Detection of point mutations by solid-phase methods

Authors

  • Ann-Christine Syvänen,

    Corresponding author
    1. Department of Human Molecular Genetics, National Public Health Institute, Mannerheimintie 166, SF-00300 Helsinki, Finland; Fax: 358-0474-4480
    • Department of Human Molecular Genetics, National Public Health Institute, Mannerheimintie 166, SF-00300 Helsinki, Finland; Fax: 358-0474-4480
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  • Ulf Landegren

    1. Department of Medical Genetics, Box 589, Uppsala Biomedical Center, S-75123 Uppsala, Sweden; Fax: 358-0474-4480
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Abstract

Several techniques exist that permit the efficient distinction among characterized DNA sequence variants. In this review we discuss a number of such analytic procedures. These techniques all take advantage of a variety solid supports to prepare and analyze reaction products. The described diagnostic principles are now being applied for the development of miniaturized assay formats, suitable for automated detection of large sets of sequences in clinical samples. © 1994 Wiley-Liss, Inc.

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