology 1994; 19:1366–1370.MEDLINE
    Direct Link:
  • 25
    Sato S, Suzuki K, Akahane Y, Akamatsu K, Akiyama K, Yunomura K, Tsuda F, et al. Hepatitis B virus strain with mutation in the core promoter in patients with fulminant hepatitis. Ann Intern Med 1995; 122:241–248.MEDLINE
  • 26
    Lok ASF, Lai CL, Wu PC, Leung EKY. Long-term follow-up in a randomized controlled trial of recombinant alpha-interferon in Chinese patients with chronic hepatitis B infection. Lancet 1988; 2:298–302.MEDLINE
  • 27
    Lok ASF, Wu PC, Lai CL, Lau JYN, Leung EKY, Wong LSK, Ma OCK, et al. A controlled trial of interferon with or without prednisone priming for chronic hepatitis B. Gastroenterology 1992; 102:2091–2097.MEDLINE
  • 28
    Buckwold VE, Xu Z, Yen B, Ou JH. Effects of a frequent double-nucleotide basal core promoter mutation and its putative single-nucleotide precursor mutations on hepatitis B virus gene expression and replication. J Gen Virol 1997; 78:2055–2065.MEDLINE
  • 29
    Laskus T, Rakela J, Nowicki MJ, Persing DH. Hepatitis B virus core promoter sequence analysis in fulminant and chronic hepatitis B. Gastroenterology 1995; 109:1618–1623.MEDLINE
  • 30
    Kidd AH, Kidd-Ljunggren K. A revised secondary structure model for the 3′-end of hepatitis B virus pregenomic RNA. Nucleic Acids Res 1996; 24:3295–3301.MEDLINE
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