Nitric oxide production and inducible nitric oxide synthase expression in peritoneal macrophages of cirrhotic patients

Authors

  • Wladimiro Jiménez,

    Corresponding author
    1. Hormonal Laboratory, Hospital Clínic Universitari and Institut d'Investigacions Biomèdiques August Pi i Sunyer (IDIBAPS) and Instituto Reina Sofia de Investigaciones Nefrológicas (IRSIN), University of Barcelona, Barcelona, Spain
    • Laboratorio Hormonal, Hospital Clinic Universitari, Villarroel 170, Barcelona 08036, Spain. fax: 3493-4515272
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  • Josefa Ros,

    1. Hormonal Laboratory, Hospital Clínic Universitari and Institut d'Investigacions Biomèdiques August Pi i Sunyer (IDIBAPS) and Instituto Reina Sofia de Investigaciones Nefrológicas (IRSIN), University of Barcelona, Barcelona, Spain
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  • Manuel Morales-Ruiz,

    1. Hormonal Laboratory, Hospital Clínic Universitari and Institut d'Investigacions Biomèdiques August Pi i Sunyer (IDIBAPS) and Instituto Reina Sofia de Investigaciones Nefrológicas (IRSIN), University of Barcelona, Barcelona, Spain
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  • Miguel Navasa,

    1. Liver Unit, Hospital Clínic Universitari and Institut d'Investigacions Biomèdiques August Pi i Sunyer (IDIBAPS) and Instituto Reina Sofia de Investigaciones Nefrológicas (IRSIN), University of Barcelona, Barcelona, Spain
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  • Manuel Solé,

    1. Pathology Department, Hospital Clínic Universitari and Institut d'Investigacions Biomèdiques August Pi i Sunyer (IDIBAPS) and Instituto Reina Sofia de Investigaciones Nefrológicas (IRSIN), University of Barcelona, Barcelona, Spain
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  • Jordi Colmenero,

    1. Liver Unit, Hospital Clínic Universitari and Institut d'Investigacions Biomèdiques August Pi i Sunyer (IDIBAPS) and Instituto Reina Sofia de Investigaciones Nefrológicas (IRSIN), University of Barcelona, Barcelona, Spain
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  • Pau Sort,

    1. Liver Unit, Hospital Clínic Universitari and Institut d'Investigacions Biomèdiques August Pi i Sunyer (IDIBAPS) and Instituto Reina Sofia de Investigaciones Nefrológicas (IRSIN), University of Barcelona, Barcelona, Spain
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  • Francisca Rivera,

    1. Hormonal Laboratory, Hospital Clínic Universitari and Institut d'Investigacions Biomèdiques August Pi i Sunyer (IDIBAPS) and Instituto Reina Sofia de Investigaciones Nefrológicas (IRSIN), University of Barcelona, Barcelona, Spain
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  • Vicente Arroyo,

    1. Liver Unit, Hospital Clínic Universitari and Institut d'Investigacions Biomèdiques August Pi i Sunyer (IDIBAPS) and Instituto Reina Sofia de Investigaciones Nefrológicas (IRSIN), University of Barcelona, Barcelona, Spain
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  • Juan Rodés

    1. Liver Unit, Hospital Clínic Universitari and Institut d'Investigacions Biomèdiques August Pi i Sunyer (IDIBAPS) and Instituto Reina Sofia de Investigaciones Nefrológicas (IRSIN), University of Barcelona, Barcelona, Spain
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Abstract

The present study assessed whether peritoneal macrophages isolated from cirrhotic patients produce nitric oxide (NO) and express NO synthase type II (NOS II) mRNA and protein. Patients with cirrhosis and ascites without peritonitis or with unresolved or resolved spontaneous bacterial peritonitis (SBP) were studied. Following paracentesis, ascites NOmath image + NOmath image content (NOx) was measured. Peritoneal macrophages from ascites were seeded on well plates, and NOmath image in the medium was determined. NOx was higher in patients with unresolved or resolved SBP than in cirrhotic patients without peritonitis. Macrophages of patients with SBP or resolved SBP produced NOmath image after 30 hours in culture, but those obtained from patients without peritonitis did not. Reverse-transcription polymerase chain reaction (RT-PCR) and immunocytochemical analysis revealed the presence of a clear signal for NOS II mRNA and protein in macrophages of SBP patients, regardless of whether or not the infection subsided. Therefore, peritoneal macrophages isolated from cirrhotic patients with unresolved or resolved SBP produce NO and express the NOS II mRNA and protein, suggesting that NOS II may contribute to the control of SBP, or to its associated pathology, in human cirrhosis.

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