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Additional Supporting Information may be found in the online version of this article.

FilenameFormatSizeDescription
HIPO_20838_sm_suppfig1.tif899KSupporting Figure 1. Calibration curves for the primer sets. A) Calibration curve for arc. Cycle threshold (Ct) values from qPCR analysis are plotted against different levels of dilution of RNA input (undiluted, 1/5, 1/25, and 1/125). To generate the standard cDNA, we reverse-transcribed 2 μg total RNA extracted from whole hippocampus under identical conditions and at the same time at which we reverse-transcribed total RNA extracted from experimental and control CA1 tissue samples, as described in the Methods. Based on the slope of the calibration curve, the amplification efficiency for the arc primer set was estimated to be 1.96. B) Calibration curve for zif368. Similar data as shown in A. Based on the slope of the calibration curve, the amplification efficiency for the zif268 primer set was estimated to be 1.98. C) Calibration curve for gapdh. Similar data as shown in A. Based on the slope of the calibration curve, the amplification efficiency for the gapdh primer set was estimated to be 1.97.
HIPO_20838_sm_supptab1.doc54KSupporting Table 1. Mean Ct values used for calculation of the relative arc mRNA levels.
HIPO_20838_sm_supptab2.doc53KSupporting Table 2. Mean Ct values used for calculation of the relative zif268 mRNA levels.

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