LTP- and LTD-inducing stimulations cause opposite changes in arc/arg3.1 mRNA level in hippocampal area CA1 in vivo
Version of Record online: 7 SEP 2010
Copyright © 2010 Wiley Periodicals, Inc.
Volume 21, Issue 12, pages 1290–1301, December 2011
How to Cite
Yilmaz-Rastoder, E., Miyamae, T., Braun, A. E. and Thiels, E. (2011), LTP- and LTD-inducing stimulations cause opposite changes in arc/arg3.1 mRNA level in hippocampal area CA1 in vivo. Hippocampus, 21: 1290–1301. doi: 10.1002/hipo.20838
- Issue online: 23 NOV 2011
- Version of Record online: 7 SEP 2010
- Manuscript Accepted: 19 MAY 2010
- National Institute of Neurological Disorders and Stroke (NINDS). Grant Number: R01–046423
Additional Supporting Information may be found in the online version of this article.
|HIPO_20838_sm_suppfig1.tif||899K||Supporting Figure 1. Calibration curves for the primer sets. A) Calibration curve for arc. Cycle threshold (Ct) values from qPCR analysis are plotted against different levels of dilution of RNA input (undiluted, 1/5, 1/25, and 1/125). To generate the standard cDNA, we reverse-transcribed 2 μg total RNA extracted from whole hippocampus under identical conditions and at the same time at which we reverse-transcribed total RNA extracted from experimental and control CA1 tissue samples, as described in the Methods. Based on the slope of the calibration curve, the amplification efficiency for the arc primer set was estimated to be 1.96. B) Calibration curve for zif368. Similar data as shown in A. Based on the slope of the calibration curve, the amplification efficiency for the zif268 primer set was estimated to be 1.98. C) Calibration curve for gapdh. Similar data as shown in A. Based on the slope of the calibration curve, the amplification efficiency for the gapdh primer set was estimated to be 1.97.|
|HIPO_20838_sm_supptab1.doc||54K||Supporting Table 1. Mean Ct values used for calculation of the relative arc mRNA levels.|
|HIPO_20838_sm_supptab2.doc||53K||Supporting Table 2. Mean Ct values used for calculation of the relative zif268 mRNA levels.|
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