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Additional Supporting Information may be found in the online version of this article.

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hipo22128-sup-0001-suppfig1.tif2157KSupplementary Figure 1, (A) PDE10A levels were analyzed by Western blot of nuclear (Nuc) and cytosolic (Cyt) enriched fractions obtained from 12-week-old WT mice hippocampus using an antibody against the C-terminal region of the protein pre-incubated with the blocking peptide (Santa Cruz Biotechnology; 1:200: 2 h at room temperature), which resulted in loss of signal. (B) PDE10A levels were analyzed by Western blot of nuclear (Nuc) and cytosolic (Cyt) enriched fractions obtained from 12-week-old WT and R6/1 mice hippocampus using an antibody against the N-terminal region of the protein. Representative immunoblots are shown.
hipo22128-sup-0002-suppfig2.tif60KSupplementary Figure 2. Chronic papaverine treatment does not alter hippocampal pERIC1/2, BDNF, TrkB or c-fos protein levels. pERK1/2 (A), BDNF (B), TrkB (C) and c-fos (D) levels were analyzed by Western blot of hippocampal samples obtained from WT and R6/1 mice chronically treated with papaverine (20 mg/kg) or vehicle (23 days; i.p.). Values (obtained by densitometric analysis of Western blot data) are expressed as percentage of WT vehicle-treated mice (after normalization with α-tubulin, except for pERK1/2: phosphorylated protein levels/total protein levels after normalization with α-tubulin), and shown as mean ± SEM (n=5–7). Data were analyzed by two-way ANOVA.
hipo22128-sup-0003-suppfig3.tif2018KSupplementary Figure 3. CRTC1 levels are reduced in the hippocampus of R6/1 mice. Total (A) and nuclear (B) CRTCI levels, as well as exportin-1 levels (C) were analyzed by Western blot of hippocampal samples obtained from 12-week-old WT and R6/1 mice. Representative immunoblots are shown. Values (obtained by densitometric analysis of Western blot data) are expressed as percentage of WI mice (CRTC1/α-tubulin. CRTC1/lamin B and exportin-1/α-tubulin ratio, respectively), and shown as mean ± SEM. Data were analyzed by Student's t-test. *p<0.05 and **p<0.01 as compared with WT mice. Cyt: Cytosol-enriched fractions; Nuc: Nuclear-enriched fractions.

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